Study on VFAs production of sludge fermentation with the immobilization of lysozyme on Noria-PEI modified PVA carrier

doi:10.19965/j.cnki.iwt.2023-1101

Abstract

Abstract:

Sludge anaerobic digestion promoted by enzymes has the advantages of mild reaction, high efficiency and environmental protection etc. However, the difficulty of recovery and poor catalytic stability seriously restrict its application. Enzyme immobilization is the key to solve the above problems. A new type of polyvinyl alcohol(PVA) carrier was prepared by Noria and polyethylenimide(PEI) co-deposition. The effect of lysozyme immobilized with Noria-PEI modified PVA carriers on the efficiency of sludge anaerobic fermentation for VFAs production was studied. The results showed that immobilized lysozyme could enhance sludge fermentation to promote SCOD, and SCOD reached the highest concentration of 2 892.4 mg/L within 36 h, which was 3.4 times higher than in the blank(day 6). VFAs concentration peaked at 2 130.8 mg/L on day 4, mainly comprised of acetic acid and propionic acid, accounting for 61.8%-80.7%. The average particle size and specific surface area of sludge in the immobilized enzyme system were 42.957 μm and 0.413 m²/g, indicating that immobilized enzyme enhanced the hydrolysis of granular sludge. Microbial community analysis showed that the hydrolytic acid-producing phyla of Firmicutes, Proteobacteria and Bacteroides accounted for high proportion in the immobilized enzyme system. At the genus level, Macellibacteroides, Petrimona, Lactobacillu, and Clostridium_sensu_stricto_1 were the main genera, which revealed the mechanism of enhanced VFAs production by the immobilized enzyme at microscopic level.

Key words: noria, polyvinyl alcohol carrier, immobilized enzyme, residual sludge, volatile fatty acids

摘要：

生物酶促进污泥厌氧消化具有反应温和、高效环保等优点，然而回收困难和催化稳定性差严重制约其应用，固定化酶是解决上述问题的关键。采用水轮酚（Noria）和聚乙烯亚胺（PEI）共沉积法改性聚乙烯醇（PVA）载体，并研究了Noria-PEI改性PVA载体固定溶菌酶对污泥厌氧发酵产挥发性脂肪酸（VFAs）效能的影响。结果表明，固定化溶菌酶可强化污泥发酵提高SCOD，在36 h内SCOD最高浓度达到2 892.4 mg/L，相比空白组提升3.4倍（第6天），VFAs质量浓度在第4天达到峰值2 130.8 mg/L，乙酸和丙酸占比达到61.8%~80.7%。固定酶系统中污泥的平均粒径为42.957 μm，比表面积为0.413 m²/g，固定化溶菌酶强化了颗粒态污泥水解。微生物群落结构分析显示，在固定酶系统中Firmicutes、Proteobacteria和Bacteroides等水解产酸菌门占比较高；在属水平上，以Macellibacteroides、Petrimonas、Lactobacillus和Clostridium_sensu_stricto_1菌属为主，从微观层面上解释了固定酶强化污泥发酵产酸机理。

关键词: 水轮酚, 聚乙烯醇载体, 固定酶, 剩余污泥, 挥发性脂肪酸

CLC Number:

X703

Haolong WANG, Enze ZHANG, Zicheng WANG, Yuhan ZHAO, Jun NAN, Yanmin SUN, Aijing MA, Lishan ZHOU, Liqiang LIU, Xiang CAI, Xingyu DUAN. Study on VFAs production of sludge fermentation with the immobilization of lysozyme on Noria-PEI modified PVA carrier[J]. Industrial Water Treatment, 2024, 44(4): 155-163.

