生物蒸发处理冰淇淋生产废液的菌种筛选及应用研究

doi:10.19965/j.cnki.iwt.2021-1204

摘要/Abstract

摘要：

冰淇淋生产废液具有高有机物含量、高含水率、高黏度、易生物降解的特性，使得其处理处置难度大、成本高。通过微生物筛选实验，获得了3株形态各异的单菌株，接种不同菌种的对比实验表明，添加Pichia cecembensis的生物蒸发处理发酵温度最高、升温最快。进一步开展不同处理负荷的对比实验，结果表明，在处理负荷为1.5 kg/批的条件下，生物蒸发处理7 d，累积减量率达99.9%，含水率基本稳定在60%~65%的区间范围内，处理负荷提高至2.0 kg/批，需采取驯化培养或增加投菌量等手段来实现生物蒸发的连续稳定运行。生物蒸发去除的水分经冷凝回收后，COD降至600 mg/L以下，可有效降低后续废水的处理难度和处理成本。

关键词: 高浓度有机废液, 生物蒸发, 菌种筛选

Abstract:

Waste liquid from ice cream production has the characteristics of high organic content，high moisture content，high viscosity and easy biodegradation，which makes its treatment and disposal difficult and costly. Three single strains with different morphology were obtained by microbial screening experiment. The comparative experiment of inoculating different strains showed that the bio-evaporation treatment with Pichia cecembensis had the highest fermentation temperature and the fastest heating rate. Under the treatment load of 1.5 kg/batch，the reduction rates of bio-evaporation treatment for 7 days were 99.9%. The water content is basically stable in the range of 60%-65%. When the treatment load increased 2.0 kg/batch，the continuous and stable operation of biological evaporation needs to be realized by domesticating or incubating bacteria. After the water removed by bio-evaporation is condensed and recovered，the COD concentration was less than 600 mg/L，which can effectively reduce the difficulty and cost of subsequent wastewater treatment.

Key words: organic waste liquid with high concentration, bio-evaporation treatment, screening of bacteria

中图分类号:

X703.1

郑晓伟, 郭栋, 肖文敏, 王海潮, 刘丹. 生物蒸发处理冰淇淋生产废液的菌种筛选及应用研究[J]. 工业水处理, 2022, 42(9): 141-148.

Xiaowei ZHENG, Dong GUO, Wenmin XIAO, Haichao WANG, Dan LIU. Screening and application of bacteria for bio-evaporation process of waste liquid from ice cream production[J]. Industrial Water Treatment, 2022, 42(9): 141-148.

https://www.iwt.cn/CN/Y2022/V42/I9/141

图/表 7

表1 冰淇淋生产废液与木屑的物理、化学特性

Table 1 Physical and chemical properties of waste from ice cream production and sawdust

名称	含水率/%	密度/（g·mL^-1）	COD/（mg·L^-1）	总糖/（mg·L^-1）	pH
果冰废液	78.646±0.05	1.090±0.04	2.74×10⁵	3.13×10⁵	2.3~2.5
木屑	5.202±0.06	0.158±0.05	1.83×10⁵	—	7.2~7.5

图1 实验装置示意图

Fig. 1 Experimental device diagram

图2 3种菌株的菌落形态和革兰氏染色结果

Fig. 2 Colony morphology and gram staining of three strains

图3 接种不同菌种条件下生物蒸发发酵温度随时间的变化

Fig. 3 Changes of temperature in the process of bio-evaporation with different strains inoculated

图4 不同处理负荷条件下生物蒸发日减量率（a）和累积减量率（b）随时间的变化

Fig.4 Variation of daily reduction rate（a） and cumulative reduction rate（b） in the process of bio-evaporation

图5 不同处理负荷条件下生物蒸发发酵温度和含水率随发酵时间的变化

Fig.5 Variation of fermentation temperature and moisture content in the process of bio-evaporation

图6 不同处理负荷条件下的生物蒸发处理实验结果

Fig.6 Experimental results of biological evaporation treatment under different treatment loads

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