富硫废水中稳定生物单质硫效果与机制研究

doi:10.19965/j.cnki.iwt.2023-0930

工业水处理 ›› 2024, Vol. 44 ›› Issue (9): 67-74. doi: 10.19965/j.cnki.iwt.2023-0930

富硫废水中稳定生物单质硫效果与机制研究

徐金兰¹^,²^,³(), 薛淑君¹^,²^,³, 曹泽壮¹^,²^,³, 巩丽霞¹^,²^,³, 曹芬¹^,²^,³

^1. 西安建筑科技大学环境与市政工程学院，陕西西安 710055
^2. 陕西省环境工程重点实验室，陕西西安 710055
^3. 西北水资源与环境生态教育部重点实验室，陕西西安 710055

收稿日期:2024-07-03 出版日期:2024-09-20 发布日期:2024-09-26
作者简介:
徐金兰（1973— ），教授，博士生导师。E-mail：xujinlan@ xauat.edu.cn。
基金资助:
国家自然科学基金项目(51778524)

Mechanism and effect insight on stabilizing biological S⁰ in sulfur-rich wastewater

Jinlan XU¹^,²^,³(), Shujun XUE¹^,²^,³, Zezhuang CAO¹^,²^,³, Lixia GONG¹^,²^,³, Fen CAO¹^,²^,³

^1. School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China
^2. Shaanxi Key Laboratory of Environmental Engineering, Xi’an 710055, China
^3. Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, Xi’an 710055, China

Received:2024-07-03 Online:2024-09-20 Published:2024-09-26

摘要/Abstract

摘要：

为提高含硫废水同步脱氮除硫工艺中生物单质硫回收率，开展了富硫废水中稳定生物单质硫效果与机制研究。在富硫废水（S^2-质量浓度300 mg/L）和贫硫废水（S^2-质量浓度200、100 mg/L）中添加阻断剂硫代硫酸钠进行脱氮除硫实验。结果表明，富硫废水中稳定组（加30 mg/L阻断剂）在60 h时单质硫积累量达到68.79 mg/L，比未稳定组（不添加阻断剂）高25.34 mg/L，单质硫稳定率达58.31%，单质硫回收率为36.84%；而贫硫废水（S^2-质量浓度为200、100 mg/L）中单质硫不稳定，单质硫稳定率仅11.2%、14.14%，且回收率低（15.71%、12.39%）。可见，阻断剂在富硫废水中实现了稳定生物单质硫。此外，阻断剂有利于反硝化菌和脱氮硫杆菌增殖积累，富硫废水下高比例的脱氮硫杆菌可促进单质硫稳定。阻断剂抑制了硫代硫酸盐和硫酸盐的生成，使硝酸盐得到更多电子（稳定组比未稳定组多得19.76 mmol/L电子），促进了单质硫的生成，实现了生物单质硫稳定。该技术可为废水中生物单质硫的回收与利用提供理论基础。

关键词: 生物单质硫, 阻断剂, 脱氮硫杆菌, 富硫废水

Abstract:

To improve the recovery rate of biological elemental sulfur(S⁰) in the simultaneous denitrification and sulfur removal process in sulfur wastewater, the effect and mechanism of stabilizing biological S⁰ in sulfur-rich wastewater were studied. The experiments were conducted using blockers to stable biological S⁰ in sulfur-rich wastewater(300 mg/L S^2-) and sulfur-poor wastewater(200, 100 mg/L S^2- ). It was found that in sulfur-rich wastewater, 68.79 mg/L of S⁰ was accumulated in the stable group (adding 30 mg/L blocker) at 60 h, which was 25.34 mg/L higher than that in the unstable group (adding 0 mg/L blocker), the S⁰ stability rate reached 58.31% and the S⁰recovery rate was 36.84%. However, the S⁰ in sulfur-poor wastewater (200, 100 mg/L S^2-) was unstable, the S⁰ stability rate was only 11.2%, 14.14%, and the S⁰recovery rate was low (15.71%, 12.39%). It could be seen that the blockers stabilized the biological S⁰ in sulfur-rich wastewater. Additionally, the blockers were conducive to the accumulation of denitrifying bacteria and Thiobacillus denitrificans, and the high proportion of Thiobacillus denitrificans was conducive to stable S⁰in sulfur-rich wastewater. The blockers inhibited the formation of thiosulfate and sulfate and made nitrate get more electrons(19.76 mmol/L more electrons in stable group than in unstable group), promoting the generation and stability of biological S⁰. This technology could provide a theoretical basis for the recovery and utilization of biological S⁰ in wastewater.

Key words: biological elemental sulfur, blockers, Thiobacillus denitrificans, sulfur-rich wastewater

中图分类号:

X703

徐金兰, 薛淑君, 曹泽壮, 巩丽霞, 曹芬. 富硫废水中稳定生物单质硫效果与机制研究[J]. 工业水处理, 2024, 44(9): 67-74.

Jinlan XU, Shujun XUE, Zezhuang CAO, Lixia GONG, Fen CAO. Mechanism and effect insight on stabilizing biological S⁰ in sulfur-rich wastewater[J]. Industrial Water Treatment, 2024, 44(9): 67-74.

https://www.iwt.cn/CN/Y2024/V44/I9/67

图/表 7

图1 反应器装置

Fig.1 Reactor installation

图2 S⁰产量

Fig.2 S⁰production

表1 单质硫积累量和硫酸盐质量浓度

Table 1 Total sulfur accumulation and sulfate concentration

废水类型	单质硫积累量/（mg·L^-1）		硫酸盐质量浓度/（mg·L^-1）
废水类型	稳定组	未稳定组	稳定组	未稳定组
富硫废水	66.72	63.10	114.29	139.05
S^2-为200 mg/L的贫硫废水	69.21	59.30	83.41	67.44
S^2-为100 mg/L的贫硫废水	23.37	29.54	48.22	40.45

图3 细菌数量、反硝化细菌与脱氮硫杆菌数量比及其差值、稳定系数对比

Fig.3 Bacteria quantity，the ratio of denitrifying bacteria to Thiobacillus denitrificans and their difference，stability coefficient

图4 微生物群落FISH扫描图片

Fig.4 FISH scan images of microbial communities

图5 硝酸盐消耗分配

Fig.5 Distribution of nitrate consumption

图6 电子衡算

Fig.6 Electronic calculations

参考文献 18

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