Nitrogen removal characteristics and application of bacterium Y5 tolerant to high concentration ammonia nitrogen

doi:10.19965/j.cnki.iwt.2023-0604

Abstract

Abstract:

Nitrogen removal from wastewater is one of the key steps in wastewater treatment. In order to remove high ammonia nitrogen content in water, Shigella boydii Y5, a strain with heterotrophic nitrification and aerobic denitrification performance, was screened from a chemical wastewater, and its optimal survival environment was investigated through single-factor experiments to examine its denitrification performance when applied to real wastewater. The results showed that the optimal environment was sucrose, C/N 10, pH 7, rotational speed 140 r/min, and the strain Y5 grown in this environment had high efficiency of denitrification in wastewater with initial mass concentration of 1 000 mg/L of ammonia nitrogen, and it was a salinophilic bacterium. When strain Y5 was applied to nucleic acid wastewater, the maximum degradation rates of ammonia nitrogen and total nitrogen reached 98.22% and 90.27%, respectively. High-throughput sequencing was used to analyze the stability and role of strain Y5 in the denitrification process of wastewater. The results demonstrated that there was a significant difference in the level of microbial diversity between the treatment group with the addition of carbon source and the treatment group with carbon source and strain Y5, suggesting that Shigella boydii Y5 had great application value for real wastewater treatment.

Key words: aerobic denitrification, Shigella boydii, denitrification performance, nucleic acid wastewater

摘要：

污水脱氮是污水净化处理的关键步骤之一。为去除水体中高浓度氨氮，于某化工污水中筛选出一株具有异养硝化-好氧反硝化性能的菌株鲍氏志贺菌（Shigella boydii Y5），通过单因素实验研究其最佳生存环境，并考察其应用于实际废水中的脱氮性能。结果表明，其最佳生存环境是碳源为蔗糖，C/N为10，pH为7，转速为140 r/min，此环境下生长的菌株Y5对初始质量浓度为1 000 mg/L氨氮废水具高效的脱氮能力，且菌株Y5为嗜盐菌。将菌株Y5应用于核酸废水，对氨氮和总氮的最大降解率达98.22%和90.27%。通过高通量测序分析废水脱氮过程中Y5菌株的作用和稳定性，结果表明，与只添加碳源处理组相比，添加碳源和菌株Y5处理组废水中微生物多样性水平出现显著性差异，表明Shigella boydii Y5在实际废水处理中有巨大应用价值。

关键词: 好氧反硝化, 鲍氏志贺菌, 脱氮特性, 核酸废水

CLC Number:

X703.1

Shiyuan LIU, Ting ZHANG, Yajuan GAO, Chenchen LI, Yongyu YANG, Guowei ZHANG, Qianqian TAN, Yongsheng JIN. Nitrogen removal characteristics and application of bacterium Y5 tolerant to high concentration ammonia nitrogen[J]. Industrial Water Treatment, 2024, 44(7): 162-170.

刘诗园, 张婷, 高雅娟, 李晨晨, 杨永宇, 张国伟, 谭骞骞, 靳永胜. 耐高浓度氨氮微生物Y5的脱氮特性及应用研究[J]. 工业水处理, 2024, 44(7): 162-170.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2023-0604

https://www.iwt.cn/EN/Y2024/V44/I7/162

Figures/Tables 12

Fig.1 Morphology of strain Y5

Fig.2 Phylogenetic tree of strain Y5

Fig.3 Effect of carbon source on the denitrification capacity of strain Y5

Fig.4 Effect of C/N on the denitrification capacity of strain Y5

Fig.5 Effect of pH on the denitrification capacity of strain Y5

Fig.6 Effect of rotational speed on the denitrification capacity of strain Y5

Fig.7 Effect of salt concentration on the denitrification capacity of strain Y5

Fig.8 Results of treatment of nucleic acid wastewater by strain Y5

Table 1 Wastewater quality

废水种类	pH	氨氮/（mg·L^-1）	总氮/（mg·L^-1）	COD/（mg·L^-1）
肝素钠废水	8.64	933.74	971.35	7 422.0
核酸废水	6.96	272.66	453.49	2 410.0

Table 2 α diversity index of two treatment groups

样本	Shannon	Simpson	Ace	Chao	Coverage/%
SUC	2.24	0.23	134.46	126.28	99.97
SUC+Y5	1.51	0.39	70.41	61.93	99.98

