Cultivation and treatment characteristics of Chlorella-aerobic sludge coupled system

doi:10.19965/j.cnki.iwt.2023-0888

Abstract

Abstract:

Aerobic granular sludge was cultivated as a biological carrier under laboratory conditions, and Chlorella was inoculated to form algal-bacterial granular sludge(ABGS). The effects of different light intensities on pollutant removal efficiency of ABGS system were explored, and the enhancement of Chlorella on the treatment capacity of ABGS system under 5 klx light intensity was emphatically investigated. The results showed that the removal rates of COD, TN and TP by ABGS were 87.01%-95.32%, 53.14%-89.37% and 32.49%-99.40%, respectively, with the SND of 7.70%-83.66%. However, the removal rates of TP and TN in a single Chlorella system were only 20%-30%, while the removal effect of COD was not obvious. Chlorella could increase the concentration of dissolved oxygen in water within a certain light intensity range, and facilitate the degradation of pollutants by promoting the metabolic rate of bacteria in ABGS system. With the increase of light intensity, the removal rates of COD and TP in ABGS system increased, while the removal rates of TN and SND decreased. Too low or too high light intensity would inhibit the photosynthesis and even kill Chlorella, leading to the disintegration of ABGS, system collapse and poor pollutant removal effect. High throughput analysis showed that the addition of algae further expanded the relative abundance of bacteria related to nitrogen and phosphorus metabolism, and enhanced the nitrogen and phosphorus removal effect of the system.

Key words: ABGS system, aerobic granular sludge, bacteria and algae symbiosis, nitrogen and phosphorus removal

摘要：

在实验室条件下培养出好氧颗粒污泥作为生物载体，接种小球藻形成菌藻共生颗粒污泥（Algal-bacterial granular sludge，ABGS），探究不同光照强度对ABGS系统去除污染物效果的影响，重点考察了5 klx光照强度下小球藻对ABGS系统处理能力的增强作用。结果表明，在0~100 klx光照强度范围内，ABGS对COD、TN和TP的去除率分别为87.01%~95.32%、53.14%~89.37%和32.49%~99.40%，同步硝化反硝化率（SND）为7.70%~83.66%；而单一小球藻系统对TP和TN的去除率仅为20%~30%，对COD去除效果不明显。在一定光照强度范围内，小球藻可以提高水中DO，并通过促进ABGS系统中细菌的代谢速率来促进污染物的降解。随着光照强度的增强，ABGS系统对COD和TP的去除率上升，对TN的去除率下降，SND下降。过低或过高的光照强度均会抑制小球藻的光合作用甚至使其死亡，导致ABGS破裂解体，系统崩溃，污染物去除效果变差。高通量分析表明，藻类的加入进一步扩大了与氮磷代谢相关的菌种相对丰度，增强了系统的脱氮除磷效果。

关键词: ABGS系统, 好氧颗粒污泥, 菌藻共生, 脱氮除磷

CLC Number:

X703

Linli GAO, Chengda HE, Qi CHENG. Cultivation and treatment characteristics of Chlorella-aerobic sludge coupled system[J]. Industrial Water Treatment, 2024, 44(9): 160-168.

高林利, 何成达, 程琪. 小球藻-好氧污泥耦合颗粒的培养及处理特性研究[J]. 工业水处理, 2024, 44(9): 160-168.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2023-0888

https://www.iwt.cn/EN/Y2024/V44/I9/160

Figures/Tables 13

Fig.1 Schematic diagram of experimental device

Table 1 Operating time of each stage of the device with culture time

1~3

4~10

10~120

175

125

485

295~305

230~248

30→20

20→2

Fig.2 Morphological changes during sludge culture

Fig.3 Removal effect of COD by ABGS under different light intensities

Fig.4 Removal effect of TN by ABGS under different light intensities

Fig.5 Removal effect of NH₄ ⁺-N，NO₃ ^--N and NO₂ ^--N（a），and removal rate of NH₄ ⁺-N（b） by ABGSunder different light intensities

Fig.6 Removal effect of TP by ABGS under different light intensities

Fig.7 Granular sludge morphology under 5 klx（a） and 0 lx（b） light intensities

Fig.8 Pollutants removal effect under 5 klx and 0 lx light intensities

Fig.9 Removal effect of Chlorella on pollutants

Fig.10 SEM of AGS and ABGS

Table 2 Comparison of microbial diversity index of samples

664

601

739.788

722.852

750.254

711.590

0.071

0.163

4.129

3.152

0.996

Fig.11 Distribution of biological functional communities at phylum level（a） and genus level（b）

