二乙二醇丁醚好氧污泥微生物群落结构及降解途径分析

doi:10.19965/j.cnki.iwt.2024-0400

摘要/Abstract

摘要：

采用好氧生物法对二乙二醇丁醚（DGBE）进行降解，过程中逐步提升进水DGBE浓度考察其降解效果。分别在驯化前、驯化稳定期和高浓度驯化期获取活性污泥样品进行高通量测序分析，并基于降解中间产物推测出DGBE可能存在的降解途径。实验结果表明：DGBE质量浓度低于832.85 mg/L时，DGBE和COD的去除率分别可达到99%和93%以上，继续提升进水浓度则去除率出现下降趋势。DGBE驯化后活性污泥微生物的丰富度和多样性大幅降低，驯化稳定期的优势菌门为Proteobacteria（78.59%），优势菌属为Cupriavidus（42.44%）和Rhodanobacter（20.02%），高浓度驯化期Actinobacteriota（51.15%）超越Proteobacteria（43.41%）成为丰度最高的菌门，Nakamurella（49.81%）为主要优势菌属。检测到DGBE的主要中间产物包括乙二醇丁醚、丁醛和乙烯基正丁醚，分析其可能的降解途径为ω-β-氧化降解途径和次末端氧化导致的醚键断裂降解途径。

关键词: 二乙二醇丁醚, 好氧降解, 微生物群落结构, 降解途径

Abstract:

Aerobic biodegradation was employed to degrade Diethylene glycol monobutyl ether(DGBE), and the degradation effect was investigated by gradually increasing the influent DGBE concentration. Activated sludge samples were collected for high-throughput sequencing analysis before, during the stable period, and at high concentration acclimation, respectively. The potential degradation pathways of DGBE were inferred based on the identification of intermediate products. The experimental results indicated that when the DGBE concentration was below 832.85 mg/L, the removal rates of DGBE and COD could achieve over 99% and 93%, respectively, while a further increase in influent concentration led to a decline in removal efficiency. The richness and diversity of the microbial community in the activated sludge were significantly reduced after DGBE acclimation. During the stable acclimation period, the dominant phylum was Proteobacteria(78.59%), with the dominant genera of Cupriavidus(42.44%) and Rhodanobacter(20.02%). In the high concentration acclimation period, Actinobacteriota(51.15%) surpassed Proteobacteria(43.41%) as the most abundant phylum, and Nakamurella(49.81%) was the primary dominant genus. The main intermediate products of DGBE were detected, as butyl glycol, butyraldehyde, and vinyl butyl ether, suggesting possible degradation pathways as the ω-oxidation and β-oxidation pathway, and the ether bond cleavage degradation pathway due to sub-terminal oxidation.

Key words: diethylene glycol monobutyl ether, aerobic degradation, microbial community structure, degradation pathways

中图分类号:

X703.1

关莹, 刘润, 秦品珠, 张瑞敏. 二乙二醇丁醚好氧污泥微生物群落结构及降解途径分析[J]. 工业水处理, 2024, 44(12): 86-93.

Ying GUAN, Run LIU, Pinzhu QIN, Ruimin ZHANG. Analysis of the aerobic sludge microbial community structure and degradation pathways of diethylene glycol monobutyl ether[J]. Industrial Water Treatment, 2024, 44(12): 86-93.

https://www.iwt.cn/CN/Y2024/V44/I12/86

图/表 8

图1 SBR实验装置

Fig.1 The diagram of SBR experimental device

图2 COD和DGBE进出水浓度、去除率和活性污泥MLSS和SV₃₀的变化

Fig.2 COD and DGBE concentrations and removal rates in the influent and effluent and variations of MLSS and SV₃₀ in activated sludge

图3 稀释曲线

Fig.3 Rarefaction curve

表1 活性污泥细菌群落Alpha多样性指数

Table 1 Alpha diversity index of activated sludge bacterial community

样品	ace	chao	shannon	simpson	coverage
S1	710.285	707.667	4.215	0.058	0.996
S2	174.089	177.059	2.372	0.209	0.999
S3	139.404	143.250	2.049	0.280	0.999

图4 微生物群落venn图

Fig.4 Venn diagram of microbial community

图5 门水平下的细菌群落分类分布

Fig.5 Bacterial communities at the phylum level

图6 属水平下的细菌群落分布

Fig.6 Bacterial communities at the genus level

图7 DGBE好氧生物降解途径

Fig.7 Aerobic biodegradation pathway of DGBE

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