Domestication of dye-degrading salt tolerant bacteria and the community structure analysis

doi:10.19965/j.cnki.iwt.2022-0459

Abstract

Abstract:

In order to further improve the treatment efficiency of salinity dye wastewater， NaCl， reduced blue 4 （VB4） and NaCl+VB4 were added into three groups of SBR reactors （R1， R2 and R3）， and their concentration was gradually increased. The treatment effect of activated sludge on simulated wastewater and microbial community structure were investigated under different conditions of salinity and dye concentration. The results showed that the COD removal rate of R1 and R3 started to decrease significantly when the salinity increased to 3%. The average COD removal rate was 95.5% in R2， where only dye was added. The average removal rate of ammonia nitrogen was above 90% in all reactors. The stability of the system decreased with the increase of salinity and dye concentration. The decolorization performance of sludge on dyes began to decline when the salinity and dye mass concentration reached 1% and 40 mg/L， respectively. The results of high-throughput sequencing analysis showed that the diversity of activated sludge microbial community decreased with the increase of salinity and dye， and the functional bacteria for degrading COD and dye were mainly affected by the salinity of the system， while the ammonia-nitrogen degrading bacteria were not affected by environmental changes. During the operation of each reactor， norank-f-norank-o-saccharimonadales， Propioniciclava and Micropruina had the highest abundance and showed better tolerance to salinity and dye， and were the dominant bacteria in the treatment system of saline printing and dyeing wastewater.

Key words: dye degradation, salt tolerance, activated sludge, domestication, microorganism

摘要：

为进一步提升对含盐染料废水的处理效率，在3组SBR反应器（R1、R2、R3）中分别投加NaCl、还原蓝4（VB₄）、NaCl+VB₄，并逐步提高其投加浓度，探究在不同盐度和染料浓度的驯化条件下，活性污泥对模拟废水的处理效果以及微生物群落的组成结构。结果表明：盐度增加至3%时，R1和R3的COD去除率开始大幅降低；在仅投加染料的反应器R2中，COD的平均去除率为95.5%；各反应器的氨氮平均去除率在90%以上。随着盐度和染料浓度的提升，系统稳定性下降。盐度和染料质量浓度分别达到1%、40 mg/L时，污泥对染料的脱色性能开始下降。高通量测序分析结果表明，随着盐度和染料的增加，活性污泥的微生物群落多样性降低，降解 COD及染料的功能菌群主要受系统含盐量的影响，氨氮降解菌群受环境变化的影响不大。在各反应器运行过程中，norank-f-norank-o-saccharimonadales、Propioniciclava、Micropruina3个菌属的丰度最高，对盐和染料表现出较好的耐受性，为含盐印染废水处理系统的优势菌群。

关键词: 染料降解, 耐盐, 活性污泥, 驯化, 微生物

CLC Number:

X703.1

Yanli MA, Haihong LI, Qi BIN. Domestication of dye-degrading salt tolerant bacteria and the community structure analysis[J]. Industrial Water Treatment, 2023, 43(4): 78-84.

马艳丽, 李海红, 宾齐. 耐盐染料降解菌群驯化及其微生物群落分析[J]. 工业水处理, 2023, 43(4): 78-84.

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https://www.iwt.cn/EN/Y2023/V43/I4/78