References 27

1	ZHANG Han， GONG Weijia， ZENG Weichen，et al. Organic carbon promotes algae proliferation in membrane-aeration based bacteria-algae symbiosis system（MA-BA）［J］. Water Research，2020，176：115736. doi:10.1016/j.watres.2020.115736
2	ZHANG Bing， LI Wei， GUO Yuan，et al. Microalgal-bacterial consortia：From interspecies interactions to biotechnological applications［J］. Renewable and Sustainable Energy Reviews，2020，118：109563. doi:10.1016/j.rser.2019.109563
3	DONG Xiaochuan， ZHAO Ziwen， YANG Xiaojing，et al. Response and recovery of mature algal-bacterial aerobic granular sludge to sudden salinity disturbance in influent wastewater：Granule characteristics and nutrients removal/accumulation［J］. Bioresource Technology，2021，321：124492. doi:10.1016/j.biortech.2020.124492
4	XIAO Guixing， CHEN Jianqiu， SHOW P L，et al. Evaluating the application of antibiotic treatment using algae-algae/activated sludge system［J］. Chemosphere，2021，282：130966. doi:10.1016/j.chemosphere.2021.130966
5	李竺芯，樊迪，那娜，等. 光照条件对菌藻共生系统单级脱氮的影响研究［J］. 环境科学与技术，2019，42（10）：78-82. doi:10.19672/j.cnki.1003-6504.2019.10.012
	LI Zhuxin， FAN Di， NA Na，et al. Study on the effect of light condition on single-stage nitrogen removal in bacteria-algae symbiosis system［J］. Environmental Science & Technology，2019，42（10）：78-82. doi:10.19672/j.cnki.1003-6504.2019.10.012
6	程琪. 自耦合微藻-细菌颗粒污泥特性研究［D］. 扬州：扬州大学，2022.
	CHENG Qi. Study on characteristics of self-coupling microalgae-bacterial granular sludge［D］. Yangzhou：Yangzhou University，2022.
7	NANCHARAIAH Y V， KIRAN KUMAR REDDY G. Aerobic granular sludge technology：Mechanisms of granulation and biotechnological applications［J］. Bioresource Technology，2018，247：1128-1143. doi:10.1016/j.biortech.2017.09.131
8	IORHEMEN O T， LIU Yang. Effect of feeding strategy and organic loading rate on the formation and stability of aerobic granular sludge［J］. Journal of Water Process Engineering，2021，39：101709. doi:10.1016/j.jwpe.2020.101709
9	刘琳，叶嘉琦，刘玉洪，等. 好氧污泥-微藻耦合颗粒的培养及特性研究［J］. 中国环境科学，2017，37（7）：2536-2541.
	LIU Lin， YE Jiaqi， LIU Yuhong，et al. Research on the development and properties of aerobic sludge-microalgae granular system［J］. China Environmental Science，2017，37（7）：2536-2541.
10	FAN Xiaoyan， GAO Jingfeng， PAN Kailing，et al. Shifts in bacterial community composition and abundance of nitrifiers during aerobic granulation in two nitrifying sequencing batch reactors［J］. Bioresource Technology，2018，251：99-107. doi:10.1016/j.biortech.2017.12.038
11	李伟健，苏雷，赵子萱，等. 好氧颗粒污泥处理高氨氮废水的研究进展［J］. 辽宁化工，2022，51（9）：1273-1275.
	LI Weijian， SU Lei， ZHAO Zixuan，et al. Research progress on treatment of high ammonia nitrogen wastewater by aerobic granular sludge［J］. Liaoning Chemical Industry，2022，51（9）：1273-1275.
12	唐瑾，徐志昂，黄斌，等. 光强对小球藻生长及有机物含量的影响研究［J］. 太阳能学报，2020，41（8）：370-374.
	TANG Jin， XU Zhiang， HUANG Bin，et al. Effect of light intensity on growth and algal organic matter content of Chlorella sp. cultivation［J］. Acta Energiae Solaris Sinica，2020，41（8）：370-374.
13	CAI Ting， PARK S Y， LI Yebo. Nutrient recovery from wastewater streams by microalgae：Status and prospects［J］. Renewable and Sustainable Energy Reviews，2013，19：360-369. doi:10.1016/j.rser.2012.11.030