王昊龙, 张恩泽, 王梓诚, 赵瑜涵, 南军, 孙彦民, 马爱静, 周立山, 刘丽强, 蔡巷, 段兴宇. Noria-PEI改性PVA载体固定化溶菌酶用于污泥发酵产VFAs研究[J]. 工业水处理, 2024, 44(4): 155-163.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2023-1101

https://www.iwt.cn/EN/Y2024/V44/I4/155

Figures/Tables 10

Fig.1 The synthesis of Noria

Fig.2 FT-IR，XRD and ¹H NMR of Noria

Fig.3 XPS of PVA and mPVA （a） and N 1 s core-level of mPVA （b）

Fig.4 Original PVA carrier and modified mPVA carrier

Fig.5 The variation of SCOD with time in each system

Fig.6 The variation of polysaccharide （a） and protein （b） with time in each system

Fig.7 The variation of VFAs with time in each system

Fig.8 Particle size distribution of sludge

Fig.9 Top 20 phyla of microbial communities

Fig.10 Top 20 genera of microbial communities

References 35

1	王莉，何蓉，雷海涛. 城镇污水处理厂污泥处理处置技术现状综述［J］. 净水技术，2022，41（11）：16-21，69.
	WANG Li， HE Rong， LEI Haitao. General review of sludge treatment and disposal technology for urban WWTP［J］. Water Purification Technology，2022，41（11）：16-21，69.
2	戴晓虎，侯立安，章林伟，等. 我国城镇污泥安全处置与资源化研究［J］. 中国工程科学，2022，24（5）：145-153. doi:10.15302/j-sscae-2022.05.017
	DAI Xiaohu， HOU Li’an， ZHANG Linwei，et al. Safe disposal and resource recovery of urban sewage sludge in China［J］. Strategic Study of CAE，2022，24（5）：145-153. doi:10.15302/j-sscae-2022.05.017
3	WU Boran， DAI Xiaohu， CHAI Xiaoli. Critical review on dewatering of sewage sludge：Influential mechanism，conditioning technologies and implications to sludge re-utilizations［J］. Water Research，2020，180：115912. doi:10.1016/j.watres.2020.115912
4	KHANH NGUYEN V， KUMAR CHAUDHARY D， HARI DAHAL R，et al. Review on pretreatment techniques to improve anaerobic digestion of sewage sludge［J］. Fuel，2021，285：119105. doi:10.1016/j.fuel.2020.119105
5	LIU Xiaoguang， WANG Qian， TANG Yuanzhi，et al. Hydrothermal pretreatment of sewage sludge for enhanced anaerobic digestion：Resource transformation and energy balance［J］. Chemical Engineering Journal，2021，410：127430. doi:10.1016/j.cej.2020.127430
6	GUO Yiping， KIM S H， SUNG S H，et al. Effect of ultrasonic treatment of digestion sludge on bio-hydrogen production from sucrose by anaerobic fermentation［J］. International Journal of Hydrogen Energy，2010，35（8）：3450-3455. doi:10.1016/j.ijhydene.2010.01.090
7	JIANG Xinbai， CHEN Dan， MU Yang，et al. Electricity-stimulated anaerobic system（ESAS） for enhanced energy recovery and pollutant removal：A critical review［J］. Chemical Engineering Journal，2021，411：128548. doi:10.1016/j.cej.2021.128548
8	HUANG Xiao， DONG Wenyi， WANG Hongjie，et al. Role of acid/alkali-treatment in primary sludge anaerobic fermentation：Insights into microbial community structure，functional shifts and metabolic output by high-throughput sequencing［J］. Bioresource Technology，2018，249：943-952. doi:10.1016/j.biortech.2017.10.104
9	刘改革. 溶菌酶与鼠李糖脂联用强化污泥破解效能及机制研究［D］. 哈尔滨：哈尔滨工业大学，2019. doi:10.1016/j.biortech.2019.121703
	LIU Gaige. Performance and mechanism of waste activated sludge decomposition enhanced by the combined lysozyme and rhamnolipid［D］. Harbin：Harbin Institute of Technology，2019. doi:10.1016/j.biortech.2019.121703