Fig.9 Relative abundance at the phylum level

Fig.10 Relative abundance at the genus level

References 27

1	沈桐，江进，李宁，等. 好氧反硝化细菌及其在微污染水源水修复中的应用研究进展［J］. 微生物学报，2023，63（2）：465-482.
	SHEN Tong， JIANG Jin， LI Ning，et al. Research progress of aerobic denitrifying bacteria and its application in remediation of slightly polluted source water［J］. Acta Microbiologica Sinica，2023，63（2）：465-482.
2	马咏琪，台喜生，王佳丽，等. 极端水质条件下异养硝化-好氧反硝化微生物的研究进展［J］. 微生物学通报，2024，51（3）：743-757.
	MA Yongqi， TAI Xisheng， WANG Jiali，et al. Research progress in heterotrophic nitrifying-aerobic denitrifying bacteria under extreme water quality conditions［J］. Microbiology China，2024，51（3）：743-757.
3	ZHANG Shusong， SUN Xingbin， FAN Yueting，et al. Heterotrophic nitrification and aerobic denitrification by Diaphorobacter polyhydroxybutyrativorans SL-205 using poly（3-hydroxybutyrate-co-3-hydroxyvalerate） as the sole carbon source［J］. Bioresource Technology，2017，241：500-507. doi:10.1016/j.biortech.2017.05.185
4	ROBERTSON L A， KUENEN J G. Aerobic denitrification：Old wine in new bottles？［J］. Antonie Van Leeuwenhoek，1984，50（5）：525-544. doi:10.1007/bf02386224
5	沈雯婷，邱田娟，何亦，等. 一株好氧反硝化菌脱氮性能研究［J］. 广东化工，2022，49（14）：41-43. doi:10.3969/j.issn.1007-1865.2022.14.014
	SHEN Wenting， QIU Tianjuan， HE Yi，et al. Study on the denitrification performance of an aerobic denitrifying strain of bacteria［J］. Guangdong Chemical Industry，2022，49（14）：41-43. doi:10.3969/j.issn.1007-1865.2022.14.014
6	杨景瑞，王莹，陈虎，等. 一株好氧反硝化菌的脱氮特性及其氮代谢机理研究［J］. 工业水处理，2023，43（10）：135-141. doi:10.19965/j.cnki.iwt.2023-0192
	YANG Jingrui， WANG Ying， CHEN Hu，et al. Nitrogen removal characteristics and metabolism mechanism of an aerobic denitrifying bacteria［J］. Industrial Water Treatment，2023，43（10）：135-141. doi:10.19965/j.cnki.iwt.2023-0192
7	陈思宇，刘晶，杨正，等. 低温异养硝化-好氧反硝化菌的分离及其除氮特性［J］. 湖南农业大学学报：自然科学版，2022，48（6）：712-717.
	CHEN Siyu， LIU Jing， YANG Zheng，et al. Isolation and nitrogen removal characteristics of a low temperature heterotrophic nitrification-aerobic denitrification bacterium［J］. Journal of Hunan Agricultural University（Natural Sciences），2022，48（6）：712-717.
8	张笑迎. 高氮废水脱氮微生物菌群的脱氮特性及微生物多样性分析［D］. 武汉：武汉轻工大学，2022. doi:10.3390/microorganisms10020235
	ZHANG Xiaoying. Nitrogen removal characteristics and microbial diversity analysis of denitrifying microorganisms in high nitrogen wastewater［D］. Wuhan：Wuhan Polytechnic University，2022. doi:10.3390/microorganisms10020235
9	HJ 636—2012 水质总氮的测定碱性过硫酸钾消解紫外分光光度法［S］. doi:10.12173/j.issn.1004-5511.202302011
10	HJ 535—2009 水质氨氮的测定纳氏试剂分光光度法［S］. doi:10.12173/j.issn.1004-5511.202111004
11	CHEN Mengping， HE Tengxia， WU Qifeng，et al. Enhanced heterotrophic nitrification and aerobic denitrification performance of Glutamicibacter arilaitensis EM-H8 with different carbon sources［J］. Chemosphere，2023，323：138266. doi:10.1016/j.chemosphere.2023.138266
12	夏远舰，杨小丽，李海华，等. 异养硝化-好氧反硝化菌Acinetobacter johnsonii sp. N26的脱氮性能及代谢途径［J］. 微生物学通报，2023，50（4）：1374-1395.
	XIA Yuanjian， YANG Xiaoli， LI Haihua，et al. Optimization of nitrogen removal performance and metabolic pathway of a heterotrophic nitrifying-aerobic denitrifying bacterial strain Acinetobacter johnsonii sp. N26［J］. Microbiology China，2023，50（4）：1374-1395.
13	陈猛，李安章，张明霞，等. 一株异养硝化-好氧反硝化菌的分离鉴定及其对养殖废水中氮的去除特性［J］. 农业资源与环境学报，2020，37（2）：270-279. doi:10.13254/j.jare.2019.0059
	CHEN Meng， LI Anzhang， ZHANG Mingxia，et al. Isolation and identification of a heterotrophic nitrifying and aerobic denitrifying strain and its removal characteristics of nitrogen in breeding wastewater［J］. Journal of Agricultural Resources and Environment，2020，37（2）：270-279. doi:10.13254/j.jare.2019.0059
