Screening, compounding and evaluation of decolorization efficiency of azo dye decolorizing bacteria

doi:10.11894/iwt.2021-0313

Abstract

Abstract:

Four highly-efficient decolorizing bacteria screened from the domesticated activated sludge of the landfill leachate treatment system were identified as Aneurinibacillus sp. YR-2, Stenotrophomonas acidaminiphila YR-3, Citrobacter freundii HR-1 and Klebsiella sp. HR-3, respectively. The four strains were compounded according to the same volume to obtain the optimum bacterial consortium, and the universality of the bacterial consortium was further investigated. The results showed that the complex microbial flora composed of YR-2, YR-3 and HR-3 had the fastest decolorization rate and the highest efficiency, which was determined as the optimum functional bacterial consortium. The bacterial consortium was alkali-resistant and halotolerant. The tolerance range to pH and salinity were 7-10 and 0%-10%, respectively. Furthermore, the decolorization rates of the bacterial consortium for azo dyes, heterocyclic dyes and triphenylmethane dye were all above 92%, indicating the functional bacterial consortium had a good decolorization broadspectrum.

Key words: functional bacterial consortium, isolation and identification, decolorization, dye wastewater

摘要：

通过定向驯化从垃圾渗滤液处理系统活性污泥中筛选获得4株高效脱色菌，经鉴定分别为A neurinibacillus sp.YR-2、Stenotrophomonas acidaminiphila YR-3、Citrobacter freundii HR-1及Klebsiella sp.HR-3。按等体积将4株菌的菌液进行复配，获得最佳复配菌群，并进一步考察其普适性。结果显示，由YR-2、YR-3、HR-3组成的复配菌群脱色速率最快、效率最高，为最佳复配菌群。该菌群耐碱耐高盐，适应pH范围为7~10，耐受盐度范围为0%~10%。此外，该菌群对偶氮类染料、杂环类染料及三苯甲烷染料脱色率均高达92%以上，具有良好的脱色广谱性。

关键词: 功能菌群, 分离鉴定, 脱色, 染料废水

CLC Number:

X703

Qi Luo,Qing Yang,Kailong Huang,Lin Ye,Jie Lu,Xuxiang Zhang,Hongqiang Ren. Screening, compounding and evaluation of decolorization efficiency of azo dye decolorizing bacteria[J]. Industrial Water Treatment, 2021, 41(6): 111-118.

罗琦,杨庆,黄开龙,叶林,陆杰,张徐祥,任洪强. 偶氮染料脱色菌的筛选、复配及脱色效能评估[J]. 工业水处理, 2021, 41(6): 111-118.

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URL: https://www.iwt.cn/EN/10.11894/iwt.2021-0313

https://www.iwt.cn/EN/Y2021/V41/I6/111

Figures/Tables 8

References 43

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染料名称	分类	最大吸收峰/nm	相对分子质量	分子结构
中性红	碱性醌亚胺染料	479.5	288.78
甲基橙	酸性偶氮染料	464	327.33
刚果红	直接偶氮染料	486	696.68
亚甲基蓝	碱性吩噻嗪染料	664	373.12
孔雀石绿	阴离子三苯甲烷染料	617	364.92
活性艳红X-3B	活性偶氮染料	538	615.33
活性黑5	活性偶氮染料	598	991.82

染料名称	分类	最大吸收峰/nm	相对分子质量	分子结构
中性红	碱性醌亚胺染料	479.5	288.78
甲基橙	酸性偶氮染料	464	327.33
刚果红	直接偶氮染料	486	696.68
亚甲基蓝	碱性吩噻嗪染料	664	373.12
孔雀石绿	阴离子三苯甲烷染料	617	364.92
活性艳红X-3B	活性偶氮染料	538	615.33
活性黑5	活性偶氮染料	598	991.82

菌株	菌落特征	革兰氏染色	细胞特征
YR-2	乳白色、不透明、边缘不整齐、表面干燥	阳性	杆菌
YR-3	黄白色、不透明，边缘整齐、表面湿润	阴性	杆菌
HR-1	灰白色、不透明、边缘不整齐，表面有黏性	阴性	杆菌
HR-3	灰白色、不透明、边缘整齐，表面有黏性	阴性	杆菌

菌株	菌落特征	革兰氏染色	细胞特征
YR-2	乳白色、不透明、边缘不整齐、表面干燥	阳性	杆菌
YR-3	黄白色、不透明，边缘整齐、表面湿润	阴性	杆菌
HR-1	灰白色、不透明、边缘不整齐，表面有黏性	阴性	杆菌
HR-3	灰白色、不透明、边缘整齐，表面有黏性	阴性	杆菌

菌株组合	脱色率/%
菌株组合	24 h	48 h
YR-2∶YR-3(1∶1)	64.10	93.45
YR-2∶HR-1(1∶1)	48.59	62.94
YR-2∶HR-3(1∶1)	54.23	95.27
YR-3∶HR-1(1∶1)	45.62	66.82
YR-3∶HR-3(1∶1)	63.82	96.95
HR-1∶HR-3(1∶1)	58.65	97.68
YR-2∶YR-3∶HR-3(1∶1∶1)	67.08	97.18
YR-2∶YR-3∶HR-1(1∶1∶1)	49.18	87.23
YR-2∶HR-1∶HR-3(1∶1∶1)	54.56	92.21
YR-3：HR-1：HR-3(1∶1∶1)	59.37	97.03
YR-2∶YR-3∶HR-1∶HR-3(1∶1∶1∶1)	48.67	96.02

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