摘要：

采用分段进水多级AO工艺处理模拟酿造废水，通过水质检测和高通量测序技术分析0、30%、70%、100%四种原水比例进水模式下的水质处理效果和多级AO工艺中的微生物群落特征。结果表明，当进水COD、TP、NH₄⁺-N分别为635、33.22、63.75 mg/L时，随着进水原水比例的增加，受原水水质冲击反应器各出水指标去除效果均有所降低。高通量测序结果表明，门水平下四种进水模式的微生物组成大致相同，其中Proteobacteria、Patescibacteria和Actinobacteriota是该工艺的优势菌门；而Acidobacteriota是原水进水比例为0时独有的优势菌门。优势菌属为Candidatus-competibacte、Propionicicella、Terrimonas和Hyphomicrobium，大多是与硝化和反硝化过程有关的菌属，四种进水模式下，不同微生物菌群种类类似，但丰度差异较大。

关键词: 多级AO工艺, 酿造废水, 高通量测序, 微生物群落结构

Abstract:

Applying the multi-stage AO process to treat the simulated brewing water, analyze water quality treatment effect and microbial community characteristics in the multi-stage AO process of the four raw water proportion influent modes: 0, 30%, 70% and 100% based on the water quality detection and high-throughput sequencing technology. The result shows that when the influent COD, TP and NH4+-N concentrations are 635 mg/L, 33.22 mg/L and 63.75 mg/L, respectively, the removal efficiency of each effluent index in the reactor impacted by the raw water quality decreases with the increase of the influent raw water proportion. The high-throughput sequence shows that the microbial compositions of the four influent modes at the phylum level are roughly the same. Under this mode, the three kinds of bacteria, Proteobacteria, Patescibacteria and Actinobacteriota, are the dominant phyla of the process, while the Acidobacteriota is a unique dominant phylum when the proportion of raw water intake is 0. The dominant species are Candidatus-competibacte, Propionicicella, Terrimonas, and Hyphomicrobium, which are most related to nitrification and denitrification processes. In the four influent modes, different microbial communities are similar in species but different in abundance.

Key words: multistage AO process, brewing wastewater, high-throughput sequencing, microbial community structure

中图分类号: 

• X703