工业水处理 ›› 2018, Vol. 38 ›› Issue (11): 21-26. doi: 10.11894/1005-829x.2018.38(11).021

• 试验研究 • 上一篇    下一篇

1株肠杆菌脱氮性能及其同步硝化反硝化机制初探

林而舒1, 陶欣1, 胡开辉1,2, 杨云龙1,2   

  1. 1. 福建农林大学生命科学学院, 福建福州 350000;
    2. 福建农林大学(古田)菌业研究院, 福建古田 352000
  • 收稿日期:2018-09-11 出版日期:2018-11-20 发布日期:2018-11-16
  • 作者简介:林而舒(1993-),硕士。E-mail:yylles@126.com。
  • 基金资助:
    国家自然科学基金(21407024);福建省自然科学基金(2015J1594)

First exploration on the denitrification capability of a strain of enterobacterium and its simultaneous nitrification and denitrification mechanisms

Lin Ershu1, Tao Xin1, Hu Kaihui1,2, Yang Yunlong1,2   

  1. 1. School of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350000, China;
    2. Institute of Mushroom Industry, Fujian Agriculture and Forestry University(Gutian), Gutian 352000, China
  • Received:2018-09-11 Online:2018-11-20 Published:2018-11-16

摘要: 从序批式反应器活性污泥中分离出1株异养硝化-好氧反硝化细菌——Enterobacter asburiae YT。利用单因素实验得出最佳脱氮条件:柠檬酸钠为碳源,温度30℃,C/N=12,溶氧为0.9 mg/L。通过对该菌株同步硝化反硝化的氮平衡分析,发现24 h内氨氮的去除率达到100%,其中同化到细胞中的氮为30.5%,而以气态氮形式产生的氮损失达到58.38%。菌株YT是一种新型且具有高效脱氮能力的微生物,在生物脱氮领域具有较好的应用价值。

关键词: Enterobacter asburiae, 生物脱氮, 同步硝化反硝化

Abstract: A strain of heterotrophic nitrification-aerobic denitrification bacterium has been isolated from the activated sludge in a sequencing batch reactor. The strain is initially identified as Enterobacter asburiae YT. The optimal nitrogen removal conditions are obtained by means of single factor experiments as follows:using sodium citrate as carbon source,temperature is 30℃,C/N=12 and dissolved oxygen 0.9 mg/L. Based on the nitrogen balance analysis of simultaneous nitrification and denitrification of the strain,it is found that the ammonia nitrogen removing rate reaches 100% within 24 h,of which the percentage of nitrogen assimilated into the cells is 30.5%,and the percentage of nitrogen loss in gaseous state nitrogen is 58.38%. It shows that strain YT is a new type of microbes having highly efficient denitrification ability,which has good application values in the field of biological denirification.

Key words: Enterobacter asburiae, biological denitrification, simultaneous nitrification and denitrification

中图分类号: