Bioaugmentation to recover the impact caused by nonivamide in a sequencing batch reactor

doi:10.11894/iwt.2020-0073

Abstract

Abstract:

This paper explored the impact of nonivamide on sequencing batch reactor (SBR), and studied the effect and mechanism of complex bacteria to restore the impact caused by nonivamide. The results showed that under the effect of 100 mg/L nonivamide, the reactor performances significantly reduced, and the microbial community of activated sludge was impacted. The use of compound bacteria may degrade nonivamide directly and improved the living conditions of functional flora, recover community metabolic functions, and ultimately significantly improve the performances of SBR.

Key words: nonivamide, microbial community, bioaugmentation, functional flora

摘要：

探究了合成辣椒素对序批式反应器（SBR）的冲击情况，并研究了复合菌剂修复合成辣椒素冲击的效果和原理。结果表明在100 mg/L合成辣椒素的作用下，反应器的处理效率显著下降，活性污泥微生物群落受到冲击。复合菌剂的使用可能通过降解合成辣椒素，改善功能菌群的生存条件，恢复功能菌群的种类与含量，进而恢复了群落代谢功能，最终显著提高了SBR反应器的运行效果。

关键词: 合成辣椒素, 微生物群落, 生物强化, 功能菌群

CLC Number:

X703

Wenqian Xu,Jiangyu Ye. Bioaugmentation to recover the impact caused by nonivamide in a sequencing batch reactor[J]. Industrial Water Treatment, 2020, 40(11): 84-89.

徐文倩,叶姜瑜. 生物强化修复合成辣椒素对序批式反应器的影响[J]. 工业水处理, 2020, 40(11): 84-89.

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URL: https://www.iwt.cn/EN/10.11894/iwt.2020-0073

https://www.iwt.cn/EN/Y2020/V40/I11/84

Figures/Tables 6

References 15

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样品		Sobs	Shannon指数	Simpson指数	ACE	Chao	Coverage
Con	5 d	322	3.36	0.16	340.88	337.58	1
	20 d	315	2.54	0.26	356.76	357.09	1
	40 d	307	3.63	0.06	325.73	328.58	1
	平均值	314.67^a	3.18^a	0.16^a	341.12^a	341.08^a	1
Cap	5 d	259	3.4	0.06	290.74	284.08	1
	20 d	231	2.42	0.22	271.95	305.25	1
	40 d	221	1.98	0.32	282.86	280.18	1
	平均值	237^b	2.6^a	0.2^a	281.85^b	289.84^b	1
FJ	5 d	268	2.95	0.13	333.01	329.09	1
	20 d	341	3.45	0.14	351.15	352.04	1
	40 d	336	4.06	0.06	339.69	339.14	1
	平均值	315^a	3.49^a	0.11^a	341.28^a	340.09^a	1

样品		Sobs	Shannon指数	Simpson指数	ACE	Chao	Coverage
Con	5 d	322	3.36	0.16	340.88	337.58	1
	20 d	315	2.54	0.26	356.76	357.09	1
	40 d	307	3.63	0.06	325.73	328.58	1
	平均值	314.67^a	3.18^a	0.16^a	341.12^a	341.08^a	1
Cap	5 d	259	3.4	0.06	290.74	284.08	1
	20 d	231	2.42	0.22	271.95	305.25	1
	40 d	221	1.98	0.32	282.86	280.18	1
	平均值	237^b	2.6^a	0.2^a	281.85^b	289.84^b	1
FJ	5 d	268	2.95	0.13	333.01	329.09	1
	20 d	341	3.45	0.14	351.15	352.04	1
	40 d	336	4.06	0.06	339.69	339.14	1
	平均值	315^a	3.49^a	0.11^a	341.28^a	340.09^a	1

变量	参数	Sobs	ACE	Chao
COD去除率	Pearson相关系数	0.837^**	0.854^**	0.868^**
	显著性	0.005	0.003	0.002
	Spearman相关系数	0.850^**	0.717^*	0.800^**
	显著性	0.004	0.030	0.010
NH₄⁺去除率	Pearson相关系数	0.755^*	0.784^*	0.829^**
	显著性	0.019	0.012	0.006
	Spearman相关系数		0.667^*
	显著性		0.050
TN去除率	Pearson相关系数	0.892^**	0.889^**	0.938^**
	显著性	0.001	0.001	0.000
	Spearman相关系数	0.933^**	0.717^*	0.783^*
	显著性	0.000	0.030	0.013

变量	参数	Sobs	ACE	Chao
COD去除率	Pearson相关系数	0.837^**	0.854^**	0.868^**
	显著性	0.005	0.003	0.002
	Spearman相关系数	0.850^**	0.717^*	0.800^**
	显著性	0.004	0.030	0.010
NH₄⁺去除率	Pearson相关系数	0.755^*	0.784^*	0.829^**
	显著性	0.019	0.012	0.006
	Spearman相关系数		0.667^*
	显著性		0.050
TN去除率	Pearson相关系数	0.892^**	0.889^**	0.938^**
	显著性	0.001	0.001	0.000
	Spearman相关系数	0.933^**	0.717^*	0.783^*
	显著性	0.000	0.030	0.013

