Abstract: The modified hydroxylamine oxidation method has been used for the determination of O₂^·- content in micro-organisms. Adding carbonate buffer solution whose pH=10.2 and NH₂OH solution 80 mmol/L to the system to be detected,after chromogenic reaction at 30℃ for 25 min,dichloromethane has been used as extraction agent,and then the absorbance A₅₃₀ in extraction phase detected by spectrometer. Combined with the factors,such as working curve of hydroxylamine oxidation method,sludge concentration,etc.,the O₂^·- content in microorganisms can be calculated. The method stated above is characterized by simple operation and high sensitivity. Therefore,it can be widely applied to the determination of O₂^·- in microorganism samples.

Key words: microorganisms, superoxide anion radicals, spectrophotography, hydroxylamine oxidization, dichlorome-thane

摘要： 采用改进的羟胺氧化法测试O₂^·-含量，在待测体系加入pH=10.2的碳酸缓冲溶液和80 mmol/L的NH₂OH溶液，于30℃下显色反应25 min后，以二氯甲烷为萃取剂并检测萃取相的吸光度A₅₃₀；再结合工作曲线、污泥浓度等因素，即可核算微生物体内O₂^·-含量。所述方法操作较为简便，灵敏度高，可在微生物样本O₂^·-测定方面得以推广与应用。

关键词: 微生物, 超氧阴离子自由基, 分光光度法, 羟胺氧化, 二氯甲烷

CLC Number: 

• X830