Fouling characteristics of microorganisms in circulating cooling water system

doi:10.19965/j.cnki.iwt.2021-1243

Abstract

Abstract:

Some specific bacteria can grow in the industrial circulating cooling water piping and secrete extracellular polymeric substances（EPS）. The interaction between EPS and circulating cooling water is the main cause of biological fouling in industrial circulating cooling water system. High-throughput biological sequencing was used to analyze the dominant bacteria of biological slime in the circulating cooling water system. EPS secreted by the dominant strains in slime was investigated，the equilibrium adsorption experiment was carried out by mixing EPS with mineral suspension. Based on the traditional chemical analysis，modern analytical technologies including XRD，Raman，EDS and SEM were applied to investigate the minerals formed by biochemical reaction between EPS and ions in circulating cooling water，and the adsorption behavior of EPS on the surface of main minerals. It provided an important theoretical and experimental basis for reducing microbial fouling corrosion. The results showed that when the microorganisms were incubated in circulating cooling water containing a higher concentration of nutrient，Actinomycetes gradually became the dominant bacteria in cooling water. In a neutral environment（pH=7.78） with roughly same pH of circulating cooling water，polysaccharides（EPS-C） and protein（EPS-N） in EPS were more likely to adsorb on Fe₂O₃ surface，while nucleic acid（EPS-P） was more likely to adsorb on SiO₂ surface. The adsorption of EPS on mineral surface was physical adsorption.

Key words: fouling, corrosion, microorganism, extracellular polymeric substances, circulating cooling water system

摘要：

由生长在循环冷却水系统管道中的特异性细菌分泌的胞外聚合物（EPS）与循环冷却水的相互作用是导致工业循环冷却水系统产生生物污垢的主要原因。利用高通量生物测序分析循环冷却水系统中生物黏泥的优势菌种，以优势菌种分泌的EPS为研究对象，将其与矿物悬液混合进行平衡吸附实验。在传统化学分析的基础上，运用XRD、Raman、EDS、SEM等现代仪器分析技术，研究EPS与循环冷却水中离子经生化反应形成的矿物，以及EPS在主要矿物表面的吸附行为，以期为减少微生物污垢腐蚀提供重要的理论和实验依据。结果表明：循环冷却水中的微生物在高营养水平培养条件下，放线菌纲将逐渐成为冷却水中的优势菌；在与循环冷却水pH大致相同的偏中性（pH=7.78）环境下，EPS中的多糖（EPS-C）和蛋白质（EPS-N）更倾向于吸附在Fe₂O₃表面，但EPS中的核酸（EPS-P）更易吸附在SiO₂表面；EPS在矿物表面的吸附为物理吸附。

关键词: 结垢, 腐蚀, 微生物, 胞外聚合物, 循环冷却水系统

CLC Number:

TQ085
X703.1

Qianwei LI, Ran PENG, Yuzhu WANG, Daoqing LIU. Fouling characteristics of microorganisms in circulating cooling water system[J]. Industrial Water Treatment, 2022, 42(10): 77-83.

李倩玮, 彭然, 王雨竹, 刘道庆. 循环冷却水系统中微生物的结垢特性[J]. 工业水处理, 2022, 42(10): 77-83.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2021-1243

https://www.iwt.cn/EN/Y2022/V42/I10/77

Figures/Tables 8

Fig. 1 EDS results of biological slime before equilibrium adsorption

Fig. 2 XRD results of biological slime

Fig. 3 Microbial community in circulating cooling water on class level

Fig. 4 Raman of EPS

Table 1 Adsorption capacity of EPS-C，EPS-N and EPS-P on different mineral surfaces

矿物复合体	EPS-C吸附量/（mg·g^-1）	EPS-N吸附量/（mg·g^-1）	EPS-P吸附量/（mg·g^-1）
EPS-CaCO₃	11.64	3.96	21.51
EPS-Fe₂O₃	19.84	4.24	27.23
EPS-SiO₂	4.48	1.12	31.08