14	DENG Min， ZHAO Xiaoli， SENBATI Y，et al. Nitrogen removal by heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas sp. DM02：Removal performance，mechanism and immobilized application for real aquaculture wastewater treatment［J］. Bioresource Technology，2021，322：124555. doi:10.1016/j.biortech.2020.124555
15	张宇红，刘香宇，董先博，等. 好氧反硝化细菌SY-D-22的分离、优化及脱氮机理［J］. 微生物学通报，2023，50（5）：1815-1825.
	ZHANG Yuhong， LIU Xiangyu， DONG Xianbo，et al. Aerobic denitrifying bacterium SY-D-22：Isolation，optimization of denitrification conditions，and characterization of denitrification mechanism［J］. Microbiology China，2023，50（5）：1815-1825.
16	邹玉兰，王莹，陈虎，等. 恶劣环境下异养硝化-好氧反硝化菌的研究进展［J］. 工业水处理，2020，40（12）：8-13. doi:10.11894/iwt.2020-0040
	ZOU Yulan， WANG Ying， CHEN Hu，et al. Research progress of heterotrophic nitrification-aerobic denitrification bacteria in harsh environment［J］. Industrial Water Treatment，2020，40（12）：8-13. doi:10.11894/iwt.2020-0040
17	王贺，衣萌萌，王淼，等. 一株高效好氧反硝化菌DS2的分离鉴定及脱氮特性［J］. 农业生物技术学报，2022，30（10）：1997-2008. doi:10.3969/j.issn.1674-7968.2022.10.014
	WANG He， YI Mengmeng， WANG Miao，et al. Isolation，identification，denitrification characteristics of a highly efficient aerobic denitrifying bacterial strain DS2［J］. Journal of Agricultural Biotechnology，2022，30（10）：1997-2008. doi:10.3969/j.issn.1674-7968.2022.10.014
18	WEI Bohui， LUO Xiao， MA Wenkai，et al. Biological nitrogen removal and metabolic characteristics of a novel cold-resistant heterotrophic nitrification and aerobic denitrification Rhizobium sp. WS7［J］. Bioresource Technology，2022，362：127756. doi:10.1016/j.biortech.2022.127756
19	LEE K Y， KIM K W， PARK M，et al. Novel application of nanozeolite for radioactive cesium removal from high-salt wastewater［J］. Water Research，2016，95：134-141. doi:10.1016/j.watres.2016.02.052
20	刘爱民. 嗜盐菌的研究进展［J］. 安徽师范大学学报（自然科学版），2002，25（2）：181-184. doi:10.3969/j.issn.1001-2443.2002.02.024
	LIU Aimin. Study advance on the halophile［J］. Journal of AnHui normal University（Natural Sciences），2002，25（2）：181-184. doi:10.3969/j.issn.1001-2443.2002.02.024
21	云玉攀. Pd-Cu催化剂协同零价铁处理污水厂二级出水中硝态氮的研究［D］. 北京：北京科技大学，2018.
	YUN Yupan. Study on treatment of nitrate nitrogen in secondary effluent of sewage plant with Pd-Cu catalyst and zero-valent iron［D］. Beijing：University of Science and Technology Beijing，2018.
22	田润敏，赵铎，洪艳华，等. 龙凤湿地与珰奈湿地底泥细菌古菌多样性及其对环境因子的响应［J］. 微生物学通报，2022，49（7）：2500-2514.
	TIAN Runmin， ZHAO Duo， HONG Yanhua，et al. Diversity of bacteria and Archaea in sediments of Longfeng wetland and Dangnai wetland and its response to environmental factors［J］. Microbiology China，2022，49（7）：2500-2514.
23	陈春秀，马超，刘皓，等. 一株异养硝化-好氧反硝化菌株的分离鉴定及其对养殖废水的脱氮效果［J］. 河北渔业，2022（10）：8-12. doi:10.3969/j.issn.1004-6755.2022.10.003
	CHEN Chunxiu， MA Chao， LIU Hao，et al. Isolation and identification of a heterotrophic nitrification-aerobic denitrification strain and its denitrification effect on aquaculture wastewater［J］. Hebei Fisheries，2022（10）：8-12. doi:10.3969/j.issn.1004-6755.2022.10.003
24	LU Huijie， CHANDRAN K， STENSEL D. Microbial ecology of denitrification in biological wastewater treatment［J］. Water Research，2014，64：237-254. doi:10.1016/j.watres.2014.06.042
25	CAO Shenbin， DU Rui， LI Baikun，et al. High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater［J］. Applied Microbiology and Biotechnology，2016，100（14）：6457-6467. doi:10.1007/s00253-016-7427-6
26	王欣晔. 耐冷好氧反硝化菌的筛选与脱氮效果研究［D］. 保定：河北大学，2022.
	WANG Xinye. Screening of cold-tolerant aerobic denitrifying bacteria and study on nitrogen removal effect［D］. Baoding：Hebei University，2022.
27	郭丽芸，时飞，杨柳燕，等. 好氧反硝化菌处理低浓度含氮污水［J］. 江苏农业科学，2016，44（8）：523-526.
	GUO Liyun， SHI Fei， YANG Liuyan，et al. Treatment of low concentration nitrogen-containing wastewater by aerobic denitrifying bacteria［J］. Jiangsu Agricultural Sciences，2016，44（8）：523-526.

