摘要：

对菌种Trametes versicolor SYBC-L3发酵产漆酶的碳氮源进行了优化， 研究了菌株L3漆酶的性质及其在毛纺废水脱色中的作用。结果表明，其最佳发酵碳氮源为：麦芽糖12 g/L、豆粕4 g/L，优化后的漆酶的酶活在第8天可高达48 400 U/L；酶最适反应温度为50 ℃，在70 ℃以下保温1 h，仍然有70%的剩余活力，其最适pH为3.5，在pH4～8下保温24 h，仍然有70%以上的活力。用L3漆酶处理毛纺废水，20 min脱色率达到70%，有较好的脱色效果。

关键词: 白腐真菌, 漆酶, 脱色

Abstract:

Optimization of medium components for the production of laccase by the strain Trametes versicolor.SYBC-L3 has been studied and the effect of the laccase on woolen textile decolorization has been detected with the collected wastewater from a woolen textile factory. Its optimal carbon and nitrogen sources are as follows： maltose 12g/L, soybean meal 4g/L. The optimized laccase activity reaches 48 400 U/L on the 8th day which was significantly higher than that was previously reported. The characteristics of laccase are also investigated. It shows that the most suitable reaction temperature is 50 ℃. When the temperature keeps at 70 ℃ for l h, the remained activity is still 70%. Its most suitable pH is 3.5. When pH is 4-8, and the temperature keeps for 24 h, the remained activity is still 70%. When the woolen textile wastewater is treated with laccase L3 for 20 min, 70% of decolorization rate has been achieved, indicating the decolorization capacity of laccase is quite good.

Key words: white-rot fungus, laccase, decolorization

中图分类号: 

• Q939.9

• X791