Determination of dehydrogenase activity and its application

doi:10.11894/1005-829x.2008.28.10

INDUSTRIAL WATER TREATMENT ›› 2008, Vol. 28 ›› Issue (10): 1-4. doi: 10.11894/1005-829x.2008.28.10

Determination of dehydrogenase activity and its application

Dun Mina¹, Hu Wenrong^1,2, Pei Haiyan³, Xie Jun^1,4

1. School of Environmental Science and Engineering, Shandong University, Jinan 250100, China;
2. Research Centre of Environmental Science and Engineering, Jinan 250100, China;
3. School of Environmental Science and Engineering, Ocean University of China, Qingdao 266003, China;
4. Shandong Province Environmental Monitoring Centre, Jinan 250013, China

Received:2008-03-05 Revised:2008-03-05 Online:2008-10-20 Published:2010-10-01

脱氢酶活性检测方法及应用

顿咪娜¹, 胡文容^1,2, 裴海燕³, 解军^1,4

1. 山东大学环境科学与工程学院, 山东济南２５０１００;
2. 山东省环境科学工程技术研究中心, 山东济南２５０１００;
3. 中国海洋大学环境科学与工程学院, 山东青岛 266003;
4. 山东省环境监测中心站, 山东济南 250013

作者简介:顿咪娜（1983- ）, 2006 级山东大学在读硕士研究生。电话： 15953178294, E- mail : dxiaomi@yahoo.cn.
基金资助:
山东省自然科学基金项目(Z2006B04)；山东省中青年科学家奖励基金项目(2006BS08001)；山东省环保局项目(hcyf0602)

Abstract

Abstract:

The determination of dehydrogenase activity （ DHA ） by using 2,3,5-tripheyltetrazolium （ TTC ） and p-iodonitrotetrazolium violet （ INT ） as artificial hydrogen-accepted body, and their advantages and disadvantages inreflecting microbial activity are introduced. The development of TTC-dehydrogenase activity determination is re-viewed. Its standard curve in the determination process of dehydrogenase activity by using TTC as hydrogen ac-cepted body is discussed particularly. A great many of influential factors, such as temperature, pH and extractantetc. are compared and analyzed. The best experimental conditions are confirmed. The application of this process to wastewater treatment, the chemical poisons and metallic poisons and soil pollution are summarized briefly.

Key words: dehydrogenase activity, 2, 3, 5-tripheyltetrazolium, p-iodonitrotetrazolium violet

摘要：

介绍了分别用２, ３, ５－氯化三苯基四氮唑（ＴＴＣ）以及碘硝基四唑紫（ＩＮＴ）等作为人工受氢体进行脱氢酶活性（ＤＨＡ）检测的几种常见方法，以及它们自身在反映微生物活性等方面的优缺点。论述了ＴＴＣ在ＤＨＡ检测中的发展，重点讨论了ＴＴＣ作为受氢体进行ＤＨＡ测定过程中其标准曲线制作方法的改进，并对测定中诸多影响因素如温度、ｐＨ及萃取剂等进行了比较分析，确定了最佳实验条件。简要概括了目前ＤＨＡ测定方法在废水生化处理、评价化学毒物和金属毒物的毒性、土壤污染等方面的应用。

关键词: 脱氢酶活性, 2, ３, 5- 氯化三苯基四氮唑, 碘硝基四唑紫

CLC Number:

Dun Mina, Hu Wenrong, Pei Haiyan, Xie Jun. Determination of dehydrogenase activity and its application[J]. INDUSTRIAL WATER TREATMENT, 2008, 28(10): 1-4.

顿咪娜, 胡文容, 裴海燕, 解军. 脱氢酶活性检测方法及应用[J]. 工业水处理, 2008, 28(10): 1-4.

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