Abstract:

The landfill，pharmaceutical，and mining industries generate large amount of wastewater containing sulfides（S^2-） and nitrogen elements，which may cause water pollution and endanger the survival of aquatic animals and plants. Total nitrogen and S^2- were simultaneously removed from water by sulfide autotrophic denitrification. Through batch experiments，the Box-Behnken Design response surface methodology was used to screen the optimal reaction conditions for autotrophic denitrification with sulfide as electron donor. The results showed that under the condition of pH 7.05，temperature 31.98 ℃ and n _N/n _S 0.75，the TN removal rate was the best and the average removal rate was 95.53%. A dynamic flow pilot reactor was constructed to investigate its advanced nitrogen removal performance based on the batch results. During 30 days of operation，when the HRT was 5 h， the maximum removal rate of TN was 94.31%. The scanning electron microscope showed that a large number of rod-shaped bacterium were attached to the surface of filler. The sludges from the upper，middle and lower parts of the reactor were taken for microbial high-throughput analysis，and the results showed that all the dominant bacteria were Thiobacillus，a typical sulfur-autotrophic denitrification functional bacterium，with abundances of 46.39%，46.31% and 49.99%， respectively.

Key words: sulfur autotrophic denitrification, sulfide, response surface methodology, microbial community structure

摘要：

垃圾填埋、制药和采矿行业会产生大量含有硫化物（S^2-）和氮元素的废水，污染水体，危害水中动植物的生存。采用硫化物自养反硝化技术同时脱除水体中的总氮和S^2-，通过静态批次实验和Box-Behnken Design响应曲面法对以S^2-为电子供体的自养反硝化反应条件进行筛选。结果表明：pH为7.05、温度为31.98 ℃和n _N/n _S为0.75时，对TN去除效果最好，平均去除率为95.53%。在此基础上，构建动态流小试反应器，设置HRT为5 h，考察其深度脱氮性能，在为期30 d的运行中，TN最大去除率为94.31%。通过扫描电镜发现填料表面附着大量杆状细菌，对反应器上中下部污泥分别进行微生物高通量分析，优势菌属均为典型的硫自养反硝化功能菌Thiobacillus（硫杆菌属），丰度分别为46.39%、46.31%和49.99%。

关键词: 硫自养反硝化, 硫化物, 响应曲面法, 菌群结构

CLC Number: 

• X703.1