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References 21

1	VIKRANT K， GIRI B S， RAZA N，et al. Recent advancements in bioremediation of dye：Current status and challenges［J］. Bioresource Technology，2018，253：355-367. doi:10.1016/j.biortech.2018.01.029
2	GHAEDI A M， VAFAEI A. Applications of artificial neural networks for adsorption removal of dyes from aqueous solution：A review［J］. Advances in Colloid and Interface Science，2017，245：20-39. doi:10.1016/j.cis.2017.04.015
3	KEMPF B， BREMER E. Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments［J］. Archives of Microbiology，1998，170（5）：319-330. doi:10.1007/s002030050649
4	GUADIE A， TIZAZU S， MELESE M，et al. Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake［J］. Biotechnology Reports，2017，15：92-100. doi:10.1016/j.btre.2017.06.007
5	GUO Guang， HAO Jiuxiao， TIAN Fang，et al. Decolorization of Metanil Yellow G by a halophilic alkalithermophilic bacterial consortium［J］. Bioresource Technology，2020，316：123923. doi:10.1016/j.biortech.2020.123923
6	董仲生. 《还原蓝RSN（C. I. 还原蓝4）》国家标准简介［J］. 中国石油和化工标准与质量，2009，29（11）：4-8.
	DONG Zhongsheng. Introduction to the national standard of Vat blue RSN（C.I. Vat blue 4）［J］. China Petroleum and Chemical Standard and Quality，2009，29（11）：4-8.
7	赵凯峰，王淑莹，叶柳，等. NaCl盐度对耐盐活性污泥沉降性能及脱氮的影响［J］. 环境工程学报，2010，4（3）：570-574.
	ZHAO Kaifeng， WANG Shuying， YE Liu，et al. Effects of salinity on salt-tolerant activated sludge settling performance and nitrogen removal［J］. Chinese Journal of Environmental Engineering，2010，4（3）：570-574.
8	王子超. 盐度和重金属对序批式生物反应器性能及微生物群落结构影响的研究［D］. 青岛：中国海洋大学，2014. doi:10.1080/09593330.2015.1019932
	WANG Zichao. Effects of salinity and heavy metals on the performance and microbial community structure of sequencing batch bioreactor［D］. Qingdao：Ocean University of China，2014. doi:10.1080/09593330.2015.1019932
9	冯叶成，占新民，文湘华，等. 活性污泥处理系统耐含盐废水冲击负荷性能［J］. 环境科学，2000，21（1）：106-108. doi:10.3321/j.issn:0250-3301.2000.01.026
	FENG Yecheng， ZHAN Xinmin， WEN Xianghua，et al. Effects of shock salinity loading on activated sludge treatment system［J］. Chinese Journal of Enviromental Science，2000，21（1）：106-108. doi:10.3321/j.issn:0250-3301.2000.01.026
10	黄雅楠，王晓慧，曹琦，等. 高通量测序技术分析猪粪堆肥过程中微生物群落结构变化［J］. 微生物学杂志，2018，38（5）：21-26. doi:10.3969/j.issn.1005-7021.2018.05.004
	HUANG Yanan， WANG Xiaohui， CAO Qi，et al. Analysis of microbial community changes in pig excrement during compost process based on high-throughput sequencing technology［J］. Journal of Microbiology，2018，38（5）：21-26. doi:10.3969/j.issn.1005-7021.2018.05.004
11	CHEN Weiming， WANG Fan， ZENG Lin，et al. Bioremediation of petroleum-contaminated soil by semi-aerobic aged refuse biofilter：Optimization and mechanism［J］. Journal of Cleaner Production，2021，294：125354. doi:10.1016/j.jclepro.2020.125354
12	HAMODA M F， AL-ATTAR I M S. Effects of high sodium chloride concentrations on activated sludge treatment［J］. Water Science and Technology，1995，31（9）：61-72. doi:10.2166/wst.1995.0345
13	TIAN Renmao， NING Daliang， HE Zhili，et al. Small and mighty：Adaptation of superphylum Patescibacteria to groundwater environment drives their genome simplicity［J］. Microbiome，2020，8（1）：51. doi:10.1186/s40168-020-00825-w
14	刘昊明，罗志浩，欧阳二明. 改进型SBBR中SND启动及微生物群落结构研究［J］. 工业水处理，2021，41（3）：67-71.
	LIU Haoming， LUO Zhihao， OUYANG Erming. Start-up of simultaneous nitrification and denitrification and the microbial community structure in the improved SBBR［J］. Industrial Water Treatment，2021，41（3）：67-71.
15	SANG Yizhou， LIN Aiguo， LIU Xinliang. Simultaneous removal of carbon，nitrogen and phosphorus from hypersaline wastewater by bioaugmented intermittently aerated biological filter（IABF）［J］. Water and Environment Journal，2019，34：19-28. doi:10.1111/wej.12500
16	CHEN Weiming， WANG Fan， ZENG Lin，et al. Bioremediation of petroleum-contaminated soil by semi-aerobic aged refuse biofilter：Optimization and mechanism［J］. Journal of Cleaner Production，2021，294：125354. doi:10.1016/j.jclepro.2020.125354
17	陈祥瑞，杜强强，韩文杰，等. 基于纯膜MBBR的紧凑型污水处理BFM中试基质转化特性［J］. 环境工程学报，2021，15（11）：3741-3756. doi:10.12030/j.cjee.202107149
	CHEN Xiangrui， DU Qiangqiang， HAN Wenjie，et al. Pilot test on the treatment of medium-concentration domestic sewage in Northern China by BFM process based on pure MBBR［J］. Chinese Journal of Environmental Engineering，2021，15（11）：3741-3756. doi:10.12030/j.cjee.202107149
18	CHEN Chunmao， MING Jie， YOZA B A，et al. Characterization of aerobic granular sludge used for the treatment of petroleum wastewater［J］. Bioresource Technology，2019，271：353-359. doi:10.1016/j.biortech.2018.09.132
19	古柏铭，金春姬，温淳，等. 提盐速率对序批式生物反应器性能和微生物群落结构的影响［J］. 环境科学，2021，42（7）：3413-3421.
	GU Baiming， JIN Chunji， WEN Chun，et al. Effect of rate of salinity increase on the performance and microbial community structure of sequencing batch reactors［J］. Environmental Science，2021，42（7）：3413-3421.
20	杨开明，母宣贻，赵梓君，等. 多级AO工艺处理酿造废水效能及微生物群落分析［J］. 工业水处理，2021，41（11）：112-119.
	YANG Kaiming， MU Xuanyi， ZHAO Zijun，et al. Efficacy and microbial community analysis of multi-stage AO process for brewing wastewater treatment［J］. Industrial Water Treatment，2021，41（11）：112-119.
21	CHEN Rui， YAO Junqin， AILIJIANG N，et al. Abundance and diversity of nitrogen-removing microorganisms in the UASB-anammox reactor［J］. PLoS One，2019，14（4）：e0215615. doi:10.1371/journal.pone.0215615

