微丝菌培养分离、特性标记及调控研究进展

doi:10.11894/1005-829x.2017.37(10).001

工业水处理 ›› 2017, Vol. 37 ›› Issue (10): 1-5. doi: 10.11894/1005-829x.2017.37(10).001

• 专论与综述 • 下一篇

微丝菌培养分离、特性标记及调控研究进展

张振宇^1,2, 费学宁^1,2, 焦秀梅^1,2,3, 池勇志^1,2, 李松亚³

1. 天津城建大学, 天津 300384;
2. 天津市水质科学与技术重点实验室, 天津 300384;
3. 天津大学环境科学与工程学院, 天津 300072

收稿日期:2017-06-22 出版日期:2017-10-20 发布日期:2017-10-31
作者简介:张振宇(1991-),硕士.电话:13034388376,E-mail:852668799@qq.com.
基金资助:
国家自然科学基金面上项目（51578360）；天津市科委科技支撑重点项目（15ZCZDSF00150）。

Research progress in the characteristics,labelling and regulation of Microthrix parvicella

Zhang Zhenyu^1,2, Fei Xuening^1,2, Jiao Xiumei^1,2,3, Chi Yongzhi^1,2, Li Songya³

1. Tianjin Chengjian University, Tianjin 300384, China;
2. Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China;
3. School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China

Received:2017-06-22 Online:2017-10-20 Published:2017-10-31
Supported by:

摘要/Abstract

摘要： 污泥膨胀一直是困扰污水处理厂正常运行的一个世界性难题，而微丝菌是污泥丝状膨胀中的主要丝状菌，因此对微丝菌本质规律的研究不仅有利于认识微丝菌，也可提供诸多控制污泥丝状膨胀的方法。综述了微丝菌在培养分离、生理特性和影像识别以及调控等方面的研究进展，并对微丝菌的后续研究提出了建议和展望。

关键词: 污泥膨胀, 微丝菌, 微丝菌培养, 微丝菌识别, 微丝菌调控

Abstract: Sludge bulking has been a worldwide problem brothering the normal running of wastewater treatment plants. Microthrix parvicella is the main filamentous bacteria in sludge filamentous bulking. Therefore,the researches on the essential regulations of Microthrix parvicella not only help researchers to cognize Microthrix parvicella, but also provide a number of methods for the control of sludge bulking. The research progress in the cultivation, isolation,physiological characteristics,image identification and control measures of Microthrix parvicella are summarized,and suggestion and prospect on subsequent researches on Microthrix parvicella provided.

Key words: sludge bulking, Microthrix parvicella, Microthrix parvicella cultivation, Microthrix parvicella identification, Microthrix parvicella regulation

中图分类号:

X703

张振宇, 费学宁, 焦秀梅, 池勇志, 李松亚. 微丝菌培养分离、特性标记及调控研究进展[J]. 工业水处理, 2017, 37(10): 1-5.

Zhang Zhenyu, Fei Xuening, Jiao Xiumei, Chi Yongzhi, Li Songya. Research progress in the characteristics,labelling and regulation of Microthrix parvicella[J]. INDUSTRIAL WATER TREATMENT, 2017, 37(10): 1-5.

