A study on the antibiotic resistance genes in different tetracycline wastewater treatment systems

doi:10.11894/iwt.2021-0254

Abstract

Abstract:

Two types of reactors, sequencing batch reactor(SBR) and moving bed biofilm reactor(MBBR), were operated to treat wastewater with different concentrations of tetracycline. It was found that the removal efficiencies of total nitrogen and tetracycline in the biofilm system were better than that in the activated sludge system when the biomass concentrations were at the same level. Metagenomic analysisbase based on high-throughput sequencing showed that the different antibiotic resistance genes(ARGs) diversities and abundances in activated sludge and biofilm might be the primary causes of the difference in pollutant removal efficiency. Further analysis of the microbial community and their correlations with ARGs suggested that the microbial community structures were highly associated with ARGs. The different diversities and abundances of ARGs in SBR and MBBR were mainly caused by the different microbial community, which also affected the pollustant removal efficiency of the two systems.

Key words: activated sludge, biofilm, antibiotic resistance genes(ARGs), microbial community structure

摘要：

通过序批式反应器(SBR)和移动床生物膜反应器(MBBR)两种类型反应器的运行比较了活性污泥和生物膜工艺对不同浓度四环素废水中污染物的去除效果，发现在相同生物量条件下生物膜系统对总氮和四环素的去除效果优于活性污泥系统。基于高通量测序的宏基因组学分析结果表明，活性污泥和生物膜中抗生素抗性基因(ARGs)种类和丰度的差异可能是导致污染物去除效果不同的主要原因。进一步对活性污泥和生物膜中微生物群落结构及其与ARGs的相关性进行分析，证实了微生物群落结构与ARGs组成显著相关，是造成活性污泥和生物膜中ARGs差异的主要原因，进而影响了两种工艺对四环素废水中污染物的去除。

关键词: 活性污泥, 生物膜, 抗生素抗性基因(ARGs), 微生物群落结构

CLC Number:

X703

Haohao Sun,Kailong Huang,Xuxiang Zhang,Hongqiang Ren,Lin Ye. A study on the antibiotic resistance genes in different tetracycline wastewater treatment systems[J]. Industrial Water Treatment, 2021, 41(6): 105-110.

孙浩浩,黄开龙,张徐祥,任洪强,叶林. 不同四环素废水处理系统中的抗生素抗性基因研究[J]. 工业水处理, 2021, 41(6): 105-110.

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URL: https://www.iwt.cn/EN/10.11894/iwt.2021-0254

https://www.iwt.cn/EN/Y2021/V41/I6/105

Figures/Tables 5

References 22

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反应器	阶段	四环素/(μg·L^-1)	COD/(mg·L^-1)	NH4+-N/(mg·L^-1)	TP/(mg·L^-1)	HRT/h
Control-SBR		0	300	60	5	8
Control-MBBR		0
SBR	Phase Ⅰ 1~30 d	50
	Phase Ⅱ 31~60 d	500
	Phase Ⅲ 61~90 d	1 000
MBBR	Phase Ⅰ 1~30 d	50
	Phase Ⅱ 31~60 d	500
	Phase Ⅲ 61~90 d	1 000

反应器	阶段	四环素/(μg·L^-1)	COD/(mg·L^-1)	NH4+-N/(mg·L^-1)	TP/(mg·L^-1)	HRT/h
Control-SBR		0	300	60	5	8
Control-MBBR		0
SBR	Phase Ⅰ 1~30 d	50
	Phase Ⅱ 31~60 d	500
	Phase Ⅲ 61~90 d	1 000
MBBR	Phase Ⅰ 1~30 d	50
	Phase Ⅱ 31~60 d	500
	Phase Ⅲ 61~90 d	1 000

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