Study on the driving mechanism of efficient degradation of phenolic compounds by microorganisms mediated by phenol

doi:10.19965/j.cnki.iwt.2024-0229

Abstract

Abstract:

In this study, a strain that can degrade phenolic compounds was screened from aerobic activated sludge and named as Pseudomonas sp.LST11. The efficient degradation characteristics and driving mechanisms of this strain on two refractory phenolic compounds(2,6-dichlorophenol and p-chloro-m-cresol) under phenol-mediated degradation were experimentally investigated. The results showed that the inhibition period of 2,6-dichlorophenol and p-chloro-m-cresol on bacteria was shortened by 84 h and 96 h, and the average degradation rate was enhanced by about 5.9 and 7.6 times respectively, compared with that of the non-mediated group. The study further explored the mechanism and showed that the secretion of extracellular polymer substances and the level of total antioxidant capacity of the strain could be effectively elevated under the stimulation of phenol during the mediation period. The total amount of polysaccharides and proteins secreted by the strain reached to 87.7 mg/L and the total antioxidant capacity level reached to 2.11 mg/L. The total amount of polysaccharides and proteins secreted by the strains decreased to 17 mg/L and the level of total antioxidant capacity decreased to 1.26 mg/L after the mediated strain was transferred to 2,6-dichlorophenol for 4 h. The extracellular polymer substances secretion and the level of total antioxidant capacity of the strains were significantly reduced,and the strains showed a similar response profile to p-chloro-m-cresol, which showed that the strain could adapt to the toxic environment more quickly after being mediated by phenol. In addition, phenol mediation effectively contributed to the rise of phenol hydroxylase and catechol 2,3-dioxygenase activities to peaks 72-96 h earlier, and the activities were enhanced 1.58-1.88 times. The average degradation rates of 2,6-dichlorophenol and p-chloro-m-cresol were increased from 0.7 mg/(L·h) and 0.5 mg/(L·h) in 132 h to 4.1 mg/(L·h) and 3.8 mg/(L·h) in 24 h, respectively.

Key words: refractory phenolic compounds, mediate, efficient degradation, driving mechanism

摘要：

从好氧活性污泥中筛选出了一株能降解酚类化合物的菌株Pseudomonas sp. LST11，探究了在苯酚介导下该菌株对两种难降解性酚类化合物（2，6-二氯苯酚和对氯间甲酚）的高效降解特性及驱动机制。结果表明，与非介导组相比，2，6-二氯苯酚和对氯间甲酚对细菌的抑制期分别缩短84 h和96 h，平均降解速率分别提升约5.9倍和7.6倍。进一步探究机制表明，介导期内在苯酚的刺激下可以有效提升菌株胞外聚合物的分泌量和总抗氧化能力水平，菌株分泌的多糖和蛋白总量达到87.7 mg/L，总抗氧化能力水平达到2.11 mg/L。介导后的菌株转接至2，6-二氯苯酚4 h后，菌株分泌的多糖和蛋白总量降至17 mg/L，总抗氧化能力水平降至1.26 mg/L，菌株胞外聚合物分泌量和总抗氧化能力水平明显降低，对氯间甲酚表现出相似的响应特征，这是菌株经过介导后可以更快适应有毒环境的表现。另外苯酚介导使苯酚羟化酶和邻苯二酚双加氧酶活性上升至峰值的时间点提前72~96 h，活性增强1.58~1.88倍。2，6-二氯苯酚和对氯间甲酚的平均降解速率分别由132 h内的0.7 mg/（L·h）和0.5 mg/（L·h）提升至24 h内的4.1 mg/（L·h）和3.8 mg/（L·h）。

关键词: 难降解酚类化合物, 介导, 高效降解, 驱动机制

CLC Number:

X703.1

Huiqiang JIANG, Aining ZHANG, Yongjun LIU, Xingshe LIU, Lu YANG. Study on the driving mechanism of efficient degradation of phenolic compounds by microorganisms mediated by phenol[J]. Industrial Water Treatment, 2025, 45(4): 62-71.

蒋慧强, 张爱宁, 刘永军, 刘兴社, 杨璐. 苯酚介导下微生物高效降解酚类化合物驱动机制研究[J]. 工业水处理, 2025, 45(4): 62-71.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2024-0229

https://www.iwt.cn/EN/Y2025/V45/I4/62

Figures/Tables 8

Fig.1 Phylogenetic tree and SEM image of Pseudomonas sp.LST11

Fig.2 Metabolic properties of Pseudomonas sp. LST11 in response to different concentrations of target pollutants

Fig.3 Effect of mediation time and mediation concentration on the average degradation rate of refractory phenolic compounds

Fig.4 Degradation performance of Pseudomonas sp. LST11 by phenol-mediated on refractory phenolic compounds

Fig.5 Microbial secreted polysaccharide and protein content under optimal mediated conditions

Fig.6 Analysis of T-AOC secretion under optimal mediation conditions

Fig.7 Analysis of key enzyme activities under optimal mediation conditions

Fig.8 Driving mechanism of efficient degradation of refractory phenolic compounds by Pseudomonas sp. LST11 mediated by phenol

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