Adsorption mechanism of anaerobic granular sludge flora structure to Sb（Ⅲ） in water

doi:10.19965/j.cnki.iwt.2021-0653

Abstract

Abstract:

According to the different physiological habits，the microbes of the anaerobic granular sludge can be divided into two major groups of methanogenic bacteria and non-methanogenic bacteria. In order to explore the mechanism of the two microbial groups on the removal of Sb（Ⅲ） pollution in water，the interaction process of EPS（extracellular polymer） of anaerobic granular sludge and its inner core microorganisms and Sb（Ⅲ） in water was studied. The results showed that its inner core microorganism was the main body to remove Sb（Ⅲ） pollution in water. The pH not only affected the physiological activity of anaerobic microorganisms of different flora，but also had a greater impact on the protonation rate of the surface functional groups of the microorganisms，thereby affecting the adsorption of Sb（Ⅲ） by anaerobic granular sludge microorganisms. The removal of Sb（Ⅲ） by methanogenic bacteria was mainly based on biomass precipitation，and its adsorption capacity was greater than that of non-methanogenic bacteria. The interaction mode of non-methanogenic bacteria and Sb（Ⅲ） in water was mainly combined with biomass. FTIR，XPS and XRD characterizations proved that no matter what kind of anaerobic granular sludge flora，there were a large number of organic structures such as polysaccharides，proteins and carboxylic acids that could bind to Sb（Ⅲ） in water. In addition，Fe and P accumulated in anaerobic granular sludge could form complexes with Sb and deposit in microbial cells，especially a large amount of biological iron Fe²⁺ reacted with Sb（Ⅲ） to form FeOSbO₃ co-precipitate deposits to obtain the removal effect of Sb（Ⅲ） in water.

Key words: anaerobic granular sludge, antimony pollution control, microbial flora, methanogenic bacteria, adsorption mechanism

摘要：

根据生理生活习性的不同可将厌氧颗粒污泥的组成微生物分为产甲烷菌群及非产甲烷菌群两大菌群。为探究这两大菌群微生物对去除水中Sb（Ⅲ）污染的作用机理，研究了厌氧颗粒污泥的EPS（胞外聚合物）及其内核微生物与水中Sb（Ⅲ）的作用过程。结果表明，其内核微生物是去除水中Sb（Ⅲ）污染的主体；pH不仅影响不同菌群厌氧微生物的生理活性，同时还对微生物表面官能团的质子化率有较大的影响，从而影响厌氧颗粒污泥微生物对Sb（Ⅲ）的吸附；产甲烷菌群对Sb（Ⅲ）的去除以生物质沉淀方式为主，其吸附容量大于非产甲烷菌群，非产甲烷菌群与水中Sb（Ⅲ）的作用方式主要以生物质结合为主。FTIR、XPS及XRD表征证明，无论是何种厌氧颗粒污泥菌群，其表面均大量存在能与水中Sb（Ⅲ）结合的多糖、蛋白质和羧酸等有机结构。另外，厌氧颗粒污泥内部积累的Fe、P可与Sb生成络合物并沉积在微生物细胞内，尤其是大量存在的生物铁Fe²⁺可与Sb（Ⅲ）发生络合反应生成FeOSbO₃共沉淀物，从而获得对水中Sb（Ⅲ）的去除效果。

关键词: 厌氧颗粒污泥, 锑污染治理, 微生物菌群, 产甲烷菌群, 吸附机理

CLC Number:

Yufei WANG,Xifeng WANG,Zhentao WU,Renjian DENG,Xueqin WANG. Adsorption mechanism of anaerobic granular sludge flora structure to Sb（Ⅲ） in water[J]. Industrial Water Treatment, 2022, 42(3): 82-89.

王宇飞,王西峰,吴振涛,邓仁健,王雪琴. 厌氧颗粒污泥菌群结构对水中Sb（Ⅲ）的吸附机理[J]. 工业水处理, 2022, 42(3): 82-89.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2021-0653

https://www.iwt.cn/EN/Y2022/V42/I3/82

Figures/Tables 8

Fig.1 Removal rate of Sb（Ⅲ） by granular sludge kernel microorganisms and their EPS（A：Mainly methanogen bacteria；B：Mainly non-methanogenic bacteria）

Table 1 EPS components of anaerobic granular sludge microorganisms of different flora

组分	产甲烷菌群		非产甲烷菌群
组分	LB-EPS	TB-EPS	LB-EPS	TB-EPS
多糖/（mg·L^-1）	106.7	175.1	171.6	227.6
蛋白质/（mg·L^-1）	144.3	286.1	267.3	371.1

Fig. 2 Adsorption of Sb（Ⅲ） by the inner core（A：Mainly methanogenic bacteria；B：Mainly non-methanogenic bacteria）

Fig.3 Adsorption of Sb（Ⅲ） by the composition of inner core microorganisms（A：Mainly methanogenic bacteria；B：Mainly non-methanogenic bacteria）

Fig. 4 FTIR spectrum

Fig.5 XPS spectrum

Table 2 Percentage of C1s peak area at the interface of anaerobic granular sludge

污泥种类	峰面积百分比
	286.1 eV	287.9 eV
	C—OH	C $? ?????$ O
以产甲烷菌群为主的厌氧颗粒污泥	26.51	6.79
以产甲烷菌群为主的厌氧颗粒污泥吸附 Sb（Ⅲ）后	28.28	7.616
以非产甲烷菌群为主的厌氧颗粒污泥	26.54	5.87
以非产甲烷菌群为主的厌氧颗粒污泥吸附Sb（Ⅲ）后	31.11	6.51

Table 2 Percentage of C1s peak area at the interface of anaerobic granular sludge

污泥种类	峰面积百分比
	286.1 eV	287.9 eV
	C—OH	C $? ?????$ O
以产甲烷菌群为主的厌氧颗粒污泥	26.51	6.79
以产甲烷菌群为主的厌氧颗粒污泥吸附 Sb（Ⅲ）后	28.28	7.616
以非产甲烷菌群为主的厌氧颗粒污泥	26.54	5.87
以非产甲烷菌群为主的厌氧颗粒污泥吸附Sb（Ⅲ）后	31.11	6.51

Fig.6 XRD patterns of anaerobic granular sludge composed of different bacteria after adsorption of Sb（A：Mainly methanogenic bacteria；B：Mainly non-methanogenic bacteria）

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