摘要： Pseudomonas sp． HS-2能以双酚F为唯一碳源、NH₄⁺-N为唯一氮源进行生长，并通过异养硝化好氧反硝化过程脱氮。在35℃，150 r/min和pH=7.0条件下，考察了菌株HS-2降解双酚F的能力及其脱氮性能。结果表明，菌株HS-2能够降解500 mg/L双酚F。并且在最适碳氮比为20的条件下，双酚F和NH₄⁺-N的去除率可分别达到99%和96%。并且发现氨单加氧酶参与了双酚F的降解。氮平衡分析表明，47.7%的初始NH₄⁺-N转化为N₂。外加柠檬酸钠或硝态氮对双酚F和NH₄⁺-N的去除有明显的促进作用。

关键词: 双酚F降解, 脱氮, 异养硝化好氧反硝化, Pseudomonas sp. HS-2

Abstract: Pseudomonas sp. HS-2 can grow by using bisphenol F as sole carbon source and NH₄⁺-N as sole nitrogen source, and denitrify via the process of heterotrophic nitrification-aerobic denitrification(HN-AD). Bisphenol F de-gradation and denitrification capabilities of strain HS-2 have been investigated under the following conditions:35℃, 150 r/min and pH 7.0. The results show that strain HS-2 could efficiently degrade 500 mg/L of bisphenol F. Under the optimal C/N ratio of 20, the removing rates of bisphenol F and NH₄⁺-N can reach 99% and 96% in 48 h, respectively. In addition, it is found that during this process, ammonia monooxygenase has participated in BPF degradation. Nitrogen balance analysis demonstrates that 47.7% of initial NH₄⁺-N is converted into N₂. The additional sodium citrate and NO₃^--N have remarkable facilitating effects on the removal of bisphenol F and NH₄⁺-N.

Key words: bisphenol F degradation, denitrification, heterotrophic nitrification-aerobic denitrification, Pseudomonas sp.HS-2

中图分类号: 

• X703.1