Effect of HRT on operation performance of sulfur/siderite autotrophic denitrification system

doi:10.19965/j.cnki.iwt.2022-0117

Abstract

Abstract:

A combined sulfur/siderite system was constructed for autotrophic denitrification experiment. The effects of hydraulic retention time（HRT） on the synergistic denitrification efficiency，nitrogen intermediates，SO₄ ^2- concentration of effluent，autotrophic denitrification contribution proportion of FeCO₃ and changes of microbial community structure were explored. The results showed that when HRT was 12 h，the total inorganic nitrogen（TIN） removal rate of the system was 95.67%，the SO₄ ^2- mass concentration of effluent was 181.26 mg/L，and the proportion of FeCO₃ autotrophic denitrification increased to 20.49%. The accumulation of intermediate products and the formation of SO₄ ^2- were the least under this condition，which indicated that HRT=12 h was the suitable operating condition for the combined system. Thiobacillus，the most abundant autotrophic denitrifying bacteria in the system，utilized sulfur and Fe（Ⅱ） as electron donors to degrade NO₃ ^--N. Sulfur/siderite autotrophic denitrification technology had the characteristics of economical，efficient and synergistic denitrification，which would make it a promising new method for deep denitrification of tailwater of municipal wastewater treatment plants.

Key words: sulfur/siderite, autotrophic denitrification, denitrification, hydraulic retention time, community structure

摘要：

构建了硫/菱铁矿组合系统进行自养反硝化脱氮的实验，探究了水力停留时间（HRT）对系统的协同脱氮效率、氮的中间产物、出水SO₄ ^2-浓度、FeCO₃自养反硝化的脱氮贡献率及微生物群落结构变化的影响。研究结果表明，当HRT=12 h时，系统总无机氮（TIN）去除率为95.67%，出水SO₄ ^2-质量浓度为181.26 mg/L，并且FeCO₃自养反硝化脱氮对氮脱除的贡献率增加至20.49%，该HRT条件下积累的中间产物和生成的SO₄ ^2-最少，是硫/菱铁矿组合系统适宜的运行条件。硫杆菌属（Thiobacillus）是系统中丰度最高的自养反硝化细菌，其利用硫和Fe（Ⅱ）作为电子供体降解NO₃ ^--N。硫/菱铁矿自养反硝化脱氮技术具有经济高效及协同脱氮的特点，这将使其成为一种极具潜力的污水处理厂尾水深度脱氮的新方法。

关键词: 硫/菱铁矿, 自养反硝化, 脱氮, 水力停留时间, 群落结构

CLC Number:

X703.1

Xianxu ZHANG, Xiaoling HU, Li ZHANG, Hongyu WANG. Effect of HRT on operation performance of sulfur/siderite autotrophic denitrification system[J]. Industrial Water Treatment, 2022, 42(11): 177-183.

张献旭, 胡晓玲, 张俐, 王弘宇. HRT对硫/菱铁矿自养反硝化系统运行性能的影响[J]. 工业水处理, 2022, 42(11): 177-183.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2022-0117

https://www.iwt.cn/EN/Y2022/V42/I11/177

Figures/Tables 8

Fig. 1 Schematic diagram of reaction device

Fig. 2 Variations of nitrogen under different HRT conditions

Fig. 3 Variations of SO₄ ^2- and pH under different HRT conditions

Fig. 4 The proportions of FeCO₃ and sulfur autotrophic denitrification under different HRT conditions

Table 1 Species richness and diversity estimators of microbial populations under different HRT conditions

样品编号	OTU	Ace	Chao	Shannon	Simpson	Coverage
D6	192	330	287	1.48	0.503 0	0.998 1
D12	364	626	487	1.49	0.570 5	0.996 5
D18	171	351	266	0.63	0.818 7	0.997 8

Fig. 5 Venn diagram

Fig. 6 Distribution of microbial composition structure at phylum level

Fig. 7 Heatmap of microbial compositions at genus level

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