EBPR系统中聚糖菌及其反硝化代谢机理的研究进展

doi:10.11894/iwt.2018-0857

摘要/Abstract

摘要： 聚糖菌（GAOs）作为强化生物除磷（EBPR）系统中重要的微生物组成部分，具有反硝化脱氮的作用，但目前国内外关于GAOs的富集培养及反硝化代谢机理的研究尚不完善。综述了GAOs的种群类型以及反硝化聚糖菌（DGAOs）的代谢机理，着重归纳了碳源、pH、温度等因素对GAOs富集生长的影响，并在此基础上，对GAOs的研究现状及其研究方向进行了总结和展望，以期对GAOs的生理性能及强化生物除磷系统的稳定运行提供理论参考。

关键词: 强化生物除磷, 聚糖菌, 反硝化聚糖菌, 代谢机理, 生长富集

Abstract: As important microbial components in the enhanced biological phosphorus removal(EBPR) system,glycogen accumulating organisms(GAOs) have the functions of denitrification. However,at present,there are limited studies on the enrichment culture and denitrification mechanism of GAOs. The population types of GAOs and the metabolic mechanism of denitrifying glycogen accumulating organisms(DGAOs) were reviewed. The effects of carbon source,pH value and temperature on the enrichment and growth of GAOs were summarized. On the basis of this summary,the research status and future direction of GAOs were discussed as well. This study provides a theoretical reference for improving the physiological performance of GAOs and maintaining the stable operation of the EBPR system.

Key words: enhanced biological phosphorus removal (EBPR), glycogen accumulating organisms (GAOs), denitrifying glycogen accumulating organisms (DGAOs), metabolic mechanism, growth enrichment

中图分类号:

X703

常烁, 曾薇. EBPR系统中聚糖菌及其反硝化代谢机理的研究进展[J]. 工业水处理, 2019, 39(9): 8-13.

Chang Shuo, Zeng Wei. Glycogen accumulating organisms and its denitrifying metabolic mechanism in enhanced biological phosphorus removal systems: a review[J]. Industrial Water Treatment, 2019, 39(9): 8-13.

