金霉素对强化生物除磷系统除磷性能的影响及机制

doi:10.19965/j.cnki.iwt.2023-1115

摘要/Abstract

摘要：

为阐明金霉素（CTC）对强化生物除磷（EBPR）系统除磷性能的影响及机制，采用序批式反应器，以酪蛋白水解物为唯一碳源，研究了CTC对EBPR系统的影响。结果表明，当CTC投加量为0.1 mg/L时，PO₄ ^3-和COD的去除率与对照组相似；当CTC投加量为1.0 mg/L时，PO₄ ^3-去除率从90.0%下降为75.0%，COD去除率从95.3%下降为90.4%；当CTC投加量为5.0 mg/L时，PO₄ ^3-去除率仅为40.0%，遭到显著抑制，但COD的去除率仍然达到85.1%，随着CTC投加量的增加，其对生物除磷系统的抑制作用加强。典型周期内基质代谢过程分析发现，CTC对生物除磷中的好氧吸磷过程影响更为显著，会降低糖原和多聚磷酸盐的合成。宏基因组学分析表明CTC的存在会抑制磷去除的相关代谢途径和聚羟基烷酸盐（PHB）合成过程中部分关键酶的合成，进而导致系统除磷性能恶化。

关键词: 强化生物除磷, 金霉素, 聚磷菌, 磷代谢途径, 宏基因组学

Abstract:

A sequencing batch reactor(SBR) was used with casein hydrolysate as the sole carbon source to investigate the impact of chlortetracycline(CTC) on the enhanced biological phosphorus removal(EBPR) system. The results indicated that the removal rates of PO₄³⁻ and COD were comparable to those of the control group when the CTC concentration was 0.1 mg/L. However, the removal rate of PO₄³⁻ decreased from 90.0% to 75.0%, and the removal rate of COD decreased from 95.3% to 90.4% when the CTC concentration was increased to 1.0 mg/L. Further, at CTC concentration of 5.0 mg/L, the removal rate of PO₄³⁻ was only 40.0%, indicating significant inhibition, while the removal rate of COD still reached 85.1%. The inhibitory effect of chlortetracycline on the biological phosphorus removal system was intensified with the increase of chlortetracycline dosage. Analysis of matrix metabolism in typical cycles revealed that chlortetracycline had a more pronounced effect on aerobic phosphorus uptake in biological phosphorus removal, which would reduce glycogen and polyphosphate synthesis. Metagenomic analysis demonstrated that the presence of chlortetracycline could inhibit the metabolic pathways associated with phosphorus removal and the synthesis of key enzymes in poly-β-hydroxybutyrate(PHB) synthesis. This inhibition ultimately resulted in the deterioration of the phosphorus removal performance of the system.

Key words: enhanced biological phosphorus removal, chlortetracycline, phosphate accumulating bacteria, phosphorus metabolic pathway, metagenomics

中图分类号:

X703.1

鄢雨萌, 任晨竹, 王少坡, 刘灵婕, 孟凡盛, 邱春生, 于静洁. 金霉素对强化生物除磷系统除磷性能的影响及机制[J]. 工业水处理, 2024, 44(12): 50-58.

Yumeng YAN, Chenzhu REN, Shaopo WANG, Lingjie LIU, Fansheng MENG, Chunsheng QIU, Jingjie YU. Effect and mechanism of chlortetracycline on phosphorus removal performance in EBPR system[J]. Industrial Water Treatment, 2024, 44(12): 50-58.

https://www.iwt.cn/CN/Y2024/V44/I12/50

图/表 10

图1 反应器装置

Fig.1 Schematic diagram of reactor

图2 系统PO₄ ^3-和COD去除率变化情况

Fig.2 PO₄ ^3- and COD concentrations in the system

图3 系统典型周期PO₄ ^3-（a）、COD（b）、Ploy-P（c）和Gly（d）浓度变化

Fig.3 PO₄ ^3-（a）， COD（b）， Ploy-P（c）， and Gly（d） concentrations during typical periods of the system

图4 系统中EPS的各组分变化

Fig.4 Changes in EPS components in the system

图5 系统污泥性能变化

Fig.5 Changes in sludge performance of the system

图6 微生物门水平相对丰度变化

Fig.6 Relative abundance at the phylum level

图7 微生物属水平相对丰度变化

Fig.7 Relative abundance at the genus level

图8 磷代谢相关途径

Fig.8 Pathways related to phosphorus metabolism

图9 PHA代谢、糖原代谢关键酶的基因丰度

Fig.9 Gene abundance of key enzymes in PHA metabolism，glycogen metabolism

图10 磷代谢关键酶的基因丰度

Fig.10 Gene abundance of key enzymes in phosphorus metabolism

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