Abstract:

Multiple-tube fermentation technique , membrane-filter technique and enzyme substrate technique have been used respectively for the determination of total coliform,fecal coliform and Escherachaa cola in water. The pre-cision，consistency of testing results，etc. are compared. The optimum testing techniques for the three kinds of main bacteria have been chosen by the water quality testing laboratory. The results show that for the clean water sample detection , membrane-filter technique can be chosen as the first choice. For the detection of seriously polluted water samples，enzyme substrate technique can be chosen as the first choice.

Key words: multiple-tube fermentation technique, membrane-filter technique, enzyme substrate technique, total coliform, fecal coliform, Esc he ric hia c oli

摘要：

分别采用多管发酵法、滤膜法和酶底物法测定水中总大肠菌群、粪大肠菌群和大肠埃希氏菌,对3种方法的精密度、检测结果的一致性等进行了比较,总结得出水质检测实验室对此3个主要细菌学项目最佳的测定方法选择。结果表明,对清洁水样的检测可以选择滤膜法作为第一法,对重度污染水样的检测可以选择酶底物法作为第一法。

关键词: 多管发酵法, 滤膜法, 酶底物法, 总大肠菌群, 粪大肠菌群, 大肠埃希氏菌

CLC Number: 

• X703.1: X835