Analysis of Chlorella sp. growing and water purification capacity in the mixed wastewater coupled with microalgae

doi:10.19965/j.cnki.iwt.2021-0608

Abstract

Abstract:

The purification capability of four types of Chlorella to the wastewater as well as their influence mechanisms were investigated by utilizing a mixed wastewater coupling microalgae culture system. The high value-added products such as pigments and lipids produced in the process were examined. The results revealed that the selected four types of Chlorella had no obvious effect on nutrient removal in raw water of single soybean products or dairy products. However，in the mixed liquid coupling microalgae cultivate system，the purification effect was improved remarkably. When mixed volume ratio of single soybean products wastewater to dairy products wastewater was 1∶1，the Chlorella sp. had the strongest ability to remove TN and TP，and simultaneously exhibited good performance in removing COD as well. By this ratio，TN removal efficiency could reach 80.3%，TP removal efficiency was between 92.41%?95.48% and 6 555.5 mg/L of COD was removed. UTEX1602 had the best water purification capability and the high value products produced by it were more outstanding：the production of chlorophyll a reached 70.83 mg/L，the carbohydrate reaching 271.57 mg/L and polysaccharides up to 117 mg/L，the protein 6?7.5 times higher than other ratios and it exhibited the best lipids accumulation ability. This research can obtain additional products with higher utilization value and provide a scientific reference for the industrial wastewater purification.

Key words: mixed wastewater, coupling microalgae culture system, Chlorella sp., water purification, biomass, high value-added products

摘要：

利用混合废水耦合微藻培养系统，研究了4种小球藻的生长变化和废水净化能力及影响机制，并对其产生的色素、油脂等高附加值产物进行了测定。结果表明：4种小球藻对单一豆制品原水、奶牛场乳制品原水中的营养物质去除效果不明显；但在混合液耦合微藻系统中，其净化效果显著提高。当豆制品废水、奶牛场乳制品废水体积比为1∶1时，小球藻净化废水中TN、TP的能力最强，并有较高的COD净化能力，TN去除率达到80.3%，TP去除率达到92.41%~95.48%，COD最大去除量为6 555.5 mg/L；其中小球藻UTEX1602的水质净化能力最强，其产生的高附加值产物最多：叶绿素a达70.83 mg/L、碳水化合物达271.57 mg/L、多糖达117 mg/L、蛋白质超出其他比例混合废水的6~7.5倍、油脂富集能力也优于其他藻种。该研究可为高附加值产品生产及工业废水净化提供参考。

关键词: 混合废水, 耦合微藻培养, 小球藻, 水质净化, 生物量, 高附加值产品

CLC Number:

X703

Bing LI,Yan SHI,Di WU,Yan LI. Analysis of Chlorella sp. growing and water purification capacity in the mixed wastewater coupled with microalgae[J]. Industrial Water Treatment, 2022, 42(3): 114-122.

李冰,石岩,吴迪,李妍. 混合废水耦合微藻对小球藻生长及净水能力的研究[J]. 工业水处理, 2022, 42(3): 114-122.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2021-0608

https://www.iwt.cn/EN/Y2022/V42/I3/114

Figures/Tables 13

Table 1 The quality of two kinds of raw water and mixed wastewater

水质指标	豆制品废水	乳制品废水	1∶1混合废水	1∶5混合废水	1∶9混合废水
pH	4.18	10.89	7	7	7
TN/（mg·L^-1）	752.4	37.7	374.61	187.57	101.58
TP/（mg·L^-1）	85.5	3.11	40.71	16.74	8.71
COD/（mg·L^-1）	19 850	773.5	10 344	3 895	2 471.5
NH₄⁺-N/（mg·L^-1）	220.34	未测出	126.1	93.92	55.64

Fig. 1 The growth of Chlorella in raw water of soybean products

Fig. 2 The growth of Chlorella in raw water of dairy farm

Fig. 3 The growth of Chlorella in different mixing ratio wastewater

Fig. 4 Purification effect of four species of Chlorella on TN in mixing wastewater

Fig. 5 Purification effect of four species of Chlorella on NH₄⁺?N in mixing wastewater

Fig. 6 Purification effect of four species of Chlorella on TP in mixing wastewater

Fig. 7 Purification effect of four species of Chlorella on COD in mixing wastewater

Fig.8 Pigment production of four species of Chlorella in mixing wastewater

Fig. 9 Lipid production of four species of Chlorella in mixing wastewater

Fig. 10 Carbohydrate production of four species of Chlorella in mixing wastewater

Fig. 11 Protein production of four species of Chlorella in mixing wastewater

Fig. 12 Polysaccharides production of four species of Chlorella in mixing wastewater

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