10	LIU Gaige， WANG Ke， LI Xiangkun，et al. Enhancement of excess sludge hydrolysis and decomposition with different lysozyme dosage［J］. Journal of Hazardous Materials，2019，366：395-401. doi:10.1016/j.jhazmat.2018.12.002
11	CAO Xiuqin， LI Songyue， LIU Chaolei. Effect of lysozyme combined with hydrothermal pretreatment on excess sludge and anaerobic digestion［EB/OL］. （2023-10）［2023-11-15］.
12	HU Yingli， DAI Lingmei， LIU Dehua，et al. Progress & prospect of metal-organic frameworks（MOFs） for enzyme immobilization（enzyme/MOFs）［J］. Renewable and Sustainable Energy Reviews，2018，91：793-801. doi:10.1016/j.rser.2018.04.103
13	DONG Tianyu， ZHOU Xiaonan， DAI Yajie，et al. Application of magnetic immobilized enzyme of nano dialdehyde starch in deacidification of rice bran oil［J］. Enzyme and Microbial Technology，2022，161：110116. doi:10.1016/j.enzmictec.2022.110116
14	NOURI M， KHODAIYAN F. Green synthesis of chitosan magnetic nanoparticles and their application with poly-aldehyde kefiran cross-linker to immobilize pectinase enzyme［J］. Biocatalysis and Agricultural Biotechnology，2020，29：101681. doi:10.1016/j.bcab.2020.101681
15	LI Hao， KOU Beibei， YUAN Yali，et al. Porous Fe₃O₄@COF-immobilized gold nanoparticles with excellent catalytic performance for sensitive electrochemical detection of ATP［J］. Biosensors & Bioelectronics，2022，197：113758. doi:10.1016/j.bios.2021.113758
16	LIU Dongmei， DONG Chen. Recent advances in nano-carrier immobilized enzymes and their applications［J］. Process Biochemistry，2020，92：464-475. doi:10.1016/j.procbio.2020.02.005
17	张泽钰，李茹莹. 固定化微生物对河水的脱氮效果研究［J］. 环境科学学报，2020，40（1）：161-165. doi:10.13671/j.hjkxxb.2019.0360
	ZHANG Zeyu， LI Ruying. Study on the nitrogen removal in river water by immobilized microorganisms［J］. Acta Scientiae Circumstantiae，2020，40（1）：161-165. doi:10.13671/j.hjkxxb.2019.0360
18	YANG Qinxue， YAN Ying， YANG Xiaofang，et al. Enzyme immobilization in cage-like 3D-network PVA-H and GO modified PVA-H（GO@PVA-H） with stable conformation and high activity［J］. Chemical Engineering Journal，2019，372：946-955. doi:10.1016/j.cej.2019.04.216
19	WANG Ming， DONG Wenjing， GUO Yaoli，et al. Positively charged nanofiltration membranes mediated by a facile polyethyleneimine-Noria interlayer deposition strategy［J］. Desalination，2021，513：114836. doi:10.1016/j.desal.2020.114836
20	ZHANG Xinyu， LIN Zhenglun， WU Daoji，et al. Covalent codeposition modification of reverse osmosis membranes with Noria and zwitterionic copolymers for antifouling in reclaimed water production［J］. Desalination，2023，567：116973. doi:10.1016/j.desal.2023.116973
21	KUDO H， HAYASHI R， MITANI K，et al. Molecular waterwheel（noria） from a simple condensation of resorcinol and an alkanedial［J］. Angewandte Chemie（International Ed. in English），2006，45（47）：7948-7952. doi:10.1002/anie.200603013
22	赵瑜涵，王昊龙，周立山，等. 超声联合生物酶强化剩余污泥厌氧产酸性能研究［J］. 工业水处理，2023，43（11）：181-188. doi:10.19965/j.cnki.iwt.2022-1164
	ZHAO Yuhan， WANG Haolong， ZHOU Lishan，et al. Anaerobic acid production characteristics of excess sludge enhanced by ultrasound and biological enzymes［J］. Industrial Water Treatment，2023，43（11）：181-188. doi:10.19965/j.cnki.iwt.2022-1164
