工业水处理 ›› 2019, Vol. 39 ›› Issue (7): 14-18. doi: 10.11894/iwt.2018-0557

• 试验研究 • 上一篇    下一篇

一株好氧反硝化菌的同步脱氮降解苯胺特性

赵芝清1,2(),黄乐3,邱唯一1,沈舒雯1,熊萍1   

  1. 1. 衢州学院化学与材料工程学院, 浙江衢州 324000
    2. 浙江大学环境与资源学院, 浙江杭州 310018
    3. 浙江工商大学环境科学与工程学院, 浙江杭州 310018
  • 收稿日期:2019-03-22 出版日期:2019-07-20 发布日期:2019-08-07
  • 作者简介:赵芝清(1979-),博士,副教授。电话:13819014805, E-mail:zhaozhiqing35@163.com
  • 基金资助:
    国家自然科学基金项目(21607092);衢州市科技局资助项目(2015Y012);衢州学院中青年学术骨干项目(XNZQN201506);衢州学院博士科研启动项目(BSJX201601);省级大学生科技创新活动计划暨新苗人才计划(2017R429003)

Simultaneous aniline degradation and aerobic denitrification characteristics of an aerobic denitrifier

Zhiqing Zhao1,2(),Le Huang3,Weiyi Qiu1,Shuwen Shen1,Ping Xiong1   

  1. 1. College of Chemical & Material Engineering, Quzhou University, Quzhou 324000, China
    2. College of Environment & Resource Sciences, Zhejiang University, Hangzhou 310018, China
    3. School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, China
  • Received:2019-03-22 Online:2019-07-20 Published:2019-08-07
  • Supported by:
    国家自然科学基金项目(21607092);衢州市科技局资助项目(2015Y012);衢州学院中青年学术骨干项目(XNZQN201506);衢州学院博士科研启动项目(BSJX201601);省级大学生科技创新活动计划暨新苗人才计划(2017R429003)

摘要:

采用批式实验探究了碳源、抗生素、重金属对好氧反硝化苯胺降解菌株同步脱氮降解苯胺特性的影响。结果表明:经生理生化反应和16S rDNA测序,鉴定为不动杆菌属(命名为Acinetobacter sp.H3)。在30℃、90 r/min振荡培养条件下,异养硝化率、TN去除率、苯胺降解率最大分别达57.98%、54.24%、100%。在35 μg/L抗生素和80 mg/L重金属胁迫下,苯胺仍能完全转化降解,好氧反硝化则受到了不同程度的抑制。

关键词: Acinetobacter sp.H3, 好氧反硝化, 苯胺降解

Abstract:

Batch experiments were conducted to investigate the effects of carbon source, antibiotics and heavy metals on the characteristics of the simultaneous aerobic denitrification and aniline degradation of an aerobic denitrifier. The results showed that it was identified as a member of the genus Acinetobacter (named Acinetobacter sp. H3) by physiological and biochemical reaction and its 16S rDNA gene sequence analysis. Under the shaking culture conditions of 30℃ and 90 r/min, the heterotrophic nitrification rate, TN removal rate and aniline degradation rate reached 57.98%, 54.24% and 100%, respectively. Under the stress of 35 μg/L antibiotics and 80 mg/L heavy metals, aniline could still be completely transformed and degraded, and aerobic denitrification was inhibited to varying degrees.

Key words: Acinetobacter sp. H3, aerobic denitrification, aniline degradation

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