改进型ABR处理硫酸铵废水及微生物群落的影响

doi:10.19965/j.cnki.iwt.2020-1248

摘要/Abstract

摘要：

以改进型ABR系统对模拟硫酸铵废水进行处理研究，考察外加亚硝态氮源对污水中NH₄⁺-N和TN去除效果的影响，并对其中效果最好的n（NO₂^-）：n（NH₄⁺）为1：1时的各隔室NH₄⁺-N、TN变化进行分析。研究表明，外加亚硝态氮对改进型ABR中NH₄⁺-N和TN的去除有促进作用，n（NO₂^-）：n（NH₄⁺）为1：1时NH₄⁺-N和TN的平均去除率达到最高，分别为55.74%和69.16%。4个隔室的TN和NH₄⁺-N去除率之比分别为55：5：16：7和3：2：11：19。对不同条件下各隔室中的亚硝酸还原酶、羟胺氧化酶活性进行测定。结果显示在进水n（NO₂^-）：n（NH₄⁺）升高的同时，各隔室的酶活性也有显著上升。对n（NO₂^-）：n（NH₄⁺）为1：1时的各隔室中的污泥进行高通量分析，结果在1^#隔室中发现Enterobacter属的微生物，表明在1^#隔室中发生了好氧反硝化。

关键词: 改进型ABR, 硫酸铵废水, 外加亚硝态氮, 微生物群落分析, 硝化反硝化

Abstract:

The improved ABR system was used to treat simulated ammonium sulfate wastewater. The influence of additional nitrite nitrogen source on the removal effect of NH₄⁺-N and TN in sewage was investigated, and the changes of NH₄⁺-N and TN in each chamber when n(NO₂^-): n(NH₄⁺)=1:1 was analyzed. The results showed that the addition of nitrite could promote the removal of NH₄⁺-N and TN in the improve ABR. When n(NO₂^-): n(NH₄⁺)=1:1, the average removal rates of NH₄⁺-N and TN reached the highest values of 55.74% and 69.16%, respectively. The ratios of TN and NH₄⁺-N removal rates in four compartments were 55:5:16:7 and 3:2:11:19, respectively. The activities of nitrite reductase and hydroxylamine oxidase in each compartment under different conditions were determined. The results showed that when n(NO₂^-): n(NH₄⁺) of influent water increased, the enzyme activities in each compartment also increased significantly. High-throughput analysis was performed on the sludge in each compartment when n(NO₂^-): n(NH₄⁺)=1:1. According to the results, microorganisms of the genus of Enterobacter were found in compartment 1^#, indicating that aerobic denitrification occurred in compartment 1^#.

Key words: improved ABR, ammonium sulfate wastewater, plus nitrate nitrogen, microbial community analysis, nitration and denitrification

中图分类号:

X703.1

孙佳成,黄智,谢颖,农佳佳,朱姝,罗嫒玲,宿程远. 改进型ABR处理硫酸铵废水及微生物群落的影响[J]. 工业水处理, 2021, 41(9): 129-134.

Jiacheng Sun,Zhi Huang,Ying Xie,Jiajia Nong,Shu Zhu,Ailing Luo,Chengyuan Su. Effect of modified ABR on ammonium sulfate wastewater and microbial community[J]. Industrial Water Treatment, 2021, 41(9): 129-134.

https://www.iwt.cn/CN/Y2021/V41/I9/129

图/表 8

表1 改进型ABR系统进水条件

Table 1 Water inlet condition of modified ABR system

指标	COD/（mg·L^-1）	NH₄⁺-N/（mg·L^-1）	NO₂^--N/（mg·L^-1）	TN/（mg·L^-1）	pH
ABR进水	1 300~1 700	180~220	0~220	380~400	7.5~8
使用药剂	红糖	硫酸铵	亚硝酸钠	—	—
离子型稀土开采废水	60~80	180~220	0	380~400	4~6

图1 改进型ABR装置

Fig.1 Modified ABR equipment setup

表2 改进型ABR装置的运行条件

Table 2 Different operating conditions of modified ABR

指标	COD/（mg·L^-1）	n（NO₂^-）∶n（NH₄⁺）
条件1	1 300~1 700	0∶1
条件2	1 300~1 700	1∶0.5
条件3	1 300~1 700	1∶1

图2 不同n（NO₂^-）∶n（NH₄⁺）对污染物去除的影响

Fig.2 Effects of different n(NO₂^-)∶n(NH₄⁺) on pollutant removal

图3 n（NO₂^-）∶n（NH₄⁺）为1∶1时各隔室NH₄⁺-N、TN的变化情况

Fig.3 When n(NO₂^-)∶n(NH₄⁺) was 1∶1, the changes of ammonia nitrogen and total nitrogen in each compartment

图4 各隔室内的酶活性

Fig.4 Enzyme activity in each compartment

图5 门水平上各隔室中微生物群落分布情况

Fig.5 Distribution of microbial communities in different compartments at phylum level

图6 属水平上各隔室中微生物群落分布情况

Fig.6 Distribution of microbial community in each compartment at genus level

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