Effect of SRT on EBPR system with sodium case in hydrolysate as carbon source

doi:10.19965/j.cnki.iwt.2022-0202

Abstract

Abstract:

In order to investigate the effect of sludge retention time（SRT） on the performance of biological phosphorus removal system，a sequential batch reactor（SBR） was used. In this system，sodium casein hydrolysate（Cas aa） was used as the only carbon source. At low temperature，the effects of SRT on the change of microbial community and the removal performance of organic matter were investigated. The results show that when the sludge retention time is 15 d，the system has the best phosphorus removal performance，and the phosphorus removal rate can reach more than 95%，which is significantly higher than other stages. The analysis of typical operation cycle of the system showed that when the sludge retention time was 15 d，the maximum anaerobic phosphorus release and aerobic phosphorus uptake of the system was 34.03 mg/L and 48.45 mg/L，respectively，and the phosphorus removal effect was the best. The results of high-throughput sequencing analysis showed that the composition and structure of polyphosphate accumulating organisms（PAOs） changed with the sludge retention time，and the relative abundance of all polyphosphate accumulating organisms was the highest when SRT was 15 d.

Key words: sludge retention time, sodium casein hydrolysate, enhanced biological phosphorus removal（EBPR）, polyphosphate accumulating organisms（PAOs）

摘要：

为了探究污泥龄（SRT）对以酪蛋白水解物（Cas aa）为唯一碳源的强化生物除磷（EPBR）系统性能的影响，采用序批式反应器（SBR），在低温条件下考察了SRT对Cas aa为碳源的EPBR中微生物菌群的变化及其污染物去除性能的影响。结果表明，SRT为15 d时，系统除磷性能最佳，除磷率可达95%以上，显著高于其他SRT条件。系统运行典型周期分析表明：当SRT为15 d时，系统的厌氧释磷质量浓度最大，为34.03 mg/L，好氧吸磷质量浓度最大，为48.45 mg/L，除磷效果最优。高通量测序分析结果表明，系统内聚磷菌（PAOs）的组成和结构会随着SRT的变化而变化，SRT为15 d时，系统内全体PAOs相对丰度最高。

关键词: 污泥龄, 酪蛋白水解物, 强化生物除磷, 聚磷菌

CLC Number:

X703.1

Chenzhu REN,Shaopo WANG,Lijie WANG,Yanmeng BI,Yajing LI,Jingjie YU. Effect of SRT on EBPR system with sodium case in hydrolysate as carbon source[J]. Industrial Water Treatment, 2023, 43(1): 56-61.

任晨竹,王少坡,王丽杰,毕艳孟,李亚静,于静洁. SRT对酪蛋白水解物为碳源的EPBR系统的影响[J]. 工业水处理, 2023, 43(1): 56-61.

/ / Recommend / Download Citations

URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2022-0202

https://www.iwt.cn/EN/Y2023/V43/I1/56

Figures/Tables 6

Fig. 1 The schematic diagram of the SBR used in this study

Fig.2 Change of PO₄^3- and COD concentration with time in EBPR system under different operating parameters

Fig.3 Changes of PO₄^3- and COD concentration with time in typical cycle of the system

Fig. 4 The concentration changes of PO₄^3-、Ploy-P and Gly during the typical period of the system

Fig. 5 Microbial community structure at phylum classification level in sludge with system SRT=15、20 and 10 days

Fig. 6 Comparison of the relative abundance of PAOs in sludge at different stages

