Treatment of protein-containing organic wastewater by “electrical” collaboration between dissimilatory iron reduction and methanogenesis

doi:10.19965/j.cnki.iwt.2024-0453

Abstract

Abstract:

Aiming at the problems of poor methane performance and acids accumulation in the anaerobic digestion process of protein-containing organic wastewater, Fe₃O₄ was used as an exogenous electronic mediator to construct a magnetite-mediated anaerobic digestion system of protein-containing organic wastewater. The operational efficiency of the system was researched, the physiological characteristics of sludge in the reactor were analyzed, and the response mechanism of community succession to different Fe₃O₄ doses was explored. The results showed that the maximum optimization of anaerobic fermentation could be achieved under experimental conditions with the Fe₃O₄ dosage of 40 mg/g in unit VSS. Compared with the control group without Fe₃O₄, the degradation rate of soluble chemical oxygen demand(SCOD) and cumulative CH₄ production increased by (27.23±4.16)% and (26.50±3.25)%, respectively, while the cumulative CO₂ production decreased by (82.08±2.43)%. The analysis of physiological characteristics of sludge showed that the addition of Fe₃O₄ could promote sludge aggregation and enhance electron transfer ability. The analysis of microbial community changes showed that the addition of 40 mg/g Fe₃O₄could promote the enrichment of syntrophic genus Syntrophomonas, iron-reducing bacteria genus Clostridium, and acetoclastic archaea Methanosaeta. Thus, an electron transfer mode of dissimilar iron reduction coupled with methanation could be established, thereby improving the efficiency of interspecific electron transfer and enhancing the degradation of pollutants in protein organic wastewater and the final methanation process.

Key words: anaerobic digestion, protein-containing organic wastewater, magnetite, microbial population, direct interspecific electron transfer

摘要：

针对蛋白质有机废水厌氧消化过程中出现的产甲烷性能不佳、易于酸积累等问题，利用Fe₃O₄作为外源电子介体构建Fe₃O₄介导的蛋白质有机废水厌氧消化体系，考察体系的运行效能，分析反应器中污泥的生理特征，并探究群落演替对不同Fe₃O₄剂量的响应机制。研究结果表明，实验条件下每克VSS中添加40 mg的Fe₃O₄能够实现体系厌氧发酵性能的最大优化，相较于未添加Fe₃O₄的对照组，溶解性化学需氧量（SCOD）降解率与累积CH₄产量分别提高了（27.23±4.16）%和（26.50±3.25）%，累积CO₂产量降低了（82.08±2.43）%。污泥生理特征分析表明，Fe₃O₄的添加能够促进污泥团聚，增强电子转移能力。微生物群落变化分析表明，添加40 mg/g的Fe₃O₄可促进互营菌属Syntrophomonas、铁还原菌属Clostridium和嗜乙酸型产甲烷古菌Methanosaeta的富集，从而形成异化铁还原耦合甲烷化的电子传递模式，提升种间电子传递效率，强化蛋白质有机废水中污染物的降解以及最终甲烷化的过程。

关键词: 厌氧消化, 蛋白质有机废水, 磁铁矿, 微生物种群, 直接种间电子传递

CLC Number:

X52
X703.1

Xinwei ZHOU, Xiangzhuang FU, Mengxia GUO, Wen XU, Dexin WANG. Treatment of protein-containing organic wastewater by “electrical” collaboration between dissimilatory iron reduction and methanogenesis[J]. Industrial Water Treatment, 2025, 45(6): 167-178.

周欣玮, 符祥壮, 郭梦霞, 徐文, 王德欣. 异化铁还原“电”耦合甲烷化处理蛋白质有机废水[J]. 工业水处理, 2025, 45(6): 167-178.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2024-0453

https://www.iwt.cn/EN/Y2025/V45/I6/167

Figures/Tables 10

Fig.1 Schematic diagram of reaction device

Fig.2 Diagram of high H₂ partial pressure suppression experimental apparatus

Fig.3 Changes in SCOD of wastewater in each reactor during AD process

Fig.4 Changes of Fe²⁺ concentration in additional groups during AD process

Fig.5 Changes of VFAs concentration（a-e） and pH（f） in each reactor during AD process

Fig.6 Changes of cumulative CH₄ production（a） and cumulative CO₂ production（b） in each reactor during AD process

Fig.7 Characterization of sludge morphology and analysis of physicochemical properties

Fig.8 Changes of SCOD at high H₂ partial pressure

Fig.9 The relative abundance of bacteria and archaea in experimental group sludge at phylum and genus levels

Fig.10 Schematic diagram of electron transfer mechanism in IHT and DIR “electric” coupling methane production

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