Ammonia anaerobic oxidation driven by electrodes in a modified iron anode microbial electrolysis cells

doi:10.19965/j.cnki.iwt.2022-0086

Abstract

Abstract:

In the absence of NO₂ ^- or partial nitrification，anaerobic ammonia oxidizing bacteria（AnAOB） can oxidize NH₄ ⁺-N by using the anodes of microbial electrolytic cells（MECs） as electron acceptors. The electrodes dependent anaerobic ammonium oxidation（E-Anammox） can effectively solve the problem of unstable supply of NO₂ ^- which is electron acceptor in conventional anaerobic ammonium oxidation（Anammox）. However，the commonly used iron anode in the MECs system is prone to corrosion slabbing，which leads to the decrease of electrode conductivity and electron utilization. To solve the corrosion problem of iron anode，the modified iron anode was prepared by coating the common iron anode. The modified iron anode and the common iron anode were loaded with the MECs in the Anammox reaction system. The changes of ammonia oxidation，system impedance value，and microbial community structure during the energized operation were investigated. The results showed that the effect of modified iron anode-MECs reactor（R2） on ammonia oxidation was stronger than that of the common iron anode-MECs reactor（R1），and the NH₄ ⁺-N removal rates of R1 and R2 at the end of the experiment（70 d） were 35.57 mg/（L·d）and 76.72 mg/（L·d），respectively. The impedance value of R1 increased from less than R2 in the early stage to more than R2 in the late stage. Furthermore，the relative abundance of AnAOB in R2 was higher than that in R1，and the relative abundance of iron-reducing bacteria in R1 was significantly higher than that in R2. Thus，it can be seen that the coating modification of iron anode could slow down its corrosion and play as an electron acceptor to achieve anaerobic oxidation of ammonia in the absence of NO₂ ^- supply.

Key words: iron anode, corrosion protection, microbial electrolysis cells, anaerobic ammonium oxidation

摘要：

在没有NO₂ ^-或短程硝化的情况下，厌氧氨氧化菌（AnAOB）可以微生物电解池（MECs）的阳极为电子受体氧化NH₄ ⁺-N，这种电极驱动型厌氧氨氧化（E-Anammox）可有效解决传统厌氧氨氧化（Anammox）电子受体NO₂ ^-供给不稳定的问题。但MECs系统常用的铁阳极易发生腐蚀板结，导致电极导电能力下降、电子利用率降低。针对铁阳极易腐蚀的问题，对普通铁阳极进行包覆改性制备改性铁阳极，将改性铁阳极和普通铁阳极分别与MECs系统耦合加载于Anammox反应体系中，考察了2个反应体系中氨氧化、系统阻抗值、微生物群落结构的变化特征。结果表明，改性铁阳极-MECs反应器（R2）对氨氧化的促进作用强于普通铁阳极-MECs反应器（R1），至实验末期（第70天），R1和R2的NH₄ ⁺-N去除速率分别为35.57 mg/（L·d）和76.72 mg/（L·d）；R1的阻抗值由运行前期的小于R2增大至后期明显大于R2；AnAOB在R2中的相对丰度高于R1，而铁还原菌在R1中的相对丰度明显高于R2。对铁阳极进行包覆制备的改性铁阳极可减缓铁腐蚀，且在没有NO₂ ^-供给的情况下，可作为电子受体实现氨的厌氧氧化。

关键词: 铁阳极, 防腐, 微生物电解池, 厌氧氨氧化

CLC Number:

X703.1

Yawen SHI, Changxun XIONG, Yongli QIN, Yongrong JIANG, Jinbang XIE, Shanshan MO, Libiao QIU. Ammonia anaerobic oxidation driven by electrodes in a modified iron anode microbial electrolysis cells[J]. Industrial Water Treatment, 2022, 42(6): 85-91.

施亚雯, 熊昌勋, 秦永丽, 蒋永荣, 谢锦邦, 莫姗姗, 邱立标. 改性铁阳极微生物电解池中电极驱动的氨厌氧氧化[J]. 工业水处理, 2022, 42(6): 85-91.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2022-0086

https://www.iwt.cn/EN/Y2022/V42/I6/85

Figures/Tables 8

Fig. 1 MECs-UASB reactor

Fig. 2 Tafel curves of modified iron anode and ordinary iron anode

Table 1 Tafel parameters of modified iron anode and ordinary iron anode

阳极类型	E _corr/V	I _corr/A	β
普通铁阳极	-0.379	5.578×10^-7	5.719
改性铁阳极	-0.844	3.635×10^-7	4.969

Fig. 3 The Changes of NH₄ ^＋-N

Fig. 4 AC impedance diagram of reaction systems

Fig. 5 Abundance of samples at phylum level

Fig. 6 Abundance of samples at genus level

Fig. 7 Mechanism of E-Anammox in modified iron anode-MECs

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