摘要：

通过实验建立了工业废水中盐酸苯肼的高效液相色谱分析方法。色谱条件为:Kromasil-C₁₈(150 mm×4.6 mm,5μm)色谱柱,流速1 mL/min,柱温30℃,进样体积10μL,流动相为V(甲醇):V(8 mmol/L KH₂PO₄+4 mmol/LK₂HPO₄)=35:65,测定波长235 nm。测定结果表明,线性范围为0.1～100 mg/L,平均加标回收率为98.2%,相对标准偏差为1.06%。该方法操作简单,分析快速、准确。

关键词: 盐酸苯肼, 高效液相色谱法, 工业废水

Abstract:

The HPLC method for determining phenylhydrazine hydrochloride in industrial wastewater has been established.The chromatogram conditions are as follows:Kromasil-C₁₈ column(150 mm×4.6 mm,5 μm),velocity of flow 1 mL/min,column temperature 30 ℃,sample volume 10 μL,mobile phase is V(methanol):V(8 mmol/L KH₂PO₄+4 mmol/L K₂HPO₄)=35:65,and wavelength 235 nm.The results show that the method offeres good linearity from 0.1-100 mg/L,average recovery rate is 98.2% and RSD 1.06%.This method is characterized by easy operation,and rapid and accurate analysis.

Key words: phenylhydrazine hydrochloride, high performance liquid chromatography, industrial wastewater

中图分类号: 

• X832