https://www.iwt.cn/CN/Y2017/V37/I10/1

参考文献

[1] Kristensen G H,Jorgensen P E,Nielsen P H. Settling characteristics of activated sludge in Danish treatment plants with biological nutrient removal[J]. Water Science & Technology,1994,29(7):157-165.
[2] Eikelboom D H,Andreadakis A,Andreasen K. Survey of filamentous populations in nutrient removal plants in four European countries[J]. Water Science & Technology,1998,37(4/5):281-289.
[3] Graveleau L,Cotteux E,Duchene P. Bulking and foaming in France: The 1999-2001 survey[J]. Clean-Soil,Air,Water,2010,33(3): 223-231.
[4] Madoni P,Davoli D,Gibin G. Survey of filamentous microorganisms from bulking and foaming activated-sludge plants in Italy[J]. Water Research,2000,34(6):1767-1772.
[5] Seviour E M,Williams C,Degrey B,et al. Studies on filamentous bacteria from australian activated sludge plants[J]. Water Research,1994,28(11):2335-2342.
[6] van Veen W L. Bacteriology of activated sludge,in particular the filamentous bacteria[J]. Antonie van Leeuwenhoek,1973,39(1): 189-205.
[7] Eikelboom D H. Filamentous organisms observed in activated sludge [J]. Water Research,1975,9(4):365-388.
[8] Slijkhuis H,Deinema M. Effect of environmental conditions on the occurrence of Microthrix parvicella in activated sludge[J]. Applied Environmental Microbiology,1988,46(4):832-839.
[9] Blackall L L,Seviour E M. Microthrix parvicella is a novel,deep branching member of the actionmycetes subphylum[J]. Systematic & Applied Microbiology,1995,17(4):513-518.
[10] Rossetti S,Christensson C,Blackall L L. Phenotypic and phylogenetic description of an Italian isolate of“Microthrix parvicella”[J]. Journal of Applied Microbiology,1997,82(4):405-410.
[11] Levantesi C,Rossetti S,Thelen K,et al. Phylogeny,physiology and distribution of‘Candidatus Microthrix calida’,a new Microthrix species isolated from industrial activated sludge wastewater treatment plants[J]. Environmental Microbiology,2006,8(9):1552-1563.
[12] Fei Xuening,Li Songya,Cao Lingyun,et al. A novel separation ethod of Microthrix parvicella filaments from activated sludge by a Hydrophobic Plate[J]. Current Microbiology,2015,71(4): 465-470.
[13] Slijkhuis H. Microthrix parvicella,a filamentous bacterium isolated from activated sludge:Cultivation in a chemically defined medium[J]. Applied & Environmental Microbiology,1983,46(4):832-839.
[14] Slijkhuis H,van Groenestijn J W,Kylstra D J. Microthrix parvicella,a filamentous bacterium from activated sludge:Growth on Tween 80 as carbon and energy source[J]. Journal of General Microbiology,1984,130(8):2035-2042.
[15] Tandoi V,Rossetti S,Blackall L L,et al. Some physiological properties of an Italian isolate of“Microthrix parvicella”[J]. Water Science & Technology,1998,37(4/5):1-8.
[16] Andreasen K,Nielsen P H. Growth of Microthrix parvicella in nutrient removal activated sludge plants:Studies of in situ physiology[J]. Water Research,2000,34(5):1559-1569.
[17] Nielsen P H,Roslev P,Dueholm T E,et al. Microthrix parvicella,a specialized lipid consumer in anaerobic-aerobic activated sludge plants[J]. Water Science & Technology,2002,46(1/2):73-80.
[18] Nielsen P H,Kragelund C,Seviour R J,et al. Identity and ecophysiology of filamentous bacteria in activated sludge[J]. Fems Microbiology Reviews,2009,33(6):969-998.
[19] Andreasen K,Nielsen P H. In situ characterization of substrate uptake by Microthrix parvicella using microautoradiography[J]. Water Science & Technology,1998,37:19-26.
[20] Wang Juan,Qi Rong,Liu Miaomiao,et al. The potential role of ‘Candidatus Microthrix parvicella’in phosphorus removal during sludge bulking in two full-scale enhanced biological phosphorus removal plants[J]. Water Science & Technology,2014,70(2):367-375.
[21] Mcillroy S J,Kristiansen R,Albertsen M,et al. Metabolic model for the filamentous‘Candidatus Microthrix parvicella’based on genomic and metagenomic analyses[J]. Isme Journal,2013,7(6): 1161-1172.
[22] Rossetti S,Tomei M C,Nielsen P H,et al.“Microthrix parvicella”, a filamentous bacterium causing bulking and foaming in activated sludge systems:A review of current knowledge[J]. Fems Microbiology Reviews,2005,29(1):49-64.
[23] Erhart R,Bradford D,Seviour R J,et al. Development and use of fluorescent in situ hybridization probes for the detection and identification of Microthrix parvicella in activated sludge[J]. Systematic & Applied Microbiology,1997,20(2):310-318.
[24] 叶倩. PCR技术综述[J]. 科技创新导报,2009(5):5-6.
[25] 王润芳,张红,王琴,等. 微丝菌(Microthrix parvicella)原位荧光杂交(FISH)定量过程的条件优化[J]. 环境科学,2016,37(6): 2266-2270.
[26] Vanysacker L,Denis C,Roels J,et al. Development and evaluation of a TaqMan duplex real-time PCR quantification method for reliable enumeration of Candidatus Microthrix[J]. Journal of Microbiological Methods,2014,97(1):6-14.
[27] Dunkel T,Dias P A,Gallegos E L D L,et al. In situ microscopy as a tool for the monitoring of filamentous bacteria:A case study in an industrial activated sludge system dominated by M. parvicella[J]. Water Science & Technology,2015,73(6):1333-1340.
[28] Li Songya,Fei Xuening,Jiao Xiumei,et al. Synthesis and characterization of the fluorescent probes for the labeling of Microthrix parvicella[J]. Applied Microbiology & Biotechnology,2016,100 (6):2883-2894.
[29] Fei Xuening,Sun Wenke,Cao Lingyun. Design and preparation of quantum dots fluorescent probes for in situ identification of Microthrix parvicella in bulking sludge[J]. Applied Microbiology & Biotechnology,2016,100(2):961-968.
[30] 郝亚超. 微丝菌特异性识别荧光探针设计制备及识别特性[D]. 天津:天津大学,2014.
[31] Wang Juan,Li Qian,Qi Rong,et al. Sludge bulking impact on relevant bacterial populations in a full scale municipal wastewater treatment plant[J]. Process Biochemistry,2014,49(12):2258-2265.
[32] Wang Ping,Yu Zhisheng,Zhao Jihong,et al. Seasonal changes in bacterial communities cause foaming in a wastewater treatment plant[J]. Microbial Ecology,2016,71(3):660-677.
[33] Pradhan S K. Iron flocculation stimulates biogas production in Microthrix parvicella-spiked wastewater sludge[J]. International Journal of Environmental Science and Technology,2015,12(9):3039-3046.
[34] Leven L,Wijnbladh E,Tuvesson M,et al. Control of Microthrix parvicella and sludge bulking by ozone in a full-scale WWTP[J]. Water Science & Technology,2016,73(4):866-872.
[35] Lienen T,Kleybcker C A,Verstraete W,et al. Moderate temperature increase leads to disintegration of floating sludge and lower abundance of the filamentous bacterium Microthrix parvicella in anaerobic digesters[J]. Water Research,2014,65:203-212.

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