Abstract:

The effective control of endogenous contamination of black and odorous water has become an important work in China. Phytoremediation technology is more widely used, and microbial agent method is gradually accepted due to the absence of chemical agents. In this study, microbial enhanced phytoremediation technology was used to purify black and odorous water, the removal effects of single microorganisms, single plants, and microorganisms-plant combinations on endogenous pollutants such as nitrogen and phosphorus in sediment were investigated. The results showed that the combination of microorganisms and plants exhibited better removal effect of total nitrogen and total phosphorus. Moreover, after 56 days, the removal rates of total nitrogen and total phosphorus in the sediment were 43.59% and 52.10%, respectively, which increased by 8.74% and 5.27% compared with that of single microorganisms system, and increased by 19.47% and 9.76% compared with that of single plants system. The synergistic effect of microorganisms and plants could increase the release and absorption rates of nitrogen and phosphorus in sediment, while promote nitrogen and phosphorus cycling. The high-throughput sequencing results showed that the dominant phylum in the microorganism-plant system was Proteobacteria (relative abundance 54%). The dominant phylum was Gammaproteobacteria (49%), and the dominant genus was Cavicella (10%). Microbial enhanced phytoremediation technology could provide technical support for the treatment of black and odorous sediment pollution.

Key words: black-odor water, endogenous pollution, microbial-plant purification, microbial community structure

摘要：

黑臭水体底泥内源污染的有效治理已成为我国生态文明建设的重要工作。植物修复技术应用较广泛，微生物菌剂因不外加化学药剂而逐渐被认可。采用微生物强化植物修复技术净化黑臭水体，考察单一微生物、单一植物、微生物-植物联合对底泥中氮、磷等内源污染物的去除效果。结果表明：脱氮解磷菌和植物联合技术表现出对TN和TP等污染物较好的去除效果，反应器运行56 d后，底泥中TN和TP的去除率分别为43.59%和52.10%，较单一微生物组分别提升8.74%和5.27%，较单一植物组分别提升19.47%和9.76%；微生物与植物协同作用，可增大底泥中氮、磷的释放和吸收速率，促进氮、磷循环。微生物高通量测序结果表明，微生物-植物体系中优势菌门为变形菌门（Proteobacteria，相对丰度54%），优势菌纲为γ-变形菌纲（Gammaproteobacteria，相对丰度49%），优势菌属为空洞菌属（Cavicella，相对丰度10%）。微生物强化植物修复技术可为黑臭水体底泥内源污染的治理提供技术支撑。

关键词: 黑臭水体, 内源污染, 微生物-植物净化, 微生物群落结构

CLC Number: 

• X703

• X52