23	ZHAI Zhe， JIANG Chi， ZHAO Na，et al. Fabrication of advanced nanofiltration membranes with nanostrand hybrid morphology mediated by ultrafast Noria-polyethyleneimine codeposition［J］. Journal of Materials Chemistry A，2018，6（42）：21207-21215. doi:10.1039/c8ta08273a
24	TIAN Ye， CAO Yewen， WANG Yu，et al. Realizing ultrahigh modulus and high strength of macroscopic graphene oxide papers through crosslinking of mussel-inspired polymers［J］. Advanced Materials，2013，25（21）：2980-2983. doi:10.1002/adma.201300118
25	WAN Juanjuan， ZHANG Lijuan， JIA Boyu，et al. Effects of enzymes on organic matter conversion in anaerobic fermentation of sludge to produce volatile fatty acids［J］. Bioresource Technology，2022，366：128227. doi:10.1016/j.biortech.2022.128227
26	WANG Tianfeng， SHAO Liming， LI Tianshui，et al. Digestion and dewatering characteristics of waste activated sludge treated by an anaerobic biofilm system［J］. Bioresource Technology，2014，153：131-136. doi:10.1016/j.biortech.2013.11.066
27	李潜. 溶菌酶高产菌株的筛选及其产酶研究［D］. 保定：河北农业大学，2008.
	LI Qian. Screening for high lysozyme-yielding strain and the studies on fermentation of producing lysozyme ［D］. Baoding：Hebei Agricultural University，2008.
28	XIN Xiaodong， HE Junguo， LI Lin，et al. Enzymes catalyzing pre-hydrolysis facilitated the anaerobic fermentation of waste activated sludge with acidogenic and microbiological perspectives［J］. Bioresource Technology，2018，250：69-78. doi:10.1016/j.biortech.2017.09.211
29	CHEN Yinguang， JIANG Su， YUAN Hongying，et al. Hydrolysis and acidification of waste activated sludge at different pHs［J］. Water Research，2007，41（3）：683-689. doi:10.1016/j.watres.2006.07.030
30	HE Zhangwei， LIU Wenzong， TANG Congcong，et al. Performance and microbial community responses of anaerobic digestion of waste activated sludge to residual benzalkonium chlorides［J］. Energy Conversion and Management，2019，202：112211. doi:10.1016/j.enconman.2019.112211
31	DAREIOTI M A， KORNAROS M. Effect of hydraulic retention time（HRT） on the anaerobic co-digestion of agro-industrial wastes in a two-stage CSTR system［J］. Bioresource Technology，2014，167：407-415. doi:10.1016/j.biortech.2014.06.045
32	WANG Qingyan， ZHANG Panyue， BAO Shuai，et al. Chain elongation performances with anaerobic fermentation liquid from sewage sludge with high total solid as electron acceptor［J］. Bioresource Technology，2020，306：123188. doi:10.1016/j.biortech.2020.123188
33	LIU Xiang， WU Fengjie， ZHANG Min，et al. Role of potassium ferrate in anaerobic digestion of waste activated sludge：Phenotypes and genotypes［J］. Bioresource Technology，2023，383：129247. doi:10.1016/j.biortech.2023.129247
34	XI Shihao， DONG Xinlei， LIN Qingshan，et al. Enhancing anaerobic fermentation of waste activated sludge by investigating multiple electrochemical pretreatment conditions：Performance，modeling and microbial dynamics［J］. Bioresource Technology，2023，368：128364. doi:10.1016/j.biortech.2022.128364
35	WANG Zhihui， FANG Qian， LUO Jin，et al. Optimized process for the odd-carbon volatile fatty acids（OCFA） directional production：Anaerobic co-digestion of disused grease with sludge by anaerobic sequencing batch reactor［J］. Journal of Water Process Engineering，2022，46：102592. doi:10.1016/j.jwpe.2022.102592

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