Fig. 5 SEM of EPS-CaCO₃（a），EPS-Fe₂O₃（b） and EPS-SiO₂（c）

Fig. 6 EDS of EPS-CaCO₃，EPS-Fe₂O₃ and EPS-SiO₂

Fig. 7 XRD analysis results of EPS-mineral compounds

References 19

1	刘芳，张桂芝，夏璐，等. 操作条件对循环冷却水系统腐蚀和结垢的影响［J］. 化工进展，2010，29（4）：772-777.
	LIU Fang， ZHANG Guizhi， XIA Lu，et al. Effects of operating conditions on corrosion and fouling in circulating cooling water system［J］. Chemical Industry and Engineering Progress，2010，29（4）：772-777.
2	CHEN Xiao， YANG Qirong， WU Ronghua，et al. Experimental study of the growth characteristics of microbial fouling on sewage heat exchanger surface［J］. Applied Thermal Engineering，2018，128：426-433. doi:10.1016/j.applthermaleng.2017.09.035
3	陆善忠，窦从容. 工业循环冷却水中微生物的危害及控制［J］. 给水排水，2004，30（6）：46-48. doi:10.3969/j.issn.1002-8471.2004.06.015
	LU Shanzhong， DOU Congrong. The harm and control of microbes in industrial recirculating cooling water［J］. Water and Wastewater Engineering，2004，（6）：46-48. doi:10.3969/j.issn.1002-8471.2004.06.015
4	于大禹，尹旭，张静，等. 基于模拟循环冷却装置的微生物污垢形成的影响因素［J］. 化工学报，2011，62（12）：3503-3510. doi:10.3969/j.issn.0438-1157.2011.12.030
	YU Dayu， YIN Xu， ZHANG Jing，et al. Factors affecting microbial fouling formation in simulated recirculating cooling-water system［J］. CIESC Journal，2011，62（12）：3503-3510. doi:10.3969/j.issn.0438-1157.2011.12.030
5	黄元平，武霖，徐志明. 电厂换热设备微生物污垢沉积与腐蚀实验［J］. 热力发电，2015，44（6）：111-116. doi:10.3969/j.issn.1002-3364.2015.06.111
	HUANG Yuanping， WU Lin， XU Zhiming. Experimental study on microbial fouling and corrosion on heat exchangers surface in power plants［J］. Thermal Power Generation，2015，44（6）：111-116. doi:10.3969/j.issn.1002-3364.2015.06.111
6	XU Zhiming， WANG Jingtao， JIA Yuting，et al. Experimental study on microbial fouling characteristics of the plate heat exchanger［J］. Applied Thermal Engineering，2016，108：150-157. doi:10.1016/j.applthermaleng.2016.07.110
7	易哲. 生物缓蚀阻垢制剂在循环冷却水处理中的研究［D］. 重庆：重庆大学，2017.
	YI Zhe. The research of bio-corrosion and scale inhibitor in the treatment of circulating cooling water［D］. Chongqing：Chongqing University，2017.
8	LIU Xiyang， GENG Mingxian， ZHOU Shiyu. Study on the scale and corrosion inhibitor of urban reclaimed water reused in circulating cooling water of power plant［J］. IOP Conference Series：Earth and Environmental Science，2019，227：052061. doi:10.1088/1755-1315/227/5/052061
9	刘梦. 发电厂循环冷却水系统中弗氏柠檬酸杆菌胞外聚合物对不锈钢作用机制的研究［D］. 北京：北京交通大学，2020.
	LIU Meng. The study on the mechanism of action of extracellular polymeric substances of citrobacter freundii on stainless steel in circulating cooling water system of power plant［D］. Beijing：Beijing Jiaotong University，2020.
10	BEECH I B， SUNNER J. Biocorrosion：Towards understanding interactions between biofilms and metals［J］. Current Opinion in Biotechnology，2004，15（3）：181-186. doi:10.1016/j.copbio.2004.05.001
11	CAO Daqi， WANG Xin， WANG Qunhui，et al. Removal of heavy metal ions by ultrafiltration with recovery of extracellular polymer substances from excess sludge［J］. Journal of Membrane Science，2020，606：118103. doi:10.1016/j.memsci.2020.118103
12	JIN Juntao， WU Guangxue， ZHANG Zhenhua，et al. Effect of extracellular polymeric substances on corrosion of cast iron in the reclaimed wastewater［J］. Bioresource Technology，2014，165：162-165. doi:10.1016/j.biortech.2014.01.117
13	陈洋. 钙离子环境下板式换热器微生物特性的实验研究［D］. 吉林：东北电力大学，2015.
	CHEN Yang. Plate heat exchanger fouling characteristics of microorganisms on calcium ion environment［D］. Jilin：Northeast Dianli University，2015.
14	郦和生，王岽，张春原，等. 污水回用时常见离子对细菌生长及控制的影响［J］. 工业水处理，2002，22（7）：24-27. doi:10.11894/1005-829x.2002.22(7).24
	LI Hesheng， WANG Dong， ZHANG Chunyuan，et al. Effect of common ions in effluent on bacteria growth and control in wastewater reuse［J］. Industrial Water Treatment，2002，22（7）：24-27. doi:10.11894/1005-829x.2002.22(7).24
15	林迪. 细菌胞外聚合物在土壤矿物和纳米颗粒表面的吸附及其驱动团聚机制［D］. 武汉：华中农业大学，2018.
	LIN Di. Adsorption and aggregation mechanisms between bacterial extracellular polymeric substances and soil minerals and nanoparticles［D］. Wuhan：Huazhong Agricultural University，2018.
16	李进，黄忠源，张万友，等. 300 MW级火力发电机组循环冷却水阻垢缓蚀剂性能研究［J］.工业水处理，2014，34（10）：17-21. doi:10.11894/1005-829x.2014.34(10).017
	LI Jin， HUANG Zhongyuan， ZHANG Wanyou，et al. Study on the capacities of scale and corrosion inhibitors used for the circulating cooling water in 300 MW thermal power generating units［J］. Industrial Water Treatment，2014，34（10）：17-21. doi:10.11894/1005-829x.2014.34(10).017
17	王金美. 一. 工业循环冷却水系统中冷却水和生物膜微生物群落结构二. 土壤中莠去津降解菌的分离和特性研究［D］. 南京：南京大学，2013.
	WANG Jinmei. 1. Bacterial community structure in cooling water and biofilm in an industrial recirculating cooling water system. 2. Isolation and characterization of atrazine-degrading Arthrobacter sp. wjm3 from an agricultural soil［D］. Nanjing：Nanjing University，2013.
18	周丹妮. 生物促生剂对好氧絮状污泥胞外聚合物性能的影响［D］. 西安：陕西科技大学，2017.
	ZHOU Danni. The effects of bio-stimulant on extracellular polymeric substances performance of activated sludge［D］. Xi’an：Shaanxi University of Science & Technology，2017.
19	PARIKH S J， MUKOME F N D， ZHANG Xiaoming. ATR-FTIR spectroscopic evidence for biomolecular phosphorus and carboxyl groups facilitating bacterial adhesion to iron oxides［J］. Colloids and Surfaces B：Biointerfaces，2014，119：38-46. doi:10.1016/j.colsurfb.2014.04.022

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