Abstract:

The phenomenon of microbial quorum sensing has been verified in wastewater treatment and applied in membrane fouling control，but it is rarely reported in the hybrid membrane bioreactor（HMBR）. The HMBR sewage treatment system was constructed and the HMBR equipment was operated under different sludge retention time（SRT=10，20，30 d）. The removal effect of pollutants by the HMBR sewage treatment system was investigated by analyzing the effluent quality. The community structure of quorum sensing related bacteria was detected by high-throughput sequencing，and the mechanism of quorum sensing regulating HMBR membrane fouling was analyzed in combination with transmembrane pressure difference and signal molecule concentration. The results showed that，the optimum working condition appeared under the condition of SRT=30 d，which improved the effluent quality and prolonged the membrane operation cycle. A variety of quorum quenching（QQ） bacteria were detected in HMBR，among them，Comamonas，Xanthomonadacea and Anaerolineaceae were relatively abundant. QQ bacteria had more obvious advantages than quorum sensing（QS） bacteria under long SRT，which helped to reduce the concentration of signal molecules in HMBR，hinder the progress of EPS secretion and optimize the operating cycles of the membrane module. Therefore，prolonging SRT to inhibit quorum induction was an effective method to regulate HMBR membrane fouling.

Key words: sludge retention time, membrane fouling, quorum sensing, signal molecule

摘要：

微生物群体感应现象已经在污水处理中被验证并应用于膜污染控制，但是其在复合式膜生物反应器（HMBR）中的膜污染控制研究鲜有报道。构建了HMBR污水处理系统，并在不同污泥龄（SRT=10、20、30 d）下运行HMBR设备，通过分析出水水质考察HMBR污水处理系统对污染物的去除效能；采用高通量测序检测群体感应相关菌群的群落结构，并结合跨膜压差、信号分子浓度深入解析群体感应调控HMBR膜污染的机理。实验结果表明，HMBR在SRT=30 d的工况下出水水质最好，膜组件运行周期最长，为最佳工况；HMBR内检测到多种群体感应猝灭（QQ）菌，其中从单胞菌科（Comamonas）、黄单胞菌科（Xanthomonadacea）和厌氧绳菌科（Anaerolineaceae）相对丰度较大；在长SRT下QQ菌比群体感应（QS）菌相对丰度更具优势，有助于降低HMBR内部信号分子的浓度，阻碍EPS分泌过程的正常进行，从而优化膜组件运行周期，因此通过延长SRT来抑制群体感应强度是一种有效的HMBR膜污染调控手段。

关键词: 污泥龄, 膜污染, 群体感应, 信号分子

CLC Number: 

• X703