Cytotoxicity reduction characteristics during wastewater treatment process in typical industrial park

doi:10.19965/j.cnki.iwt.2021-1280

Abstract

Abstract:

Industrial wastewater has complex components and serious pollution，it is of great significance to study the toxicity reduction ability of industrial wastewater treatment process for water environment quality assurance. The cytotoxicity assays and fractionation analysis method were combined to analyze the toxic effects of water samples from two typical industrial park wastewater treatment plants. The results showed that the influent of both plants induced toxic effects on cell viability，induced intracellular reactive oxygen species and changed mitochondrial membrane potential，but the effluent did not have these above adverse effects. Meanwhile，the toxicity of wastewater in some treatment sections along the route was enhanced. However，the high-sensitivity transgenic cell line assay found that the effluent from both plants could lead to endoplasmic reticulum stress，which had potential ecological pollution risk. The result of fractionation analysis found that the ozone and activated carbon processes significantly reduced the toxicity of hydrophobic pollutants. Additionally，the results based on the fractionated samples were different from the original concentrated samples，indicating the combined toxic effects of pollutants were different under various mixing ratios. The types of toxic pollutants and the toxic mechanisms needed to be explored in depth. The results of this study provided basic data for the evaluation of biological toxicity and treatment efficiency of wastewater in industrial parks.

Key words: industrial wastewater, cytotoxicity, fractionation, HepG2 cell, Hela/CHOP cell

摘要：

工业废水组分复杂、污染严重，研究工业废水处理工艺的毒性削减能力对于水环境质量保障具有重要意义。融合污染物细胞毒性检测和分级分离方法，分析了2家典型工业园区污水处理厂沿程工艺段水样的毒性效应。结果表明，2座污水厂的进水都存在细胞增殖抑制、活性氧诱导、线粒体膜电位改变等毒性效应，出水中则不存在，且沿程部分处理工段水样毒性增强。但经高敏的转基因细胞株检测发现，2座污水厂出水都可诱导细胞内质网应激响应，具有潜在的生态污染风险。分级分离分析结果表明，臭氧与活性炭工艺显著降低了疏水性污染物的毒性；分馏样本的毒性检测结果与浓缩样本具有一定的差异性，表明污染物在不同混合比例下的联合毒性效应具有显著差异，相关致毒污染物类型及联合机理需深入分析。本研究可为工业园区废水的生物毒性效应及处理效率评估提供基础数据。

关键词: 工业废水, 细胞毒性, 分级分离, HepG2细胞, Hela/CHOP细胞

CLC Number:

X832
X703.1

Wanqing CHENG, Min JIA, Ling CHEN, Bing WU. Cytotoxicity reduction characteristics during wastewater treatment process in typical industrial park[J]. Industrial Water Treatment, 2022, 42(10): 65-70.

程婉清, 贾敏, 陈玲, 吴兵. 典型工业园区废水处理过程的细胞毒性削减特征[J]. 工业水处理, 2022, 42(10): 65-70.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2021-1280

https://www.iwt.cn/EN/Y2022/V42/I10/65

Figures/Tables 4

References 24

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