Inducement of laccase production by amanita verna and its use for the decolorization of azodye

doi:10.11894/1005-829x.2008.28(9).70

Abstract

Abstract:

Amanita verna is an excellent producer of the industrially important enzyme laccase. The secretion and degradation of direct black dye under various nutritious conditions are discussed. When wheat bran and yeast extract are used as carbon source and nitrogen source respectively, and cultivated at 120r/min, a higher yield level is obtained. In addition, when the concentration of the dye is lower(10 mg/L), the effect of laccase on the degradation speed of dye is rapid and thorough. When the pH is 4.5 and temperature 50 ℃, azo dye direct black G can be degraded effectively by crude laccase(10U/L). And at 120r/min, the degradation rate reaches 84.4%.

Key words: amanita verna, azo dye, decolorization, laccase

摘要：

白毒鹅膏菌是一种重要的产生胞外漆酶的菌株。探讨了漆酶在各种营养条件下的分泌情况以及对直接黑染料的降解情况。结果表明，以麸皮作为碳源，酵母膏作为氮源，在120r/min的转速下培养，漆酶的产量最高。另外染料在较低质量浓度(10mg/L)时，漆酶对染料的降解迅速而彻底，在pH=4.5、50℃条件下，偶氮染料直接黑G能被粗酶液(10U/L)有效地降解，在120r/min时降解率达到84.4%。

关键词: 白毒鹅膏菌, 偶氮染料, 脱色, 漆酶

CLC Number:

Dong Xuewei, Zhu Qizhong, Yu Xiumin, Xu Guoying, Lü Xinping, Yu Tao, Wang Fangzhong. Inducement of laccase production by amanita verna and its use for the decolorization of azodye[J]. INDUSTRIAL WATER TREATMENT, 2008, 28(9): 70-73.

董学卫, 朱启忠, 于秀敏, 徐国英, 吕新萍, 于涛, 王方忠. 白毒鹅膏菌漆酶的诱导及其对直接黑G的脱色作用[J]. 工业水处理, 2008, 28(9): 70-73.

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References

[1] Solomon E I, Sundaram U M, Machonkin T E. Multicopper Oxidases and Oxygenases[J]. Chem. Rev., 1996, 96(7) : 2563-2605.
[2] Pointing S B. Feasibility of bioremediation by white-rot fungi [J].Appl. Microbiol. Biotechnol., 2001, 57(1-2) :20-33.
[3] Moreira M T, Mielgo I, Feijoo G, et al. Evaluation of different fungal strains in the decolourisation of synthetic dyes [J].Biotechnol. Lett., 2000, 22(18) :1499-1503.
[4] Rai H S, Bhattacharyya M S, Singh J, et al.Removal of dyes from the effluent of textile and dyestuff manufacturing industry: a review of emerging techniques with reference to biological treatment [J].Crit. Rev. Environ. Sci. Technol., 2005, 35(3) :219-238.
[5] Moreira M T, Palma C, Mielgo I, et al. In vitro degradation of a polymeric dye (Poly R -478) by manganese peroxidase [J].Biotechnol. Bioeng., 2001, 75(3) :362-368.
[6] Zouari -Mechichi H, Mechichi T, Dhouib A, et al. Laccase purification and characterization from Trametes trogii isolated in Tunisia: decolorization of textile dyes by the purified enzyme [J].Enzyme Microb. Technol., 2006, 39(1) :141-148.
[7] Nyanhongo G S, Gomes J, Gubitz G M, et al. Decolorization of textile dyes by laccases from a newly isolated strain of Trametes modesta[J]. Water Research, 2002, 36(6) :1449-1456.
[8] Conneely A, Smyth W F, McMullan G. Study of the white-rot fungal degradation of selected phthalocyanine dyes by capilary electrophoresis and liquid chromatography [J]. Anal. Chim. Acta,2002, 451(2) :259-270.
[9] Jarosz-Wilkolazka A, Kochmanska-Rdest J, Malarczyk E,et al.Fungi and their ability to decolourize azo and anthraquinonic dyes[J]. Enzyme Microb. Technol., 2002, 30(4) :566-572.
[10] Martins M A M, Lima N. Comparative studies of fungal degradation of single or mixed bioaccessible reactive azo dyes[J].Chemosphere, 2003,52(6) :967-973.
[11] Moldes D, Couto S R, Cameselle C, et al. Study of the degradation of dyes by MnP of Phanerochaete chrysosporium produced in a fixed-bed bioreactor [J]. Chemosphere, 2003, 51(4) :295-303.
[12] Heinzkill M, Bech L, Halkier T, et al. Characterization of laccase, and peroxidases from wood-rotting fungi [J]. Appl.Environ. Microbiol., 1998, 64(5) :1601-1606.
[13] Galhaup C, Wagner H, Hinterstoisser B, et al. Increased production of laccase by the wood -degrading basidiomycete Trametes pubescens[J]. Enzyme Microb. Technol., 2002, 30(4) :529-536.
[14] Yang Q, Yang M, Pritsch K, et al. Decolorization of synthetic dyes and production of manganese-dependent peroxidase by new fungal isolates[J]. Biotechnol. Lett., 2003, 25(9) :709-713.

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