硫自养反硝化ASBR工艺脱氮除硫效果及功能菌研究

doi:10.19965/j.cnki.iwt.2023-0794

摘要/Abstract

摘要：

研究了硫自养反硝化-厌氧序批式反应器（SDAD-ASBR）同步脱氮除硫效果的影响因素和SDAD-ASBR中微生物群落结构特征。结果表明，当n（S）/n（N）（记作S/N）为1，进水S^2-和NO₃ ^--N质量浓度分别为300 mg/L和131.25 mg/L时系统内无亚硝酸盐积累现象，脱氮除硫效率高，硫化物和硝酸盐的去除率分别为99.97%和84.34%；但S/N降低，出水产物中亚硝酸盐升高。水力停留时间（HRT）对硝酸盐的去除效果影响显著，但对硫化物的去除效果影响不大，当HRT由12 h缩短至2.4 h时，硫化物的去除率均在99%以上，而硝酸盐的去除率则由93.14%下降至77.04%。系统中脱氮除硫的主要功能菌属为微小杆菌属、梭菌属、芽孢杆菌属、硫杆菌属和鞘单胞菌属。

关键词: 硫化物自养反硝化, 厌氧序批式反应器, 脱氮除硫, 功能菌

Abstract:

In this paper, the influence factors of nitrogen and sulphur removal effect and main functional bacteria were investigated in a sulfide autotrophic denitrification-anaerobic sequencing batch reactor(SDAD-ASBR). The results showed that when n(S)/n(N)(recorded as S/N) was 1, and the influent S^2- and NO₃ ^--N were 300 mg/L and 131.25 mg/L, respectively, there was no nitrite formation, and the sulfides and nitrates removal rates were 99.97% and 84.34%, respectively. However, as the S/N was reduced, the concentration of nitrite in the effluent increased. The hydraulic retention time(HRT) had a significant effect on the removal of nitrate, but had little effect on the sulphide removal. When HRT was shortened from 12 h to 2.4 h, the removal rates of sulfide were all above 99%, while the removal rate of nitrate decreased from 93.14% to 77.04%. The main functional genera for nitrogen and sulphur removal in the system were Exiguobacterium, Clostridium, Bacillus, Thiobacillus and Sphingomonas.

Key words: sulfur-driven autotrophic denitrification, anaerobic sequencing batch reactor, nitrate and sulfide removal, functional bacteria

中图分类号:

X703.1

严华, 郭昌梓, 赵婉琴, 张旭立, 韩义, 王一旭. 硫自养反硝化ASBR工艺脱氮除硫效果及功能菌研究[J]. 工业水处理, 2024, 44(10): 151-157.

Hua YAN, Changzi GUO, Wanqin ZHAO, Xuli ZHANG, Yi HAN, Yixu WANG. Simultaneous nitrogen and sulphur removal effect and functional bacteria of sulfur-driven autotrophic denitrification ASBR process[J]. Industrial Water Treatment, 2024, 44(10): 151-157.

https://www.iwt.cn/CN/Y2024/V44/I10/151

图/表 5

图1 S/N对SDAD-ASBR工艺脱氮除硫效果的影响

Fig.1 Effect of S/N on the sulfide and nitrogen removal by SDAD-ASBR process

图2 S/N为1时进水S^2-和NO₃ ^--N质量浓度对脱氮除硫效果的影响

Fig.2 The effect of S^2- and NO₃ ^--N mass concentrations in influent on sulfide and nitrogen removal efficiencies with S/N of 1

图3 HRT对SDAD-ASBR工艺脱氮除硫效果的影响

Fig.3 Effect of HRT on the sulfide and nitrogen removal by SDAD-ASBR process

图4 HRT为8 h时反应器1个周期的脱氮除硫效果

Fig.4 Sulfide and nitrogen removal efficiencies of the reactor for one cycle at HRT of 8 h

图5 功能菌群在门、属水平的丰度分布

Fig.5 The abundance distribution of functional microbial communities at the phylum and genus levels

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