摘要：

好氧颗粒污泥（AGS）技术在处理废水中有毒难降解有机物领域展现出一定的应用潜力，但在环烷酸（NAs）长期胁迫下AGS的形成过程和稳定性是否受到影响尚未可知。基于此，对比了环己烷甲酸（CHCA，20 mg/L）胁迫和常规培养条件下AGS形成特性、污染物去除性能和微生物群落结构的差异，结果表明，CHCA胁迫下可实现好氧污泥颗粒化，形成的AGS沉降性能良好，但平均粒径较小。CHCA的短期胁迫有利于EPS的分泌和颗粒的形成，但长期胁迫下造粒进程延缓，EPS分泌也受到抑制。CHCA存在下，AGS仍能维持良好的污染物去除性能，COD、NH₄ ⁺-N和TN平均去除率分别为92.6%、90.2%和70.5%，CHCA去除率达到99.5%。高通量测序结果表明，CHCA的存在会降低微生物群落多样性和丰富度，但会富集Azoarcus和unclassified_f_Aeromonadaceae，这类微生物有助于维持系统稳定和提升污染物去除效果。

关键词: 好氧颗粒污泥, 环烷酸, 序批式反应器, 微生物群落

Abstract:

Aerobic granular sludge(AGS) technology has shown certain application potential in the treatment of toxic and refractory organic matter in wastewater. However, it is unknown whether the formation process and stability of AGS are affected under long-term stress of naphthenic acids(NAs). Based on this, the formation characteristics, pollutant removal performance, and microbial community structure of AGS under cyclohexanecarboxylic acid (CHCA, 20 mg/L) stress and conventional culture conditions were compared. The results showed that aerobic sludge granulation could be achieved under CHCA stress, and the sedimentation performance of the formed AGS was good, with a smaller average particle size. The short-term stress of CHCA was beneficial for EPS secretion and granule formation, while the granulation process was delayed and EPS secretion was also inhibited under long-term stress. In the presence of CHCA, AGS could still maintain good pollutant removal performance, with average removal rates of 92.6%, 90.2%, and 70.5% for COD, NH₄ ⁺-N, and TN, respectively. The removal rate of CHCA reached 99.5%. The high-throughput sequencing results indicated that the presence of CHCA reduced microbial community diversity and richness, but enriched Azoarcus and unclassified_f_Aeromonadaceae, which helped to maintain system stability and improve pollutant removal effect.

Key words: aerobic granular sludge, naphthenic acid, sequencing batch reactors, microbial community

中图分类号: 

• X703