Rapid start-up of MBBR partial nitrification by free nitrite pretreatment

doi:10.19965/j.cnki.iwt.2022-0591

Abstract

Abstract:

Partial nitrification process has the advantages of saving nitrification oxygen demand and denitrification carbon source， which is the focus of current research in the field of water treatment. The study of free nitrite （FNA） pretreatment method to realize start-up and stable operation of partial nitrification is in line with the concept of energy saving and low carbon. Six identical moving bed biofilm reactors （MBBR） were pretreated with FNA at mass concentrations of 0， 3.2， 6.4， 9.6， 12.8， and 16.0 mg/L， respectively， and the biofilms were immersed for 12 h.The untreated reactor was used as a control. The effects of FNA concentration on functional microbial community and the operational stability of partial nitrification under optimal conditions were investigated. The experimental results showed that pretreatment at 25 ℃ and FNA mass concentration of 6.4 mg/L for 12 h could reduce nitrite oxidizing bacteria （NOB） and maintain the activity of ammonia oxidizing bacteria （AOB）. High-throughput sequencing and qPCR results showed that the relative abundance and abundance of NOB decreased significantly after FNA pretreatment. The biofilm was pretreated under optimal conditions of FNA and inoculated in MBBR to verify its operational stability. The experimental results showed that the pretreatment of biofilm by FNA could achieve stable partial nitrification and maintain the nitrite accumulation rate stably above 85%.

Key words: free nitrite acid, biofilm, partial nitrification

摘要：

短程硝化工艺具有节约硝化需氧量和反硝化碳源等优势，是目前水处理领域的研究重点。研究游离亚硝酸（FNA）预处理法实现短程硝化的快速启动和稳定运行符合节能低碳的理念。在6个相同的移动床生物膜反应器（MBBR）中分别投加质量浓度为0、3.2、6.4、9.6、12.8、16.0 mg/L的FNA，对生物膜浸泡12 h进行预处理，并以预处理前的反应器作为对照，研究FNA预处理浓度对功能微生物群落的影响，以及最佳条件下短程硝化的运行稳定性。实验结果表明，25 ℃、FNA质量浓度为6.4 mg/L、预处理12 h，是降低亚硝酸盐氧化菌（NOB）并维持氨氧化菌（AOB）活性的最佳条件。高通量测序和qPCR结果显示，经过FNA预处理后，NOB的相对丰度和丰度均显著减少。在FNA最佳条件下预处理生物膜，并将其接种在MBBR中，验证其运行稳定性。实验结果表明，FNA预处理生物膜可实现稳定的短程硝化，使亚氮积累率稳定维持在85%以上。

关键词: 游离亚硝酸, 生物膜, 短程硝化

CLC Number:

X703

Fan DING, Dongbo LIANG, Dongyue LI, Peng LI, Jun LI, Xiaoqiu CHEN. Rapid start-up of MBBR partial nitrification by free nitrite pretreatment[J]. Industrial Water Treatment, 2023, 43(4): 113-120.

丁凡, 梁东博, 李东岳, 李朋, 李军, 陈筱秋. 游离亚硝酸预处理实现MBBR短程硝化的快速启动[J]. 工业水处理, 2023, 43(4): 113-120.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2022-0591

https://www.iwt.cn/EN/Y2023/V43/I4/113

Figures/Tables 9

References 24

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项目	用量	项目	用量
NH₄Cl	230 mg/L	FeCl₃·6H₂O	1 500 mg/L
CH₃COONa	384.6 mg/L	H₃BO₃	150 mg/L
CaCl₂	4 mg/L	CuSO₄·5H₂O	50 mg/L
MgSO₄·7H₂O	40 mg/L	KI	150 mg/L
KH₂PO₄	40 mg/L	MnCl₂·4H₂O	110 mg/L
微量元素	0.6 mL/L	ZnSO₄·7H₂O	120 mL/L
—	—	CoCl₂·6H₂O	150 mg/L
—	—	Na₂MoO₄·2H₂O	60 mg/L

项目	用量	项目	用量
NH₄Cl	230 mg/L	FeCl₃·6H₂O	1 500 mg/L
CH₃COONa	384.6 mg/L	H₃BO₃	150 mg/L
CaCl₂	4 mg/L	CuSO₄·5H₂O	50 mg/L
MgSO₄·7H₂O	40 mg/L	KI	150 mg/L
KH₂PO₄	40 mg/L	MnCl₂·4H₂O	110 mg/L
微量元素	0.6 mL/L	ZnSO₄·7H₂O	120 mL/L
—	—	CoCl₂·6H₂O	150 mg/L
—	—	Na₂MoO₄·2H₂O	60 mg/L

样品	序列条数	覆盖率	丰富度		多样性
样品	序列条数	覆盖率	Ace	Chao	Shannon	Simpson
Y0	333 761	0.996 69	4 563.598 033	4 113.238 724	4.083 585	0.059 985
F0	359 690	0.997 07	4 158.047 047	3 791.937 318	4.257 852	0.038 332
F1	247 255	0.996 15	4 177.460 893	3 757.224 359	4.277 002	0.036 953
F2	340 835	0.996 92	4 135.286 769	3 716.411 940	4.428 967	0.031 884
F3	258 586	0.996 49	3 579.876 211	3 192.920 578	4.355 997	0.034 049
F4	241 738	0.996 24	3 591.310 816	3 195.820 702	4.332 972	0.037 957
F5	322 088	0.997 05	3 836.082 237	3 473.305 398	4.012 956	0.070 853

样品	序列条数	覆盖率	丰富度		多样性
样品	序列条数	覆盖率	Ace	Chao	Shannon	Simpson
Y0	333 761	0.996 69	4 563.598 033	4 113.238 724	4.083 585	0.059 985
F0	359 690	0.997 07	4 158.047 047	3 791.937 318	4.257 852	0.038 332
F1	247 255	0.996 15	4 177.460 893	3 757.224 359	4.277 002	0.036 953
F2	340 835	0.996 92	4 135.286 769	3 716.411 940	4.428 967	0.031 884
F3	258 586	0.996 49	3 579.876 211	3 192.920 578	4.355 997	0.034 049
F4	241 738	0.996 24	3 591.310 816	3 195.820 702	4.332 972	0.037 957
F5	322 088	0.997 05	3 836.082 237	3 473.305 398	4.012 956	0.070 853

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