摘要：

采用厌氧-好氧间歇运行模式，在SBR反应器中分别以丙酸钠、乙酸钠、葡萄糖、蔗糖为单一碳源对生物除磷颗粒污泥进行培养驯化，并考察不同碳源下除磷颗粒污泥对水中磷的去除效果，同时结合高通量测序，探究不同碳源驯化的生物除磷颗粒污泥中微生物种群结构的变化情况。结果证明：碳源为丙酸钠时，系统对磷的去除效果最佳。高通量测序结果表明：碳源对除磷颗粒污泥的微生物种群结构影响显著，以丙酸钠为碳源的颗粒污泥中聚磷菌（PAOs）占比最高；以乙酸钠为碳源的颗粒污泥聚糖菌（GAOs）占比最高；以蔗糖为碳源的颗粒污泥PAOs含量最低，对磷的去除效果最差。

关键词: 碳源, 除磷, 高通量测序

Abstract:

Anaerobic-aerobic intermittent operation mode was used to cultivate and domesticate biological phosphorus removal granular sludge in the SBR reactor, with sodium propionate, sodium acetate, glucose, and sucrose as the single carbon source, separately. The effect of granular sludge cultivated under different carbon sources on phosphorus removal was investigated. With high-throughput sequencing, the microbial population structure in these granular sludge was explored. Among them, the system with sodium propionate as carbon source had the best phosphorus removal effect. High-throughput sequencing results revealed that carbon sources had significant impact on the microbial population structure of phosphorus removal granular sludge. The granular sludge with sodium propionate as the carbon source had the highest proportion of phosphorus-aggregating bacteria (PAOs). The granular sludge with sodium acetate as the carbon source had the highest proportion of glycogen accumulating organisms (GAOs). While the granular sludge with sucrose as the carbon source had the lowest proportion of GAOs, with the worst phosphorus removal effect.

Key words: carbon source, phosphorus removal, high-throughput sequencing

中图分类号: 

• X703.1