Abstract:

In order to investigate the effects of temperature changes on the morphological structure, pollutant removal capacity and microbial community structure of granular sludge acclimated by different carbon sources, the enhanced biological phosphorus removal(EBPR) system of sodium acetate and sodium propionate at 10℃, 20℃ and 25℃ was studied in an SBR reactor. The results showed that the temperature rise would lead to the sludge disintegration in the two kinds of granular sludge homogenization systems, and the sludge sedimentation performance deteriorated. The average particle size dropped from 650 μm to 200 μm. The removal rate of PO₄^3- decreased from more than 99% to 56% and 88% respectively, and the COD removal rate changed little. Microbial abundance and diversity were the highest at 20℃ and the lowest at 25℃, among of which Proteobacteria, Bacteroidetes, Verrucomicrobia and Planctomycetes accounted for more than 95% of all the bacteria in the two systems in total during the temperature change. The deterioration of phosphorus removal in the sodium acetate system is due to the growth of glycogen accumulating organisms, while the deterioration of the sodium propionate system may be related to the migration of phosphorus accumulating organisms.

Key words: enhanced biological phosphorus removal, aerobic granular sludge, temperature, microbial community structure

摘要：

为了考察温度变化对不同碳源驯化的颗粒污泥形态结构、污染物去除能力以及微生物群落结构的影响，采用SBR反应器，对10、20、25℃条件下的乙酸钠和丙酸钠强化生物除磷（EBPR）颗粒污泥系统进行了研究。结果表明，温度升高使得2种颗粒污泥系统均发生了污泥解体现象，污泥沉降性能恶化，平均粒径均由650 μm降至200 μm；PO₄^3-去除率由99%以上分别下降至56%和88%，COD去除率变化较小；微生物丰度与多样性在20℃时最高，25℃时最低，其中变形菌门、拟杆菌门、疣微菌门、浮霉菌门这4类在温度变化中总共均能占两系统中所有菌门的95%以上；乙酸钠系统除磷恶化是由于聚糖菌的增长，而丙酸钠系统的恶化可能与聚磷菌自身代谢的迁移有关。

关键词: 强化生物除磷, 好氧颗粒污泥, 温度, 菌群结构

CLC Number: 

• X703.1