类型	属	Con			Cap			FJ
类型	属	5 d	20 d	40 d	5 d	20 d	40 d	5 d	20 d	40 d
PAO	Tetrasphaera	0.04	0.05	0.03	0.03	0.01	0.01	0	0.18	0.04
	Dechloromonas	0.05	0.07	0.05	0.05	0.05	0.03	0.02	0.34	0.16
	总计	0.10±0.02^a			0.06±0.02^a			0.25±0.2^a
GAO	Defluviicoccus	2.66	1.10	5.82	2.03	2.24	2.19	1.13	14.90	6.38
	Micropruina	2.18	1.33	1.32	0.38	0.64	1.12	2.10	3.46	0.39
	总计	4.80±1.92^a			2.87±0.37^a			9.45±6.46^a
PAO/GAO	总计	0.61±0.15^a			0.76±0.08^a			0.70±0.25
AOB & NOB	Nitrospira	0.03	0.09	0	0	0	0	0	0.14	0.10
AOB & NOB	总计	0.04±0.04^a			0^a			0.08±0.06^a
NIR	Acidobacterium	0.01	0.02	0.01	0	0	0	0	0	0.05
	Acidovorax	0.01	0	0.02	0.02	0	0.01	0	0	0
	Anaerolinea	0	0	0.01	0	0	0	0	0.01	0.04
	Bradyrhizobium	0.18	0.37	0.16	0.09	0.03	0.02	0.03	0.35	0.31
	Dechloromonas	0.05	0.07	0.05	0.05	0.05	0.03	0.02	0.34	0.16
	Defluviicoccus	2.66	1.10	5.82	2.03	2.24	2.19	1.13	14.90	6.38
	Haliangium	0.02	0.06	0.05	0.01	0	0	0	0.43	0.89
	Iamia	0.11	0.09	0.06	0.01	0.03	0.01	0.03	0.09	0.07
	Nitrospira	0.03	0.09	0	0	0	0	0	0.14	0.10
	Gordonia	0.13	0.13	0.15	0.20	0.03	0.03	0.09	0.19	0.18
	Hyphomicrobium	0.06	0.01	0.09	0.11	0.33	0.40	0.15	0.01	0
	Leucobacter	0.20	0.01	0.01	0.16	0	0	0.01	0.03	0.04
	Micropruina	2.18	1.33	1.32	0.38	0.64	1.12	2.10	3.46	0.39
	Thermomonas	0.74	0.05	3.45	2.28	0	0.02	0.14	0.12	0.09
	总计	6.99±3.24^a			4.18±0.84^a			10.83±6.85^a
HET	Amaricoccus	0.05	0	0.19	0.06	0.03	0	0.02	0.04	0.02
	Dokdonella	0.01	0.01	0.03	0.01	0	0.01	0.01	0.02	0.03
	E1erococcus	0	0	0.02	0	0.02	0	0	0.03	0.06
	Flavobacterium	0.01	0	0.08	0.03	0	0	0	0.02	0.03
	Mycobacterium	0.19	0.06	0.12	0.10	0.32	0.14	0.19	0.12	0.24
	Nocardioides	0.08	0.18	0.09	0.05	0.09	0.09	0.05	0.40	0.28
	Propioniciclava	0.04	0	0.02	0	0.01	0	0	0.01	0.04
	Simplicispira	0.01	0	0.10	0.03	0	0	0	0.04	0.16
	Sorangium	0	0	0	0	0	0	0	0.02	0.09
	Sphaerotilus	0.02	0	0	0.01	0.01	0	0	0.02	0.03
	Terrimonas	0.21	0.05	0	0	0	0	0	0.02	0.03
	Uliginosibacterium	1.21	0.39	0	0	0	0	0.01	0	0
	Acidobacterium	0.01	0.02	0.01	0	0	0	0	0	0.05
	Acidovorax	0.01	0	0.02	0.02	0	0.01	0	0	0
	Bradyrhizobium	0.18	0.37	0.16	0.09	0.03	0.02	0.03	0.35	0.31
	Dechloromonas	0.05	0.07	0.05	0.05	0.05	0.03	0.02	0.34	0.16
	Defluviicoccus	2.66	1.10	5.82	2.03	2.24	2.19	1.13	14.90	6.38
	Haliangium	0.02	0.06	0.05	0.01	0	0	0	0.43	0.89
	Iamia	0.11	0.09	0.06	0.01	0.03	0.01	0.03	0.09	0.07
	Nitrospira	0.03	0.09	0	0	0	0	0	0.14	0.10
	Ferruginibacter	0.15	0.13	0.16	0.13	0	0	0	0.51	0.60
	Novosphingobium	3.85	3.08	2.57	1.91	0.68	1.45	8.64	1.25	1.76
	Sphingopyxis	0.73	0.18	0	0.06	0	0	0.01	0.03	0
	Tetrasphaera	0.04	0.05	0.03	0.03	0.01	0.01	0	0.18	0.04
	Gordonia	0.13	0.13	0.15	0.20	0.03	0.03	0.09	0.19	0.18
	Hyphomicrobium	0.06	0.01	0.09	0.11	0.33	0.40	0.15	0.01	0
	Leucobacter	0.20	0.01	0.01	0.16	0	0	0.01	0.03	0.04
	Micropruina	2.18	1.33	1.32	0.38	0.64	1.12	2.10	3.46	0.39
	Thermomonas	0.74	0.05	3.45	2.28	0	0.02	0.14	0.12	0.09
	总计	11.71±3.06^a			5.95±1.34b			15.84±4.92^a
总计		13.63±3.49^a			7.84±3.54b			16.06±4.87^a

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