[1]	Yueming ZHOU, Yi LIU, Li LIU, Yu SHEN, Maoqiu XIANG. Characteristics of HN-AD microbiota and its application in the treatment of aquaculture wastewater [J]. Industrial Water Treatment, 2024, 44(10): 1-17.
[2]	Jingrui YANG, Ying WANG, Hu CHEN, Yongkang LÜ. Nitrogen removal characteristics and metabolism mechanism of an aerobic denitrifying bacteria [J]. Industrial Water Treatment, 2023, 43(10): 135-141.
[3]	Yulan Zou,Ying Wang,Hu Chen,Yongkang Lü. Research progress of heterotrophic nitrification-aerobic denitrification bacteria in harsh environment [J]. Industrial Water Treatment, 2020, 40(12): 8-13.
[4]	Zhiqing Zhao,Le Huang,Weiyi Qiu,Shuwen Shen,Ping Xiong. Simultaneous aniline degradation and aerobic denitrification characteristics of an aerobic denitrifier [J]. Industrial Water Treatment, 2019, 39(7): 14-18.
[5]	Gong Youkui, Li Yongbo, Peng Yongzhen. Start-up and N₂O emission of the nitrosation reactor in different aeration modes [J]. INDUSTRIAL WATER TREATMENT, 2019, 39(4): 21-24,57.
[6]	Deng Xiongwen, Wang Han, Li Hanxiang, Zeng Qiansong, Fang Fang, Guo Jinsong. Denitrification influences of nitrogen loading under salinity condition on the ANAMMOX-EGSB reactor [J]. INDUSTRIAL WATER TREATMENT, 2018, 38(5): 35-39.
[7]	Wei Hao, Lü Hong, Zhou Jiti, Wang Jing, Zhang Qian, Zhou Yang. Research on the characteristics of bisphenol F degradation and denitrification by Pseudomonas sp. HS-2 [J]. INDUSTRIAL WATER TREATMENT, 2018, 38(1): 52-56.
[8]	He Huan, Yu Xuan, Han Yatao, Zhan Di, Shi Kaiyi. Research progress in the denitrification characteristics of heterotrophic nitrification-aerobic denitrification bacteria [J]. INDUSTRIAL WATER TREATMENT, 2017, 37(4): 12-17.
[9]	Weng Zihang, Lü Hong, Zhou Jiti. Research progress in the denitrification performances of heterotrophic nitrifying-aerobic denitrifying bacteria [J]. INDUSTRIAL WATER TREATMENT, 2017, 37(3): 21-25.
[10]	Wang Yonggang, Wang Xu, Zhang Jun'e, Dong Jing, Zhang Nan, Li Mingwei. Progress in the research and application of aerobic denitrification bacteria [J]. INDUSTRIAL WATER TREATMENT, 2017, 37(2): 12-17.
[11]	Pan Menglan, Huang Shaobin, Yi Li, He Jiaxin. Research on the denitrification capacity of aerobic denitrifier for sulfate containing wastewater [J]. INDUSTRIAL WATER TREATMENT, 2017, 37(1): 73-75,78.
[12]	Su Wanyun, Gao Junfa, Zhao Hongmei. Research progress in heterotrophic nitrification-aerobic denitrification bacteria [J]. INDUSTRIAL WATER TREATMENT, 2013, 33(12): 1-5.
[13]	Wu Wenqing, Huang Shaobin, Zhang Ruifeng, Xiao Xiannian. Study on the influencing factors of nitrogen removal using the biological aerated filter under the condition with mercury ions [J]. INDUSTRIAL WATER TREATMENT, 2012, 32(6): 39-42.
[14]	Han Xiaogang, Chen Ying, Yan Wenyao. Design of the nucleic acid wastewater treatment process [J]. INDUSTRIAL WATER TREATMENT, 2008, 28(10): 77-79.
[15]	Liu Jun, Wang Bin, Pan Deng, Li Siqing, Wang Ping. Study on the denitrification route in aerobic denitrification process [J]. INDUSTRIAL WATER TREATMENT, 2003, 23(11): 53-56.

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