References 15

1	IZADI P， IZADI P， ELDYASTI A，et al. A review of biochemical diversity and metabolic modeling of EBPR process under specific environmental conditions and carbon source availability［J］. Journal of Environmental Management，2021，288：112362． doi:10.1016/j.jenvman.2021.112362 doi: 10.1016/j.jenvman.2021.112362 URL
2	刘有华，王思婷，杨乔乔，等. 国内外水体富营养化现状及聚磷菌研究进展［J］. 江苏农业科学，2021，49（9）：26-35．
	LIU Youhua， WANG Siting， YANG Qiaoqiao，et al. Current situation of eutrophication of water body at home and abroad and research progress of phosphate accumulating bacteria［J］. Jiangsu Agricultural Sciences，2021，49（9）：26-35．
3	MIELCZAREK A T. Population dynamics of bacteria involved in enhanced biological phosphorus removal in Danish wastewater treatment plants［J］. Water Research，2013，47（4）：1529-1544． doi:10.1016/j.watres.2012.12.003 doi: 10.1016/j.watres.2012.12.003 URL
4	NGUYEN H T T， LE V Q， HANSEN A A，et al. High diversity and abundance of putative polyphosphate-accumulating Tetrasphaera-related bacteria in activated sludge systems［J］. FEMS Microbiology Ecology，2011，76（2）：256-267． doi:10.1111/j.1574-6941.2011.01049.x doi: 10.1111/j.1574-6941.2011.01049.x URL
5	郝晓地，陈峤，刘然彬. Tetrasphaera聚磷菌研究进展及其除磷能力辨析［J］. 环境科学学报，2020，40（3）：741-753．
	HAO Xiaodi， CHEN Qiao， LIU Ranbin. Research advances of Tetrasphaera as polyphosphate accumulating organisms and analysis on their P-removal potential［J］. Acta Scientiae Circumstantiae，2020，40（3）：741-753．
6	KRISTIANSEN R， NGUYEN H T T， SAUNDERS A M，et al. A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal［J］. The ISME Journal，2013，7（3）：543-554． doi:10.1038/ismej.2012.136 doi: 10.1038/ismej.2012.136 URL
7	SANTOS M M， LEMOS P C， REIS M A，et al. Glucose metabolism and kinetics of phosphorus removal by the fermentative bacterium Microlunatus phosphovorus ［J］. Applied and Environmental Microbiology，1999，65（9）：3920-3928． doi:10.1128/aem.65.9.3920-3928.1999 doi: 10.1128/aem.65.9.3920-3928.1999 URL
8	徐立杰，郭春艳，彭永臻，等. 强化生物除磷系统的微生物学及生化特性研究进展［J］. 应用与环境生物学报，2011，17（3）：427-434． doi:10.3724/sp.j.1145.2011.00427 doi: 10.3724/sp.j.1145.2011.00427 URL
	XU Lijie， GUO Chunyan， PENG Yongzhen，et al. Review on the microbiological and biochemical characteristics of enhanced biological phosphorus removal system［J］. Chinese Journal of Applied ＆ Environmental Biology，2011，17（3）：427-434． doi:10.3724/sp.j.1145.2011.00427 doi: 10.3724/sp.j.1145.2011.00427 URL
9	杨鹤，王少坡，张铁凡，等. 酪蛋白水解物碳源SBR生物除磷系统启动［J］. 工业水处理，2021，41（2）：52-57． URL
	YANG He， WANG Shaopo， ZHANG Tiefan，et al. Start-up of sodium casein hydrolysate carbon source SBR biological phosphorus removal system［J］. Industrial Water Treatment，2021，41（2）：52-57． URL
10	SMOLDERS G J， VAN DER MEIJ J， VAN LOOSDRECHT M C，et al. Model of the anaerobic metabolism of the biological phosphorus removal process：Stoichiometry and pH influence［J］. Biotechnology and Bioengineering，1994，43（6）：461-470． doi:10.1002/bit.260430605 doi: 10.1002/bit.260430605 URL
11	OEHMEN A， YUAN Z， BLACKALL L L，et al. Short-term effects of carbon source on the competition of polyphosphate accumulating organisms and glycogen accumulating organisms［J］. Water Science and Technology：A Journal of the International Association on Water Pollution Research，2004，50（10）：139-144． doi:10.2166/wst.2004.0629 doi: 10.2166/wst.2004.0629 URL
12	ACEVEDO B， CAMIA C， CORONA J E. The metabolic versatility of PAOs as an opportunity to obtain a highly P-enriched stream for further P-recovery［J］. Chemical Engineering Journal，2015，270：459-467． doi:10.1016/j.cej.2015.02.063 doi: 10.1016/j.cej.2015.02.063 URL
13	张华，马涛，李丽，等. 生物除磷工艺中高效聚磷菌的快速筛选和鉴定［J］. 安徽建筑大学学报，2020，28（1）：69-72．
	ZHANG Hua， MA Tao， LI Li，et al. Rapid screening and identification of efficient phosphorus accumulating organisms in biological phosphorus removal process［J］. Journal of Anhui Jianzhu University，2020，28（1）：69-72．
14	许秀红，李秀，李绍峰，等. 强化生物除磷系统中聚磷菌和聚糖菌的竞争研究进展［J］. 化学工程师，2017，31（1）：44-48． doi:10.16247/j.cnki.23-1171/tq.20170144 doi: 10.16247/j.cnki.23-1171/tq.20170144 URL
	XU Xiuhong， LI Xiu， LI Shaofeng，et al. Research developmenton the competition between PAO and GAO in enhanced biological phosphorus removal system［J］. Chemical Engineer，2017，31（1）：44-48． doi:10.16247/j.cnki.23-1171/tq.20170144 doi: 10.16247/j.cnki.23-1171/tq.20170144 URL
15	ROY S， GUANGLEI Q， ZUNIGA-MONTANEZ R. Recent advances in understanding the ecophysiology of enhanced biological phosphorus removal［J］. Current Opinion in Biotechnology，2021，67：166-174． doi:10.1016/j.copbio.2021.01.011 doi: 10.1016/j.copbio.2021.01.011 URL

[1]	Ying LI, Qiang LIU, Wei XIANG, Qiannan LI. Control of HMBR membrane fouling based on quorum sensing related bacteria analysis [J]. Industrial Water Treatment, 2022, 42(11): 122-126.
[2]	He Yang,Shaopo Wang,Tiefan Zhang,Jingjie Yu,Yajing Li,Chenchen Wang,Chunsheng Qiu,Liping Sun. Start-up of sodium casein hydrolysate carbon source SBR biological phosphorus removal system [J]. Industrial Water Treatment, 2021, 41(2): 52-57.
[3]	Liu Qiang, Liang Sen, Yan Junwei, Wang Hongbo, Song Qicheng. Influences of SRT on S-EPS and membrane fouling in a HMBR [J]. INDUSTRIAL WATER TREATMENT, 2019, 39(2): 34-37,41.
[4]	Li Wei, Wang Yujia, Zhu Lei, Xu Qiuhong. Effect of SRT on the denitrifying dephosphatation capacity of A/A process [J]. INDUSTRIAL WATER TREATMENT, 2015, 35(7): 55-59.
[5]	Li Bolin, Zhang Wenqin, Zhang Zhi, Li Ye, Song Mengting. Research on the influence of sludge running indexes on the biological denitrification by A/A/O oxidation ditch process [J]. INDUSTRIAL WATER TREATMENT, 2014, 34(5): 28-32.

Please choose a citation manager

Content to export