摘要：

以咖啡果胶水解液作为外加碳源，通过分析不同HRT和m（COD）/m（TN）（记作C/N）下反硝化生物滤池（DNBF）的脱氮效果和反应器中微生物的群落特征，探究了咖啡果胶水解液的脱氮性能，并将其与传统碳源乙酸钠进行比较。研究结果表明，咖啡果胶水解液系统的最佳HRT=1 h，最佳C/N为6，在此条件下，当进水TN、NH₄ ⁺-N分别为（33.97±2.06）、（3.09±0.19） mg/L时，咖啡果胶水解液系统可将出水TN和NH₄ ⁺-N分别降至（3.12±0.59） mg/L和（2.19±0.14） mg/L，出水NO₃ ^--N稳定在1 mg/L以下，NO₃ ^--N去除率达到97.13%，NO₂ ^--N无明显累积，单位质量COD反硝化去除的NO₃ ^--N为0.22 g/g，接近于对照组乙酸钠的反硝化能力。咖啡果胶水解液系统中的微生物多样性优于乙酸钠体系，Zoogloea、Ignavibacterium、unclassified_Prolixibacteraceae、Longilinea等降解有机物的功能微生物和反硝化功能微生物大量存在，保证了咖啡果胶水解液作为碳源的反硝化效果。

关键词: 反硝化, 碳源, 生物脱氮, 咖啡果胶

Abstract:

Coffee pectin hydrolysate as an additional carbon source, the denitrification performance was explored by analyzing the denitrification effect in denitrifying biofilter(DNBF) reactor under different HRT and m(COD)/m(TN) (C/N) and microbial community characteristics, and compared with the traditional carbon source of sodium acetate. The results showed that the optimal HRT for the coffee pectin hydrolysate system was 1 h, and the optimal C/N was 6. Under these conditions, when the influent TN and NH₄ ⁺-N were (33.97±2.06) mg/L and (3.09±0.19) mg/L respectively, coffee pectin hydrolysate as carbon source could reduce the effluent TN and NH₄ ⁺-N to (3.12±0.59) mg/L and(2.19±0.14) mg/L, respectively. The effluent NO₃ ^--N was stabilized below 1 mg/L, and the removal rate reached 97.13%. NO₂ ^--N had no obvious accumulation. The NO₃ ^--N removed by denitrification per unit COD was 0.22 g/g, which was close to that of the control group sodium acetate. The microbial diversity in the system of coffee pectin hydrolysate was better than that of sodium acetate. There were a large quantities of microorganisms with organic degradation function and denitrification function, such as Zoogloea, Ignavibacterium, unclassified_prolixibacitraceae and Longilinea, which ensured the denitrification effect of coffee pectin hydrolysate as a carbon source.

Key words: denitrification, carbon source, biological denitrification, coffee pectin

中图分类号: 

• X703.1