14	HE Qingming， PENG Xuya， LI Zhiyang. The treatment of animal manure wastewater by coupled simultaneous methanogenesis and denitrification（SMD） and shortcut nitrification-denitrification（SND）［J］. Journal of Chemical Technology & Biotechnology，2014，89（11）：1697-704. doi:10.1002/jctb.4244
15	彭赵旭，霍明昕，彭永臻，等. DO对除磷过程的长期影响［J］. 哈尔滨工业大学学报，2011，43（4）：42-46. doi:10.11918/j.issn.0367-6234.2011.04.009
	PENG Zhaoxu， HUO Mingxin， PENG Yongzhen，et al. Long-term effect of DO on phosphorus removal process［J］. Journal of Harbin Institute of Technology，2011，43（4）：42-46. doi:10.11918/j.issn.0367-6234.2011.04.009
16	WENG Dongchen， PENG Yongzhen， WANG Xiaoxia，et al. Inhibition of nitrite on denitrifying phosphate removal process［J］. Advanced Materials Research，2014，955/956/957/958/959：1944-1950.
17	ZENG Wei， YANG Yingying， LI Lei，et al. Effect of nitrite from nitritation on biological phosphorus removal in a sequencing batch reactor treating domestic wastewater［J］. Bioresource Technology，2011，102（12）：6657-6664. doi:10.1016/j.biortech.2011.03.091
18	OEHMEN A， LEMOS P C， CARVALHO G，et al. Advances in enhanced biological phosphorus removal：From micro to macro scale［J］. Water Research，2007，41（11）：2271-2300. doi:10.1016/j.watres.2007.02.030
19	GRIFFITHS P C， STRATTON H M， SEVIOUR R J. Environmental factors contributing to the“G bacteria” population in full-scale EBPR plants［J］. Water Science and Technology，2002，46（4/5）：185-192. doi:10.2166/wst.2002.0583
20	KUMAR A， BERA S. Revisiting nitrogen utilization in algae：A review on the process of regulation and assimilation［J］. Bioresource Technology Reports，2020，12：100584. doi:10.1016/j.biteb.2020.100584
21	LAU P S， TAM N F Y， WONG Y S. Effect of algal density on nutrient removal from primary settled wastewater［J］. Environmental Pollution，1995，89（1）：59-66. doi:10.1016/0269-7491(94)00044-e
22	李冬，柴晨旭，李帅，等. 运行模式对菌藻共生颗粒污泥系统的影响［J］. 中国环境科学，2023，43（9）：4718-4727.
	LI Dong， CHAI Chenxu， LI Shuai，et al. Influence of operation mode on the bacterial-algal symbiotic granular sludge system［J］. China Environmental Science，2023，43（9）：4718-4727.
23	TANG Peng， YU Deshuang， CHEN Guanghui，et al. Novel aerobic granular sludge culture strategy：Using granular sludge Anammox process effluent as a biocatalyst［J］. Bioresource Technology，2019，294：122156. doi:10.1016/j.biortech.2019.122156
24	李伟. 菌藻共生好氧颗粒污泥的形成机理及其资源化利用研究［D］. 哈尔滨：哈尔滨工业大学，2020.
	LI Wei. Study on the formation mechanism and resource utilization of aerobic granular sludge with symbiotic bacteria and algae［D］. Harbin：Harbin Institute of Technology，2020.
25	陈庆峰，余哲，黄诗琪，等. 菌藻共生好氧颗粒污泥的分形特征研究［J］. 广西师范大学学报（自然科学版），2022，40（6）：163-172.
	CHEN Qingfeng， YU Zhe， HUANG Shiqi，et al. Study on fractal characteristics of aerobic granular sludge with symbiotic bacteria and algae［J］. Journal of Guangxi Normal University（Natural Science Edition），2022，40（6）：163-172.
26	MÉVEL G， PRIEUR D. Heterotrophic nitrification by a thermophilic Bacillus species as influenced by different culture conditions［J］. Canadian Journal of Microbiology，2000，46（5）：465-473. doi:10.1139/w00-005
27	相延铮，何成达，朱腾义，等. 碳源对除磷颗粒污泥去除效果和微生物特性的影响［J］. 工业水处理，2023，43（12）：89-95.
	XIANG Yanzheng， HE Chengda， ZHU Tengyi，et al. Effect of carbon source on removal efficiency and microbial characteristics of phosphorus removal granular sludge［J］. Industrial Water Treatment，2023，43（12）：89-95.

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