Isolation and identification of an acid-resistant manganese-oxidizing bacteria and its Mn（Ⅱ） removal characteristics

doi:10.19965/j.cnki.iwt.2021-1146

Abstract

Abstract:

Mn（Ⅱ） removal from acid wastewater by microorganism has a wide application future. However，the activity of most Mn-oxidizing bacteria was poor under acidic conditions. In this study，a strain of acid-tolerant Mn-oxidizing bacteria was screened by plate scribing method from activated sludge in Xiangtan Wastewater Treatment Plant. The strain was identified by comparative analysis of 16S rDNA sequences. While，the effect of initial pollutant concentration，solution pH，and inoculum amount on Mn（Ⅱ） removal ability of this strain were investigated. Mn（Ⅱ） removal characteristics of the strain were comprehensively studied through the physicochemical characterization of product and kinetic processes of reaction. The results showed that the strain belonged to Klebsiella sp.，and it grew well at a concentration of 1 000 mg/L Mn（Ⅱ）. Mn（Ⅱ） removal rate could reach 98.93% at neutral pH. More importantly，52.20% of Mn（Ⅱ） could be removed by this strain at initial pH of 4，showing a strong acid resistance. Mn（Ⅱ） removal rate decreased with the increase of initial pollutant concentration，while increased with the increase of inoculum dose. SEM，XPS and FTIR characterization of the solid products showed that Mn-containing precipitates were produced on the surface of the bacteria，and Mn（Ⅳ） and Mn（Ⅲ） were the main valence states in product. Kinetic analysis showed that the biological Mn removal process basically followed pseudo-first-order reaction，and the activity of the Mn-oxidizing bacteria was inhibited by high concentrations of Mn（Ⅱ）.

Key words: manganese oxidizing bacteria, acid tolerance, Mn（Ⅱ）, reaction process, mechanistic analysis

摘要：

生物氧化除锰法处理锰矿废水具有较好的应用前景，然而常见的锰氧化菌在酸性条件下活性差，以湘潭污水处理厂活性污泥为来源，通过平板划线法筛选耐酸性锰氧化菌，通过16SrDNA序列对比分析进行菌种鉴定，考察污染物初始浓度、溶液pH、接种量等对其去除Mn（Ⅱ）能力的影响，结合产物理化表征与动力学过程，全面研究该菌株的除锰行为。结果表明：该锰氧化菌菌株属于Klebsiella sp.，在Mn（Ⅱ）质量浓度为1 000 mg/L时依然长势良好；当pH为中性，氧化除锰率能达到98.93%，更重要的是pH为4时，除锰率仍能达到52.20%，表现出极强的耐酸性；随着Mn（Ⅱ）初始浓度的升高，菌株除锰率逐渐降低，然而随着接种量的增加，除锰率则升高。固相产物SEM、XPS与FTIR表征结果显示，菌体表面产生了含锰沉淀，且主要以Mn（Ⅳ）和Mn（Ⅲ）存在。动力学分析表明，生物除锰过程基本遵循假一级反应，高浓度Mn（Ⅱ）可以抑制锰氧化菌活性。

关键词: 锰氧化菌, 耐酸性, Mn（Ⅱ）, 反应过程, 机理分析

CLC Number:

703.1

Zhonggeng MO, Yongchao LI, Liping CHEN, Zheng XU. Isolation and identification of an acid-resistant manganese-oxidizing bacteria and its Mn（Ⅱ） removal characteristics[J]. Industrial Water Treatment, 2022, 42(9): 87-93.

莫忠耿, 李勇超, 陈丽苹, 徐政. 一株耐酸锰氧化菌的分离鉴定及去除Mn（Ⅱ）行为研究[J]. 工业水处理, 2022, 42(9): 87-93.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2021-1146

https://www.iwt.cn/EN/Y2022/V42/I9/87

Figures/Tables 10

Fig. 1 Colony characteristics and cell morphology of manganese oxidizing bacteria

Fig. 2 Phylogenetic tree based on 16SrDNA gene sequence

Fig. 3 Effect of pH on Mn（Ⅱ） removal rate

Fig. 4 Effect of Mn（Ⅱ） concentration on Mn（Ⅱ） removal of strain

Fig. 5 Effect of inoculation amount on Mn（Ⅱ） removal of strain

Fig. 6 Scanning electron micrographs and EDS results of manganese oxidation products from manganese oxidizing bacteria

Fig. 7 XPS spectrum of manganese removal products by manganese oxidizing bacteria（a） and high resolution spectrum of Mn 2p after reaction（b）

Fig. 8 FTIR results of manganese oxide products after manganese removal

Fig. 9 Residual manganese concentration changes with contact time

Table 1 Biomanganese oxide reaction kinetic parameters

C ₀/（mg·L^-1）	9.8	17.84	28.54	38.7	47.85
K/h^-1	0.027	0.013	0.010	0.007	0.004
R ²	0.993	0.904	0.948	0.963	0.928
$τ / h$	26.08	53.55	71.15	99.14	170.69

Table 1 Biomanganese oxide reaction kinetic parameters

C ₀/（mg·L^-1）	9.8	17.84	28.54	38.7	47.85
K/h^-1	0.027	0.013	0.010	0.007	0.004
R ²	0.993	0.904	0.948	0.963	0.928
$τ / h$	26.08	53.55	71.15	99.14	170.69

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