厌氧氨氧化菌富集培养技术关键与影响因素

doi:10.11894/1005-829x.2013.33(10).6

摘要/Abstract

摘要：

与传统的硝化-反硝化脱氮工艺相比,厌氧氨氧化工艺在高氨氮污水脱氮方面具有明显的优势。但由于厌氧氨氧化菌倍增时间长,对环境条件敏感,导致厌氧氨氧化工艺的启动时间较长,限制了该工艺的推广应用。回顾了近年来厌氧氨氧化菌富集培养技术的研究进展,分析了进水基质、接种污泥类型与反应器类型对厌氧氨氧化启动过程的影响,对富集厌氧氨氧化菌的技术关键进行了总结。该项研究对厌氧氨氧化工艺的工程应用具有技术指导作用。

关键词: 厌氧氨氧化, 富集培养, 基质抑制, 接种污泥, 反应器类型

Abstract:

The anaerobic ammonia oxidation(ANAMMOX) process is a novel and promising alternative to conventional nitrogen removing processes，especially for treating ammonium/nitrogen-rich wastewaters. However,the requirement for a relatively longer start-up time restricts the application of ANAMMOX processes due to a very long doubling time of ANAMMOX bacteria and their susceptibility to environmental conditions. After reviewing the research progress in the enrichment techniques of ANAMMOX bacteria in recent years，and the effects of influent substrates，inoculation sludge types and reactor configurations on the start-up courses of anaerobic ammonia oxidation are analyzed. The technical essentials of the enrichment of ANAMMOX bacteria are summarized. The research could serve as a technical instruction on the application of ANAMMOX process.

Key words: anaerobic ammonia oxidization (ANAMMOX), enrichment, substrate inhibition, inoculation sludge, reactor configuration

中图分类号:

X703

沈冰洁, 王荣昌. 厌氧氨氧化菌富集培养技术关键与影响因素[J]. 工业水处理, 2013, 33(10): 6-10.

Shen Bingjie, Wang Rongchang. Technical essentials and influencing factors of the enrichment of anaerobic ammonia oxidation(ANAMMOX)bacteria[J]. INDUSTRIAL WATER TREATMENT, 2013, 33(10): 6-10.

