Abstract: Aspergillus fumigatus mycelial pellets have been used as biological immobilization carrier,and co-immobilized with another kind of laccase-producing Pseudomonas fluorescens to form composite mycelial pellets. The influences of incubation time,temperature,pH,speed,and inoculum concentration on laccase activity have been investigated. It is found that when the initial mass concentration of Cango Red is 100 mg/L,temperature 30℃,160 r/min, and pH=5,the dye decolorization rates by composite mycelialpellets and crude enzyme are 99.98% and 82.36%,respectively. It is also found through observation by means of scanning electron microscopy(SEM) that Pseudomonas fluorescens can successfully be immobilized on fungal mycelium pellets and have porous network structures,which is good for mass transfer and oxygen transfer. Therefore,the composite mycelial pellets has strong decolorization and degradation capabilities for dye solutions.

Key words: composite mycelial pellet, Congo Red, laccase, decolorization, degradation

摘要： 取烟曲霉菌丝球作为生物固定化载体，与另一株产漆酶的荧光假单胞杆菌共固定化形成混合菌丝球。考察了培养时间、温度、pH、转速、接种量对漆酶酶活性的影响。当刚果红染料初始质量浓度为100 mg/L、30℃、160r/min、pH为5时，混合菌丝球与粗酶液对染料的脱色率分别为99.98%、82.36%。扫描电镜观察发现，荧光假单胞杆菌成功固定在真菌菌丝球上并具有多孔网状结构，有利于传质传氧。因此，混合菌丝球对染料有脱色与降解能力。

关键词: 混合菌丝球, 刚果红, 漆酶, 脱色, 降解

CLC Number: 

• X703