盐度/%	0	0.5	1.0	2.0	3.0
SV₃₀/%	78.4	75.9	68.9	63.8	54.2
MLSS/（g·L^-1）	7.82	7.59	6.83	7.12	9.76
SVI/（mL·g^-1）	100.22	99.97	100.78	89.72	68.91

盐度/%	0	0.5	1.0	2.0	3.0
SV₃₀/%	78.4	75.9	68.9	63.8	54.2
MLSS/（g·L^-1）	7.82	7.59	6.83	7.12	9.76
SVI/（mL·g^-1）	100.22	99.97	100.78	89.72	68.91

染料质量浓度/（mg·L^-1）	0	20	40	60	80
SV₃₀/%	76.4	70.9	71.7	68.2	62.5
MLSS/（g·L^-1）	7.65	7.92	7.23	7.54	9.35
SVI/（mL·g^-1）	99.82	89.55	99.24	90.51	70.13

染料质量浓度/（mg·L^-1）	0	20	40	60	80
SV₃₀/%	76.4	70.9	71.7	68.2	62.5
MLSS/（g·L^-1）	7.65	7.92	7.23	7.54	9.35
SVI/（mL·g^-1）	99.82	89.55	99.24	90.51	70.13

盐度/%	0	0.5	1.0	2.0	3.0
染料质量浓度/（mg·L^-1）	0	20	40	60	80
SV₃₀/%	78.4	70.33	62.35	45.75	30.83
MLSS/（g·L^-1）	7.03	7.09	6.34	6.03	5.63
SVI/（mL·g^-1）	111.57	99.17	98.46	75.42	54.66

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