氢自养还原菌同步降解水中4-溴酚及硝酸盐

doi:10.19965/j.cnki.iwt.2022-0851

摘要/Abstract

摘要：

基于摇瓶研究了以H₂为电子供体的氢自养还原菌降解4-溴酚（4-BP）的可行性；并在此基础上研究了4-BP浓度、硝酸盐（NO₃ ^--N）浓度及氢分压对4-BP的去除影响；最后研究了4-BP加入前后的微生物菌群变化。结果表明，提高初始4-BP浓度会降低其去除效果；作为一种优先电子受体，NO₃ ^--N会与4-BP争夺电子供体H₂，增大初始NO₃ ^--N浓度导致4-BP去除效果下降；提高氢分压会使得单位浓度NO₃ ^--N及4-BP获得更多的电子供体，提升两者还原速率；加入4-BP后，改变了微生物的生长环境，使得具有脱卤能力的Thauera丰度提升，达到42.8%。当系统中存在H₂时，具有正常活性的氢自养还原菌，在脱卤酶作用下4-BP释放Br^-从而被降解。

关键词: 氢自养还原菌, 4-溴酚, 同步降解

Abstract:

The feasibility of degrading 4-bromophenol by hydrogen autotrophic reducing bacteria using H₂ as electron donor was studied based on shake flasks. On this basis，the effects of 4-BP concentration，nitrate concentration and hydrogen partial pressure on the removal of 4-BP were studied. Finally，the microbial flora changes before and after the addition of 4-BP were investigated. The results showed that increasing the initial 4-BP concentration could reduce its removal effect. As a preferential electron acceptor，nitrate will compete with 4-BP for electron donor H₂，and increasing the initial nitrate concentration leads to a decrease in the removal efficiency of 4-BP. Increasing the hydrogen partial pressure could make nitrate and 4-BP obtain more electron donors per unit concentration，and increase the reduction rate of both. After adding 4-BP，the growth environment of microorganisms changed，and the abundance of Thauera with dehalogenation ability increased to 42.8%. When there existed H₂ in the system，the hydrogen autotrophic reducing bacteria with normal activity could release Br^- under the action of dehalogenase and be degraded.

Key words: hydrogen autotrophic reducing bacteria, 4-bromophenol, synchronous degradation

中图分类号:

X703.1

陈博, 丁婉莹, 张毅, 范玉荣, 宋玉峰, 李海翔. 氢自养还原菌同步降解水中4-溴酚及硝酸盐[J]. 工业水处理, 2023, 43(8): 82-88.

Bo CHEN, Wanying DING, Yi ZHANG, Yurong FAN, Yufeng SONG, Haixiang LI. Synchronous degradation of 4-BP and nitrate in water by hydrogen autotrophic reducing bacteria[J]. Industrial Water Treatment, 2023, 43(8): 82-88.

https://www.iwt.cn/CN/Y2023/V43/I8/82

图/表 10

表1 实验配水成分

Table 1 Experimental water composition

成分	质量浓度/（mg·L^-1）	成分	质量浓度/（mg·L^-1）
KNO₃（以N计）	10	ZnSO₄·7H₂O	0.1
Na₂SO₄（以SO₄ ^2-）	55	H₃BO₃	0.3
NaHCO₃	80	CoCl₂·6H₂O	0.2
Na₂HPO₄	216	CuCl₂·2H₂O	0.01
KH₂PO₄	236	Na₂MoO₄·2H₂O	0.03
FeSO₄·7H₂O	1.0	MnCl₂	0.01
CaCl₂	1.0	NiCl₂·6H₂O	0.01

表2 可行性实验设计

Table 2 Feasibility test design

项目		NO₃ ^--N/（mg·L^-1）	4-BP/ （mg·L^-1）	氢分压/MPa	pH	温度/℃
实验组	摇瓶（a）混合菌+4-BP+H₂	10	5	0.02	7.2	30
对照组	摇瓶（b）混合菌+4-BP+无H₂	10	5	0	7.2	30
	摇瓶（c）混合菌灭活+4-BP+无H₂	10	5	0	7.2	30
	摇瓶（d）混合菌灭活+4-BP+H₂	10	5	0.02	7.2	30

表3 摇瓶影响因素实验设计

Table 3 Experimental design of influencing factors of shaking bottle

项目	NO₃ ^--N/（mg·L^-1）	4-BP/（mg·L^-1）	氢分压/MPa	pH
4-BP	10	2	0.02	7.2
		5
		10
		20
NO₃ ^--N	5	20	0.02	7.2
	10
	20
	30
H₂	10	20	0.01	7.2
			0.02
			0.03
			0.04

图1 0.02 MPa下10 mg/L NO₃ ^--N的摇瓶运行情况

Fig. 1 Operation diagram of shake flask with 10 mg/L NO₃ ^--N at 0.02 MPa

图2 不同工况下摇瓶内4-BP的去除情况

Fig. 2 Removal of 4-BP in shake flasks under different working conditions

图3 4-BP浓度对4-BP还原降解的影响

Fig. 3 The effect of NO₃ ^--N concentration on the reduction and degradation of 4-BP

图4 NO₃ ^--N浓度对4-BP还原降解的影响

Fig. 4 The effect of NO₃ ^--N concentration on the reduction and degradation of 4-BP

图5 氢分压对4-BP还原降解的影响

Fig. 5 The effect of hydrogen partial pressure on the reduction and degradation of 4-BP

图6 微生物群落分析

Fig. 6 Microbial community analysis

表4 PICRUSt分析

Table 4 PICRUSt analysis

基因ID	KEGG通路描述	接种污泥	驯化后污泥
K00370	硝酸还原酶	3 921	10 998
K00371	硝酸还原酶	4 188	11 010
K01560	2-卤素脱卤素酶	5 128	11 328

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