Effect of salinity on performance and microbial activity of sequential batch biofilm reactor

Abstract

Abstract:

In order to explore the influence of salinity change on sequential batch biofilm reactor（SBBR） wastewater treatment，three kinds of fillers（white breathing ring，biological rope type packing and polyurethane sponge suspension balls） were used for membrane hanging，and two salt-tolerant strains，Mangrovibacter yixingensis and Thauera selenatis，were added. The degradation effects of COD，NH₄ ⁺-N，NO₃ ^--N and NO₂ ^--N and their effects on the main characteristics of biofilms（SOUR，SAOR，SNOR，SNRR and EPS） were investigated at the salinity of 0.5%，1%，2%，3%，4% and 5%，respectively. The results showed that the COD degradation rate and NH₄ ⁺-N removal rate of SBBR were about 90% and 80% respectively in the range of 0.5%-3% salinity. Among nitrite oxidizing bacteria，ammonia oxidizing bacteria and nitrate reducing bacteria，salinity had the greatest inhibitory effect on nitrite oxidizing bacteria，then followed by ammonia oxidizing bacteria，and the least inhibitory effect on nitrate reducing bacteria. PN and PS in LB-EPS and TB-EPS in the three reactors increased with increasing salinity，while PN/PS ratio decreased with increasing salinity，and PS was more significantly affected by salinity. Compared with biological rope type packing and polyurethane sponge suspension ball，white breathing ring had stronger impact resistance，less affected by salinity changes，and more abundant microbial population. With the increase of salinity，the dominant microbial populations of the three fillers were significantly different. After the addition and domestication of salt-tolerant bacteria，Mangrovibacter became the dominant bacterial genus.

Key words: SBBR, COD degradation, denitrification rate, microbial enzyme activity, microbial community structure

摘要：

为探究盐度对序批式生物膜反应器（SBBR）处理废水的影响，采用3种填料（白呼吸环、生物绳型填料、聚氨酯海绵悬浮球）对SBBR进行挂膜，且投加已筛选出的2种耐盐菌：宜兴曼格洛杆菌（Mangrovibacter yixingensis）和索氏菌属砷酸索氏菌（Thauera selenatis），分别在盐度0.5%、1%、2%、3%、4%、5%条件下考察其对COD、NH₄ ⁺-N、NO₃ ^--N和NO₂ ^--N的降解效果以及对生物膜主要特性（SOUR、SAOR、SNOR、SNRR和EPS）的影响。结果表明，在盐度0.5%~3%范围内SBBR对COD降解率约90%，NH₄ ⁺-N去除率约80%；盐度对菌群的抑制作用为亚硝酸盐氧化菌>氨氧化菌>硝酸盐还原菌；3种反应器中LB-EPS、TB-EPS中的PN和PS都随盐度的增加而增加，PN/PS随盐度的增加而降低，PS受盐度的影响更为明显；白呼吸环比生物绳型填料和聚氨酯海绵悬浮球具有更强的抗冲击能力，受盐度变化的影响较小，微生物种群更丰富；随着盐度的提高，3种填料的微生物优势种群有明显差异，经过耐盐菌的投加与驯化，Mangrovibacter成为优势菌属。

关键词: SBBR, COD降解, 脱氮速率, 微生物酶活性, 微生物群落结构

CLC Number:

X703.1

Hongxue AN, Wanyu ZHANG, Fei XUE, Yuwei BAI, Jing NING, Zaixing LI. Effect of salinity on performance and microbial activity of sequential batch biofilm reactor[J]. Industrial Water Treatment, 2023, 43(6): 117-124.

安鸿雪, 张婉玉, 薛飞, 白玉玮, 宁静, 李再兴. 盐度对序批式生物膜反应器性能及微生物活性影响[J]. 工业水处理, 2023, 43(6): 117-124.

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URL: https://www.iwt.cn/EN/Y2023/V43/I6/117

Figures/Tables 10

Fig. 1 SBBR device

Table 1 Changes in the amount of biofilm in the reactors

阶段	SBBR1生物膜量/（mg·mm^-2）	SBBR2生物膜量/（mg·mm^-2）	SBBR3生物膜量/（mg·mm^-2）
挂膜前期	0.119	0.154	0.131
挂膜中期	0.263	0.289	0.271
挂膜后期	0.467	0.433	0.451

Fig. 2 Comparison before and after packing film

Fig. 3 COD removal effect under different salinities

Fig. 4 Removal effects of NH₄ ⁺-N（a），NO₃ ^--N（b） and NO₂ ^--N（c） at different salinities

Fig. 5 Effect of salinity change on SOUR of biofilm

Fig. 6 Effect of salinity changes on SAOR （a），SNOR （b） and SNRR （c） of biofilms

Fig. 7 Effects of salinity changes on biofilm LB-EPS （a），TB-EPS （b） and PN/PS （c）

Fig. 8 Relative abundance of bacteria at phylum distribution level

Fig. 9 Relative abundance of bacteria at genus distribution level

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