Abstract: The SRB immobilized particles have been prepared based on the immobilization technology of microorganisms and used for treating acid mine drainage. On the basis of single-factor experiments,the response surface methodology has been used for the optimization of iron scrap type and ratio in the substrates of SRB immobilized particles. The results show that the best ratio of scrap iron are as follows:the type of raw iron is pig scraps,the dosage of pig scraps is 0.6 g when the mass of PVA,sodium alginate,medical stone and corn cobs are 0.9,0.05,0.3,and 0.5 g,respectively,and the particle size is 0.05 to 0.075 mm. Under these conditions,the actual SO₄^2- removing rate of immobilized particles prepared is 82.17%(predicted value is 83.414%). The results of variance analysis of response surfaces show that the fitting effect of obtained regression model is comparatively good,and the interactions among various influencing factors are very obvious.

Key words: ratio of scrap iron, sulfate reducing bacteria (SRB), immobilized particles, acidic coal mine wastewater

摘要： 基于微生物固定化技术制备SRB固定化颗粒，用其处理煤矿酸性废水。在单因素实验基础上，采用响应曲面法优化了SRB固定化颗粒基质中的铁屑种类和配比。结果表明，固定化颗粒的最佳铁屑种类为生铁屑，在PVA、海藻酸钠、麦饭石、玉米芯质量分别为0.9、0.05、0.3、0.5 g的条件下，最佳铁屑投加量为0.6 g，铁屑粒径为0.05~0.075 mm。在此条件下制备的固定化颗粒对废水的实际SO₄^2-去除率为82.17%（预测值为83.414%）。响应曲面方差分析结果表明，所得回归模型拟合效果较好，各影响因素间的交互作用均非常明显。

关键词: 铁屑配比, 硫酸盐还原菌, 固定化颗粒, 煤矿酸性废水

CLC Number: 

• X523

• X703