还原蓝4高效降解菌的分离鉴定及降解条件优化

doi:10.19965/j.cnki.iwt.2021-0071

摘要/Abstract

摘要：

从印染废水活性污泥中筛分出一株还原蓝4（VB4）染料降解菌株，并对其降解条件进行优化。通过微观形态、生理生化和16S rRNA鉴定种属，利用单因素实验初步确定了VB4降解的影响因子，进一步应用PB实验、最陡爬坡实验、CCD实验及响应面法对其降解条件进行了优化及预测。结果表明：该菌株为Pseudomonas aeruginosa属细菌；最佳降解条件：pH=7.35，尿素质量浓度0.95 g/L，温度35.37℃，该条件下VB4脱色率可达97%，与预测值97.24%相差0.24%。建立的模型对处理VB4染料废水的预测效果较好，也为后续印染废水的处理提供了理论基础。

关键词: 印染废水, 还原蓝4, 脱色菌, 响应面优化

Abstract:

A strain of vat blue 4(VB4) dye decolorization was isolated from the printing and dyeing activated sludge, and its degradation conditions for VB4 was optimized. Species were identified by microscopic morphology, physiologicalbiochemical properties and its 16S rRNA gene sequence analysis. Single-factor experiment was used to preliminarily determine the factors affecting VB4 degradation, and Placket-Burman experiment, steepest ascent experiment, Central Composite Design experiment and response surface analysis were used to optimize and predict the degradation conditions. The results showed that the strain was identified as Pseudomonas aruginosa, the optimal degradation conditions on VB4 were: pH 7.35, urea concentration 0.95 g/L, the temperature 35.37℃, the degradation rate reached 97%, which was 0.24% difference from the predicted value of 97.24%. The established model predicted well for treatment of VB4 dye wastewater. All the results provided some information for the effective treatment of the printing and dyeing wastewater.

Key words: printing and dyeing wastewater, vat blue 4, decolorization bacteria, response surface analysis

中图分类号:

X703

邓田田,李海红,王洋涛. 还原蓝4高效降解菌的分离鉴定及降解条件优化[J]. 工业水处理, 2021, 41(10): 83-90.

Tiantian DENG,Haihong LI,Yangtao WANG. Isolation and identification of high-efficiency vat blue 4 dye-degrading strain and its degradation condition optimization[J]. Industrial Water Treatment, 2021, 41(10): 83-90.

https://www.iwt.cn/CN/Y2021/V41/I10/83

图/表 16

参考文献 33

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因素水平	A温度/℃	B初始接种量（OD₆₀₀）	CpH	D葡萄糖/（g·L^-1）	E尿素/（g·L^-1）	F染料/（mg·L^-1）	G微量盐溶液（mL·L^-1）
高水平	40	0.08	8	1.5	1.5	80	4
低水平	30	0.04	6	0.5	0.5	20	2
中心	35	0.06	7	1	1	50	3

因素水平	A温度/℃	B初始接种量（OD₆₀₀）	CpH	D葡萄糖/（g·L^-1）	E尿素/（g·L^-1）	F染料/（mg·L^-1）	G微量盐溶液（mL·L^-1）
高水平	40	0.08	8	1.5	1.5	80	4
低水平	30	0.04	6	0.5	0.5	20	2
中心	35	0.06	7	1	1	50	3

自变量	水平
自变量	最低	低	中心	高	最高
分辨率	-1.68	-1	0	1	1.68
A-温度/℃	26.6	30	35	40	43.4
C-pH	5.32	6	7	8	8.68
E-尿素/（g·L^-1）	0.4	0.6	0.9	1.2	1.4

自变量	水平
自变量	最低	低	中心	高	最高
分辨率	-1.68	-1	0	1	1.68
A-温度/℃	26.6	30	35	40	43.4
C-pH	5.32	6	7	8	8.68
E-尿素/（g·L^-1）	0.4	0.6	0.9	1.2	1.4

实验序号	温度/℃	初始接种量（OD₆₀₀）	pH	葡萄糖/（g·L^-1）	尿素/（g·L^-1）	染料/（mg·L^-1）	微量盐溶液/（mL·L^-1）	脱色率/%
1	40	0.08	6	1.5	1.5	20	2	53.43
2	40	0.08	8	1.5	0.5	80	2	81.88
3	40	0.04	8	0.5	1.5	80	4	44.38
4	40	0.04	8	0.5	1.5	20	2	68.49
5	30	0.04	8	1.5	0.5	80	2	80.62
6	30	0.08	8	0.5	0.5	20	4	90.41
7	40	0.04	6	1.5	0.5	20	4	56.16
8	30	0.04	6	0.5	0.5	20	2	54.79
9	30	0.08	8	1.5	1.5	20	4	68.75
10	40	0.08	6	0.5	0.5	80	4	40.88
11	30	0.08	6	0.5	1.5	80	2	35.75
12	30	0.04	8	1.5	1.5	80	4	38.88

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