https://www.iwt.cn/CN/Y2019/V39/I9/8

参考文献

[1] Tayà C,Garlapati V K,Guisasola A,et al. The selective role of nitrite in the PAO/GAO competition[J]. Chemosphere,2013,93(4):612-618.
[2] Whang L M,Park J K. Competition between polyphosphateand glycogen-accumulating organisms in enhanced-biological-phosphorusremoval systems:effect of temperature and sludge age[J]. Water Environment Research,2006,78(1):4-11.
[3] Crocetti G R,Bond P L,Banfield J F,et al. Glycogen-accumulating organisms in laboratory-scale and full-scale wastewater treatment processes B[J]. Microbiology,2002,148(11):3353-3364.
[4] Wong M T. Identification and occurrence of tetrad-forming Alphaproteobacteria in anaerobic-aerobic activated sludge processes[J]. Microbiology,2004,150(11):3741-3748.
[5] Meyer R L. Putative glycogen-accumulating organisms belonging to the Alphaproteobacteria identified through rRNA-based stable isoto-pe probing[J]. Microbiology,2006,152(2):419-429.
[6] Burow L C,Kong Y,Nielsen J L,et al. Abundance and ecophysiology of Defluviicoccus spp.,glycogen-accumulating organisms in fullscale wastewater treatment processes[J]. Microbiology,2007,153(1):178-185.
[7] Wong M T,Liu W T. Ecophysiology of Defluviicoccus-related tetrad-forming organisms in an anaerobic-aerobic activated sludge process[J]. Environmental Microbiology,2007,9(6):1485-1496.
[8] Nittami T,McIlroy S,Seviour E M,et al. Candidatus Monilibacter spp.,common bulking filaments in activated sludge,are members of Cluster Ⅲ Defluviicoccus[J]. Systematic and Applied Microbiology, 2009,32(7):480-489.
[9] McIlroy S J,Seviour R J. Elucidating further phylogenetic diversity among the Defluviicoccus-related glycogen-accumulating organisms in activated sludge[J]. Environmental Microbiology Reports,2009, 1(6):563-568.
[10] Stokholm-Bjerregaard M,McIlroy S J,Nierychlo M,et al. A critical assessment of the microorganisms proposed to be important to enhanced biological phosphorus removal in full-scale wastewater treatment systems[J]. Frontiers in Microbiology,2017,8:718.
[11] McIlroy S J,Onetto C A,McIlroy B,et al. Genomic and in situ analyses reveal the Micropruina spp. as abundant fermentative glycogen accumulating organisms in enhanced biological phosphorus removal systems[J]. Frontiers in Microbiology,2018,9:1004.
[12] Kong Y H,Xia Y,Nielsen J L,et al. Ecophysiology of a group of uncultured Gammaproteobacterial glycogen-accumulating organisms in full-scale enhanced biological phosphorus removal wastewater treatment plants[J]. Environmental Microbiology,2006,8(3):479-489.
[13] Zhao W H,Huang Y,Wang M X,et al. Post-endogenous denitrification and phosphorus removal in an alternating anaerobic/oxic/anoxic(AOA) system treating low carbon/nitrogen(C/N) domestic wastewater[J]. Chemical Engineering Journal,2018,339:450-458.
[14] Miao L,Wang S Y,Li B K,et al. Advanced nitrogen removal via nitrite using stored polymers in a modified sequencing batch reactor treating landfill leachate[J]. Bioresource Technology,2015,192:354-360.
[15] 贾淑媛,王淑莹,赵骥,等. 驯化后的聚糖菌对NO₂^-N和NO₃^--N内源反硝化速率的影响[J]. 化工学报,2017,68(12):4731-4738.
[16] Zhu X Y,Chen Y G. Reduction of N₂O and NO generation in Anaerobic-Aerobic(low dissolved oxygen) biological wastewater treatment process by using sludge alkaline fermentation liquid[J]. Environmental Science & Technology,2011,45(6):2137-2143.
[17] Ribera-Guardia A,Marques R,Arangio C,et al. Distinctive denitrifying capabilities lead to differences in N₂O production by denitrifying polyphosphate accumulating organisms and denitrifying glycogen accumulating organisms[J]. Bioresource Technology,2016, 219:106-113.
[18] Kim J M,Lee H J,Lee D S,et al. Identification of a novel subgroup of uncultured gammaproteobacterial glycogen-accumulating organisms in enhanced biological phosphorus removal sludge[J]. Microbiology,2011,157(8):2287-2296.
[19] Shen N,Chen Y,Zhou Y. Multi-cycle operation of enhanced biological phosphorus removal(EBPR) with different carbon sources under high temperature[J]. Water Research,2017,114:308-315.
[20] Burow L C,Mabbett A N,McEwan A G,et al. Bioenergetic models for acetate and phosphate transport in bacteria important in enhanced biological phosphorus removal[J]. Environmental Microbiology, 2010,10(1):87-98.
[21] Lü X M,Shao M F,Li C L,et al. Bacterial diversity and community structure of denitrifying phosphorus removal sludge in strict anaerobic/anoxic systems operated with different carbon sources[J]. Journal of Chemical Technology & Biotechnology,2014,89(12):1842-1849.
[22] Oehmen A,Yuan Z G,Blackall L L,et al. Comparison of acetate and propionate uptake by polyphosphate accumulating organisms and glycogen accumulating organisms[J]. Biotechnology and Bioengineering,2005,91(2):162-168.
[23] Guo G,Wu D,Ekama G A,et al. Denitrifying sulfur conversionassociated EBPR:Effects of temperature and carbon source on anaerobic metabolism and performance[J]. Water Research,2018, 141:9-18.
[24] Wang N D,Peng J,Hill G. Biochemical model of glucose induced enhanced biological phosphorus removal under anaerobic condition[J]. Water Research,2002,36(1):49-58.
[25] Bond P L,Keller J,Blackall L L. Anaerobic phosphate release from activated sludge with enhanced biological phosphorus removal. A possible mechanism of intracellular pH control[J]. Biotechnology and Bioengineering,1999,63(5):507-515.
[26] Guo G,Wu D,Hao T W,et al. Denitrifying sulfur conversion-associated EBPR:The effect of pH on anaerobic metabolism and performance[J]. Water Research,2017,123:687-695.
[27] Filipe C D M,Daigger G T,Grady C P L. A metabolic model for acetate uptake under anaerobic conditions by glycogen accumulating organisms:Stoichiometry,Kinetics,and the effect of pH[J]. Biotechnology and Bioengineering,2001,76(1):17-31.
[28] Filipe C D M,Daigger G T,Grady C P L. Effects of pH on the rates of aerobic metabolism of phosphate-accumulating and glycogen-accu mulating organisms[J]. Water Environment Research,2001,73(2):213-222.
[29] Lopez-Vazquez C M,Song Y I,Hooijmans C M,et al. Short-term temperature effects on the anaerobic metabolism of glycogen accumulating organisms[J]. Biotechnology and Bioengineering,2007,97(3):483-495.
[30] Lopez-Vazquez C M,Song Y I,Hooijmans C M,et al. Temperature effects on the aerobic metabolism of glycogen-accumulating organisms[J]. Biotechnology and Bioengineering,2008,101(2):295-306.
[31] Li Y Y,Cope H A,Rahman S M,et al. Toward better understanding of EBPR systems via linking raman-based phenotypic profiling with phylogenetic diversity[J]. Environmental Science & Technology, 2018,52(15):8596-8606.
[32] Carvalheira M,Oehmen A,Carvalho G,et al. The impact of aeration on the competition between polyphosphate accumulating organisms and glycogen accumulating organisms[J]. Water Research,2014, 66(15):296-307.

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