https://www.iwt.cn/CN/Y2013/V33/I10/6

参考文献

[1] Strous M, Heijnen J J, Kuenen J G, et al. The、‘quencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammoniumoxidizing microorganisms[J]. Appl. Microbial. Biotechnol.,1998,50(5):589-596.
[2] Dapena-Mora A,Fernandez I,Campos J L,et al. Evaluation oFactivity and inhibition effects on Anammox process by batch tests based on the nitrogen gas production[J]. Enzyme and Microbial Technoloiry,2007,40(4):859-865.
[3] Fernandez I,Dosta J,Fajardo C,et al. Short- and long-term effects of ammonium and nitrite on the Anammox process[J].J. Environ.Managc.,2010,95:S170-S174.
[4] Waki M, Tokutomi T，Yokoyama H，et al. Nitrogen removal from animal waste treatment water by anammox enrichment[J]. Bioresource Technology,2007,98(14):2775-2780.
[5] Jung J Y,Kang 5 H,Chung Y C，et al. Factors affecting the activity of anammox bacteria during start up in the continuous culture reactor[J].Watcr Sci. Technol. ,2007, 55 (1/2 ) :459-468.
[6] Tang C J，Zheng P，Mahmood Q，et al. Start一up and inhibition analysis of the Anammox process seeded with anaerobic granular sludge[J]. J. Ind. Microbial. Biotechno1.,2009,36(8):1093-1100.
[7] 阳广风，沈李东，金仁村，等.新型厌氧氨氧化反应器脱氮性能及过程动力学特性的研究[J].环境科学学报，2011,31(10);2138-2145.
[8] Tsushimx I,Ogxsxwxrx Y,KindxichiT,ct al. Development of high-rate anaerobic ammonium-oxidizing (xnxmmox) biofilm rexctors[J].Wxtcr Rcsexrch,2007,41(8):1623-1634.
[9] Strous M , Kucncn G, Jetten M. Key physiology of anaerobic ammonium oxidation [J]. Applied and Environmental Microbiology,1999,65(7):3248-3250.
[10] Kimurx Y,Isxkx K,Kxzxmx F,ct al. Effects of nitrite inhibition on anaerobic ammonium oxidation [J]. Appl. Microbial. Biotcchnol.，2010,86(1);359-365.
[11] Cho S,Txkxhxshi Y,Fujii N,ct al. Nitrogen removal pcrlormxnc‘ and microbial community analysis of an anaerobic up-flow granular bed anammox reactor [J]. Chcmosphcrc , 2010 , 78 (9 ) : 1129-1135.
[12] Loth T,vxn dcr Stir W R L,Kleerebezem R,et al. The‘伍(t of nitrite inhibition on the anammox process[J].Wxtcr Research,2012,46(8):2559-2569.
[13] Anthonisen A C , Lochr R C , Prxkxsxm T B S，‘t al. Inhibition of nitrification by ammonia and nitrous acid [J].Wxtcr Pollution Control Federation ,1976 , 48 ( 5):835-852.
[14] Schmidt I，Back E. Anaerobic ammonia oxidation with nitrogen dioxide by Nitro.soraonas eutrophajJj. Arch.Microbial. 1997,167(2/3):106一111.
[15] 马富国，张树军，曹相生，等.硝化生物膜启动厌氧氨氧化反应器的研究fJ7.中国给水排水，2008,24(23):24-28.
[16] 赵宗升，赵云霞，陈智均，等.厌氧氨氧化菌接种污泥的选择培养过程研究[J].环境工程学报，2007,1(2):39-42.
[17] 金仁村，郑平，陈旭良，等.厌氧氨氧化反应器快速启动力法的探讨[J7.化工进展,2005,24(6):629-631.
[18] 张少辉，郑平，华玉妹，等.反硝化生物膜启动厌氧氨氧化反应器的研究[J].环境科学学报，2004,24(2);220-224.
[19] 唐崇俭，郑平，陈建伟，等.中试厌氧氨氧化反应器的启动与调控[J].生物工程学报，2009,25(3):406-412.
[20] 」丁爽，郑平，唐崇俭，等.小同接种物启动Anammox -EGSB反应器的污泥性能[J].化工学报，2011,62(5):1395-1401.
[21] 李祥，黄勇，袁怡，等.小同泥源对厌氧氨氧化反应器启动的影响[J].环境工程学报，2012,6(7):2143-2148.
[22] 冯平，周少奇.常温h厌氧氨氧化生物膜反应器的启动研究[J].环境科学与技术，2010,33(6):19-22.
[23] Shcn Lidong,Hu Anhui,Jin Rcncun,ct al. Enrichment of anammox bacteria from three sludt;c sources for the startup of monosodiumglutamate industrial wastewater treatment system [J]. J.Hazard. Mater. , 2012 ,199/200 .193-199.
[24] Chamchoi N，Nitisoravut S. Anammox enrichment from different conventional sludges [J]. Chemosphere ,2007 , 66 (11):2225-2232.
[25] Kucnen J(;.Anammox bacteria;From discovery to application [J].Nat. Rev. Microbio1.,2008,6(4):320-326.
[26] Egli K,Fanger U,Alvarez P J J,et al. Enrichment and characterizalion of an anammox bacterium from a rotating biological(ontactor treating ammonium-rich leachate[J]. Arch. Microbio1.,2001，175(3):198-207.
[27] Mulder A,Vandegraaf A A, Robertson L A,et al. Anaerobic ammonium oxidation discovered in a denitrifyingfluidized-bed reactor[J].FEMS Microbiology Ecology,1995，16(3):177-183.
[28] Ahn Y H , Hwang I S，Min K S. ANAMMOX and partial denitritalion in anaerobic nitrogen removal from piggery waste[J].Watcr Sci. Techno1.,2004,49(5/6):145-153.
[29] Trigo C,Campos J L,Garrido J M,et al. Start-up of the Anamm process in a membrane bioreactor [J]. Journal of Biotechnoloiry,2006,126(4):475-487.
[30] 路青，李秀芬，华兆ar等.接种污泥源对厌氧氨氧化启动效能的影响[J].生态与农村环境学报2009,25(4);60-65.
[31] Strous M , Kuenen J G , Fuerst J A , et al. The anammox case ; A new xperimental manifesto for microbiological‘(ophysioloiry [J]. Antonic van Lccuwenhock , 2002 , 81(1/2/3/4 ) : 693-702.
[32] Wyffels S,Boeckx P,Pynaert K,et al. Nitrogen removal from sludge reject water by a two-stage oxygen-limited autotrophic nitrification denitrification process[J]. Water Sci. Techno1.,2004,49(5/6):57-64.
[33] Tao Yu,Gao Dawen,Fu Yuan,et al. Impact of reactor configuration on anammox process start-up;MBR versus SBR [J]. Bioresourcc Technoloiry , 2012 ,104 ; 73-80.
[34] Fernandez I,Vazquez-Padin J R,Mosquera-Corral A,et al. Biofilm xnd granular systems to improve Anammox hiomass retention [J].Biochem. Eng. J.,2008,42(3):308-313.
[35] 唐崇俭，郑平，张蕾.厌氧氨氧化菌富集培养技术的研究与应用[J]一化工进展,2009,28(8):1421-1426.

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