Sludge adaptability during start-up of anaerobic reactor for leachate from garbage power plant

doi:10.19965/j.cnki.iwt.2023-0955

Abstract

Abstract:

Suitable anaerobic sludge can effectively shorten the start-up time of anaerobic reactors. To reveal the adaptability of different sources of anaerobic sludge to leachate from garbage power plants, the treatment performance of five types of anaerobic sludge on leachate from two different sources was investigated through intermittent cultivation. Illumina Miseq sequencing was used to reveal the main functional variability of different anaerobic sludge from the perspective of sludge community composition. The results showed that for leachate with COD up to 30 000 mg/L, inoculation with anaerobic sludge from a paper mill (ZZC sludge) resulted in a COD removal rate of 72.3% within 36 hours, and the sludge load (COD load of per unit MLVSS) reached 10.2 kg/(kg·d). For the leachate with a COD of 25 000 mg/L, the COD removal rate of ZZC sludge reached 75.8% after 36 hours, and the sludge load reached 10.1 kg/(kg·d). When the COD was 30 000 mg/L, ZZC sludge had better treatment effect on both eastern(DB) and southern(NB) plant leachate, NJG sludge (from agricultural processing plant) had the best treatment effect on DB plant leachate, and YZC sludge (from aquaculture wastewater treatment plant) had the best treatment effect on NB plant leachate. The rapid start-up of anaerobic reactors in two plant areas using ZZC sludge could effectively improve the methane production of anaerobic reactors.

Key words: sludge inoculation, anaerobic sludge, landfill leachate, microbial community characteristics, methanogenic bacteria

摘要：

适合的厌氧污泥可有效缩短厌氧反应器启动时间。为揭示不同来源厌氧污泥对垃圾发电厂渗滤液的适应性，通过间歇培养方式考察了5种厌氧污泥对两种不同来源垃圾渗滤液的处理性能，并通过Illumina Miseq测序从污泥群落组成角度揭示不同厌氧污泥主要功能差异性。结果表明：对于COD高达30 000 mg/L的渗滤液，接种造纸厂厌氧污泥（ZZC污泥），COD去除率在36 h达到72.3%，污泥负荷（单位质量MLVSS的COD负荷）达到10.2 kg/（kg·d）；对于COD为25 000 mg/L的渗滤液，接种ZZC污泥COD去除率36 h达到75.8%，污泥负荷达到10.1 kg/（kg·d）。当进水COD为30 000 mg/L时，ZZC污泥对东部（DB）和南部（NB）厂区渗滤液的处理效果均较好，农加工污泥（NJG污泥）对DB厂区渗滤液的处理效果最好，养殖厂污泥（YZC污泥）对NB厂区渗滤液的处理效果最好。使用ZZC污泥进行两厂区厌氧反应器的快速启动，可以有效提高厌氧反应器的甲烷产量。

关键词: 接种污泥, 厌氧污泥, 垃圾渗滤液, 微生物群落特性, 产甲烷菌

CLC Number:

X703

Likun GU, Jianxin HAO, Jinwei YUE, Heming XU, Donghui CAO, Yichao LI, Zhaoxu PENG, Jianyun ZHANG. Sludge adaptability during start-up of anaerobic reactor for leachate from garbage power plant[J]. Industrial Water Treatment, 2024, 44(9): 75-84.

谷立坤, 郝建新, 岳金葳, 许贺铭, 曹冬辉, 李奕潮, 彭赵旭, 张建云. 垃圾发电厂渗滤液厌氧反应器启动污泥适应性研究[J]. 工业水处理, 2024, 44(9): 75-84.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2023-0955

https://www.iwt.cn/EN/Y2024/V44/I9/75

Figures/Tables 13

Table 1 MLSS and MLVSS of different anaerobic sludge

污泥种类	YZC	NJG	ZZC	FSC-1	FSC-2
MLSS/（g·L^-1）	8.3	8.5	7.9	10.3	8.7
MLVSS/（g·L^-1）	6.1	6.1	5.1	7.7	6.6

Fig.1 Particle size distribution of inoculated sludge

Table 2 Water quality of leachate

渗滤液	pH	COD/（mg·L^-1）	NH₄ ⁺-N/（mg·L^-1）	NO₃ ^--N/（mg·L^-1）	NO₂ ^--N/（mg·L^-1）	TN/（mg·L^-1）	TP/（mg·L^-1）
东部厂区（DB）	5.75	55 200	786.91	15.86	9.35	1 597.25	15.77
南部厂区（NB）	5.91	53 600	724.86	56.36	1.84	1 477.06	15.22

Fig.2 Treatment effects of anaerobic sludge on leachate of different COD

Fig.3 Changes of sludge load

Table 3 Analysis of diversity and abundance of microbial community

污泥种类	OTUs	Shannon	ACE	Chao1	Simpson	Coverage
YZC	1 091	1.75	1 391	1 391	0.036	1.00
NJG	957	1.66	1 218	1 218	0.047	1.00
ZZC	1 687	2.24	2 423	2 423	0.011	1.00
FSC-1	1 443	2.01	2 021	2 021	0.018	1.00
FSC-2	1 129	1.64	1 386	1 386	0.052	1.00

Fig.4 Venn diagram of species abundance clustering of anaerobic sludge

Fig.5 Microbial community of anaerobic sludge at phylum level

Fig.6 Microbial community of anaerobic sludge at genus level

Fig.7 Abundance of methanogenic bacteria species

Fig.8 Abundance of hydrolytic acidifying bacteria species

Fig.9 Heat map of relative abundance of methane production related functional genes

Fig.10 Sludge operation effect

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